Ring-contracted morphinans and the use thereof

ABSTRACT

The application is directed to compounds of Formula (I) or (IA) and pharmaceutically acceptable salts and solvates thereof, wherein R 1  R 2 , R 3  and G are defined as set forth in the specification. The invention is also directed to use of compounds of Formula (I) or (IA), and the pharmaceutically acceptable salts and solvates thereof, to treat disorders responsive to the modulation of one or more opioid receptors, or as synthetic intermediates. Certain compounds of the present invention are especially useful for treating pain.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is the U.S. national phase, pursuant to 35 U.S.C. §371, of PCT International Application Ser. No. PCT/US2014/071605, filed on Dec. 19, 2014, designating the United States and published in English on Jul. 2, 2015 as publication WO 2015/100174 A1, which claims priority to U.S. Provisional Application Ser. No. 61/920,933, filed on Dec. 26, 2013. The contents of the afore-mentioned patent applications are incorporated herein by their entirety.

BACKGROUND OF THE INVENTION

Field of the Invention

This application is in the field of medicinal chemistry. The application relates to novel ring-contracted morphinans and pharmaceutical compositions comprising one or more of these compounds. The application also relates to methods of using ring-contracted morphinans.

Description of the Related Art

Pain is the most common symptom for which patients seek medical advice and treatment. While acute pain is usually self-limited, chronic pain can persist for 3 months or longer and lead to significant changes in a patient's personality, lifestyle, functional ability and overall quality of life (K. M. Foley, Pain, in Cecil Textbook of Medicine 100-107, J. C. Bennett and F. Plum eds., 20th ed. 1996).

Pain has traditionally been managed by administering either a non-opioid analgesic (such as acetylsalicylic acid, choline magnesium trisalicylate, acetaminophen, ibuprofen, fenoprofen, diflunisal or naproxen), or an opioid analgesic (such as morphine, hydromorphone, methadone, levorphanol, fentanyl, oxycodone, oxymorphone, or buprenorphine).

Until recently, there was evidence of three major classes of opioid receptors in the central nervous system (CNS), with each class having subtype receptors. These receptor classes are known as μ, δ and κ. As opiates have a high affinity to these receptors while not being endogenous to the body, research followed in order to identify and isolate the endogenous ligands to these receptors. These ligands were identified as endorphins, enkephalins, and dynorphins, respectively. Additional experimentation has led to the identification of the opioid receptor-like (ORL-1) receptor, which has a high degree of homology to the known opioid receptor classes. This more recently discovered receptor was classified as an opioid receptor based only on structural grounds, as the receptor did not exhibit pharmacological homology. It was initially demonstrated that non-selective ligands having a high affinity for μ, δ and κ receptors had low affinity for the ORL-1 receptor. This characteristic, along with the fact that an endogenous ligand had not yet been discovered, led to the ORL-1 receptor being designated as an “orphan receptor”.

Kappa (κ) opioid receptor agonists have been evaluated as alternatives to existing analgesics for the treatment of pain. Centrally penetrating κ agonists produce antinociceptive effects in conventional preclinical assays of basal, inflammatory and neuropathic pain (Vanderah et al., J. Pharmacol. Exp. Ther. 310:326-333 (2004); Negus et al., Psychopharmacology (Berl) 210:149-159 (2010)). However, centrally penetrating κ agonists can also produce undesirable side-effects, such as sedative and psychotomimetic effects (Pande et al., Clin. Neuropharmacol. 19:92-97 (1996); Pande et al., Clin. Neuropharmacol. 19:451-456 (1996); and Wadenberg, CNS Drug Rev. 9:187-198 (2003)).

Opioid receptor agonists that do not readily cross the blood-brain barrier are peripherically restricted and distribute poorly to the central nervous system after systemic administration. Such compounds would retain an ability to produce analgesia by acting on peripheral opioid receptors, such as peripheral κ-opioid receptors, but their potency to produce centrally mediated side-effects would be reduced.

There is a need for effective analgesics that work by acting on opioid receptors. There is also a need for analgesics that work by acting on peripheral opioid receptors. There is also a need for analgesics that work by acting on central opioid receptors. There is also a need for analgesics that work by acting on κ-opioid receptors. There is also a need for analgesics that work by acting-on peripheral κ-opioid receptors.

BRIEF SUMMARY OF THE INVENTION

In one aspect, the present disclosure provides compounds represented by Formulae I, IA, and II-XVI, below, and the pharmaceutically acceptable salts and solvates thereof, collectively referred to herein as “Compounds of the Invention” (each is individually referred to hereinafter as a “Compound of the Invention”).

In another aspect, the present disclosure provides the use of Compounds of the Invention as synthesis intermediates.

In another aspect, the present disclosure provides the use of Compounds of the Invention as modulators of one or more opioid receptors. Specifically, the present disclosure provides the use of Compounds of the Invention as modulators of μ, δ, κ, and/or ORL-1 opioid receptors, and especially modulators of μ and/or κ opioid receptors.

In another aspect, the present disclosure provides a method of treating or preventing a disorder responsive to the modulation of one or more opioid receptors in a patient, comprising administering to the patient an effective amount of a Compound of the Invention.

In another aspect, the present disclosure provides a use of a Compound of the Invention as an analgesic to treat or prevent pain; or as an agent to treat or prevent withdrawal from alcohol or drug addiction; or as an agent to treat or prevent addictive disorders; or as an agent to treat a pruritic condition; or as an agent to treat or prevent constipation; or as an agent to treat or prevent diarrhea (each of pain, alcohol withdrawal, drug withdrawal, addictive disorders, pruritis, constipation, and diarrhea being a “Condition”).

The present invention further provides methods of treating or preventing a Condition, comprising administering to a patient in need thereof a therapeutically effective amount of a Compound of the Invention. In certain embodiments, the Condition is pain (including acute pain, chronic pain (which includes but is not limited to, neuropathic pain, postoperative pain, and inflammatory pain), and surgical pain). The Compounds of the Invention are particularly useful for treating or preventing chronic pain.

In another aspect, the present disclosure provides a pharmaceutical composition comprising a therapeutically effective amount of a Compound of the Invention and one or more pharmaceutically acceptable carriers. Such compositions are useful for treating or preventing a Condition in a patient.

In another aspect, the present disclosure provides Compounds of the Invention for use in treatment or prevention of a disorder responsive to the modulation of one or more opioid receptors. Preferably, the disorder is responsive to modulation of the μ-opioid receptor or the κ-opioid receptor, or to modulation of both the μ-opioid receptor and the κ-opioid receptor.

In another aspect, the present disclosure provides a method of modulating one or more opioid receptors in a patient in need of said modulation, comprising administering to the patient an opioid receptor modulating amount of a Compound of the Invention.

In another aspect, the present disclosure provides Compounds of the Invention for use in treatment or prevention of one or more Conditions in a patient in need of said treatment or prevention.

In another aspect, the present disclosure provides Compounds of the Invention for use in treatment or prevention of pain in a patient, such as acute pain, chronic pain (which includes but is not limited to, neuropathic pain, postoperative pain, and inflammatory pain), or surgical pain.

In another aspect, the present disclosure provides Compounds of the Invention for use in modulation of one or more opioid receptors in a patient.

In another aspect, the present disclosure provides use of Compounds of the Invention in the manufacture of a medicament for treating or preventing a disorder responsive to the modulation of one or more opioid receptors.

In another aspect, the present disclosure provides use of Compounds of the Invention in the manufacture of a medicament for modulating of one or more opioid receptors in a patient. Preferably, the μ- or κ-opioid receptor is modulated, or both the μ- and κ-opioid receptors are modulated.

In another aspect, the present disclosure provides Compounds of the Invention for use as a medicament.

In another aspect, the present disclosure provides use of a Compound of the Invention in the manufacture of a medicament for treating or preventing a Condition in a patient.

In another aspect, the present disclosure provides use of a Compound of the Invention in the manufacture of a medicament for treating or preventing pain in a patient, such as acute pain, chronic pain, or surgical pain.

In another aspect, the present disclosure provides a pharmaceutical composition, comprising a Compound of the Invention for treating or preventing a disorder responsive to the modulation of one or more opioid receptors.

The present invention further provides methods for preparing a pharmaceutical composition, comprising admixing a Compound of the Invention and a pharmaceutically acceptable carrier to form the pharmaceutical composition.

In another aspect, the present invention provides radiolabeled Compounds of the Invention, especially ¹H, ¹¹C and ¹⁴C radiolabeled Compounds of the Invention, and the use of such compounds as radioligands to detect binding to an opioid receptor in screening assays.

In another aspect, the present invention provides a method for screening a candidate compound for the ability to bind to an opioid receptor, comprising a) introducing a fixed concentration of a radiolabeled Compound of the Invention to the receptor under conditions that permit binding of the radiolabeled compound to the receptor to form a complex; b) titrating the complex with a candidate compound; and c) determining the binding of the candidate compound to said receptor.

In a further aspect, the invention relates to a kit, comprising a sterile container containing an effective amount of a Compound of the Invention and instructions for therapeutic use.

Additional embodiments and advantages of the disclosure will be set forth, in part, in the description that follows, and will flow from the description, or can be learned by practice of the disclosure. The embodiments and advantages of the disclosure will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims.

It is to be understood that both the foregoing summary and the following detailed description are exemplary and explanatory only, and are not restrictive of the invention as claimed.

DETAILED DESCRIPTION OF THE INVENTION

Certain Compounds of the Invention are useful for modulating a pharmacodynamic response from one or more opioid receptors (μ, δ, κ, ORL-1) either centrally or peripherally, or both. The pharmacodynamic response may be attributed to the compound either stimulating (agonizing) or inhibiting (antagonizing) the one or more receptors. Certain Compounds of the Invention may antagonize one or more opioid receptors, while also agonizing one or more other receptors. Compounds of the Invention having agonist activity may be either full or partial agonists.

One aspect of the invention is based on the use of certain Compounds of the Invention as synthesis intermediates.

In one embodiment, Compounds of the Invention are compounds represented by Formula I:

and the pharmaceutically acceptable salts and solvates thereof, wherein:

R¹ is hydrogen, hydroxy, halo, cyano, carboxy, or aminocarbonyl; or alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; or —O-PG, wherein PG is a hydroxyl protecting group;

R² is

(a) hydrogen or carboxamido; or

(b) alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, alkylcarbonyl, alkoxycarbonyl, (arylalkoxy)carbonyl, or (heteroarylalkoxy)carbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups;

R³ is hydrogen, hydroxy, or halo; or alkoxy, alkylamino, or dialkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups;

each R⁴ is independently selected from the group consisting of hydroxy, halo, alkyl, haloalkyl, cyano, nitro, amino, alkylamino, dialkylamino, carboxy, alkoxy, and alkoxycarbonyl;

G is selected from the group consisting of G¹, G², G³, and G⁴, wherein

G¹ is —C(═O)OR⁵,

G² is —C(═O)NR⁶R⁷,

G³ is —NR⁸R⁹, and

G⁴ is —CN, wherein

R⁵ is hydrogen or alkyl;

R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylkyl, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, and guanidinoalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; or

R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and

R⁸ and R⁹ are each independently selected form the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, (cycloalkyl)-C(═O)—, (cycloalkenyl)-C(═O)—, heterocyclo-C(═O)—, aryl-C(═O)—, heteroaryl-C(═O)—, (cycloalkyl)alkyl-C(═O)—, (cycloalkenyl)alkyl-C(═O)—, (heterocyclo)alkyl-C(═O)—, arylalkyl-C(═O)—, (heteroaryl)alkyl-C(═O)—, (cycloalkyl)-NR¹⁰—C(═O)—, (cycloalkenyl)-NR¹⁰—C(═O)—, heterocyclo-NR¹⁰—C(═O)—, aryl-NR¹⁰—C(═O)—, heteroaryl-NR¹⁰—C(═O)—, (cycloalkyl)alkyl-NR¹⁰—C(═O)—, (cycloalkenyl)alkyl-NR¹⁰—C(═O)—, (heterocyclo)alkyl-NR¹⁰—C(═O)—, arylalkyl-NR¹⁰—C(═O)—, (heteroaryl)alkyl-NR¹⁰—C(═O)—, (cycloalkyl)-SO₂—, (cycloalkenyl)-SO₂—, heterocyclo-SO₂—, aryl-SO₂—, heteroaryl-SO₂—, (cycloalkyl)alkyl-SO₂—, (cycloalkenyl)alkyl-SO₂—, (heterocyclo)alkyl-SO₂—, arylalkyl-SO₂—, (heteroaryl)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, and guanidinoalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups;

R¹⁰ is hydrogen or alkyl;

R^(10a) is alkyl, alkenyl, or alkenyl; or

R¹⁰ and R^(10a) together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and

R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring.

In another embodiment, the invention provides compounds represented by Formula IA:

and the pharmaceutically acceptable salts and solvates thereof, wherein:

R¹ is hydrogen, hydroxy, halo, cyano, carboxy, or aminocarbonyl; or alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; or —O-PG, wherein PG is a hydroxyl protecting group;

R² is

(a) hydrogen or carboxamido; or

(b) alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, alkylcarbonyl, alkoxycarbonyl, (arylalkoxy)carbonyl, or (heteroarylalkoxy)carbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups;

R³ is hydrogen, hydroxy, or halo; or alkoxy, alkylamino, or dialkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups;

each R⁴ is independently selected from the group consisting of hydroxy, halo, alkyl, haloalkyl, cyano, nitro, amino, alkylamino, dialkylamino, carboxy, alkoxy, and alkoxycarbonyl;

G is selected from the group consisting of G¹, G², G³, and G⁴, wherein

G¹ is —C(═O)OR⁵,

G² is —C(═O)NR⁶R⁷,

G³ is —NR⁸R⁹, and

G⁴ is —CN, wherein

R⁵ is hydrogen or alkyl;

R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, guanidinoalkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; or

R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and

R⁸ and R⁹ are each independently selected form the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, (cycloalkyl)-C(═O)—, (cycloalkenyl)-C(═O)—, heterocyclo-C(═O)—, aryl-C(═O)—, heteroaryl-C(═O)—, (cycloalkyl)alkyl-C(═O)—, (cycloalkenyl)alkyl-C(═O)—, (heterocyclo)alkyl-C(═O)—, arylalkyl-C(═O)—, (heteroaryl)alkyl-C(═O)—, (cycloalkyl)-NR¹⁰—C(═O)—, (cycloalkenyl)-NR¹⁰—C(═O)—, heterocyclo-NR¹⁰—C(═O)—, aryl-NR¹⁰—C(═O)—, heteroaryl-NR¹⁰—C(═O)—, (cycloalkyl)alkyl-NR¹⁰—C(═O)—, (cycloalkenyl)alkyl-NR¹⁰—C(═O)—, (heterocyclo)alkyl-NR¹⁰—C(═O)—, arylalkyl-NR¹⁰—C(═O)—, (heteroaryl)alkyl-NR¹⁰—C(═O)—, (cycloalkyl)-SO₂—, (cycloalkenyl)-SO₂—, heterocyclo-SO₂—, aryl-SO₂—, heteroaryl-SO₂—, (cycloalkyl)alkyl-SO₂—, (cycloalkenyl)alkyl-SO₂—, (heterocyclo)alkyl-SO₂—, arylalkyl-SO₂—, (heteroaryl)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, guanidinoalkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups;

R¹⁰ is hydrogen or alkyl;

R^(10a) is alkyl, alkenyl, or alkenyl; or

R¹⁰ and R^(10a) together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and

R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring.

In another embodiment, Compounds of the Invention are compounds represented by Formula II:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula III:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula IV:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula V:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula VI:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula VII:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula VIII:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by Formula IX:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds represented by any one of Formulae X to XV:

and the pharmaceutically acceptable salts and solvates thereof, wherein R¹, R², R³, and G are as defined for Formula I or IA.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is H, hydroxy, halo, cyano, carboxy, or aminocarbonyl (i.e., —C(═O)NH₂). In another embodiment, R¹ is hydroxy, cyano, or aminocarbonyl. In another embodiment, R¹ is hydroxy. In another embodiment, R¹ is cyano. In another embodiment, R¹ is aminocarbonyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy, or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. In another embodiment, R¹ is C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₁₋₆ alkoxy, C₂₋₆ alkenyloxy, or C₂₋₆ alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, halo(C₁₋₆)alkyl, amino, C₁₋₆ alkylamino, di(C₁₋₆)alkylamino, carboxy, C₁₋₆ alkoxy, C₁₋₆ alkoxycarbonyl, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, 5- or 6-membered heterocyclo, C₃₋₇ cycloalkyl, and C₃₋₇ cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. Useful R⁴ groups include hydroxy, halo, C₁₋₆ alkyl, halo(C₁₋₆)alkyl, cyano, nitro, amino, C₁₋₆ alkylamino, di(C₁₋₆)alkylamino, carboxy, C₁₋₆ alkoxy, and C₁₋₆ alkoxycarbonyl, and preferably hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, C₁₋₄ alkylamino, di(C₁₋₄ alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl, such as hydroxy, methyl, ethyl, halo, trifluoromethyl, cyano, nitro, amino, methylamino, ethylamino, dimethylamino, diethylamino, carboxy, methoxy, ethoxy, methoxycarbonyl, and ethoxycarbonyl. In another embodiment, R¹ is C₁₋₆ alkoxy, C₂₋₆ alkenyloxy, or C₂₋₆ alkynyloxy, any of which are optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl. In another embodiment, R¹ is unsubstituted C₁₋₆ alkoxy, unsubstituted C₂₋₆ alkenyloxy, or unsubstituted C₂₋₆ alkynyloxy. In another embodiment, R¹ is unsubstituted methoxy, ethoxy, n-propoxy, iso-propoxy, n-butoxy, tert-butoxy, iso-butoxy, or sec-butoxy, and advantageously R¹ is unsubstituted methoxy. In another embodiment, R¹ is unsubstituted ethenoxy, propenoxy, isopropenoxy, butenoxy, or sec-butenoxy. In another embodiment, R¹ is unsubstituted ethynoxy, propynoxy, butynoxy, or 2-butynoxy.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is unsubstituted C₁₋₆ alkoxy, hydroxy, cyano, or aminocarbonyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is unsubstituted C₁₋₆ alkoxy or hydroxy, and preferably unsubstituted C₁₋₄ alkoxy or hydroxy.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is hydrogen or carboxamido. In this aspect of the invention, preferably R² is hydrogen, —CONH₂, —CON(H)C₁₋₄ alkyl, —CON(C₁₋₄ alkyl)₂, or —CON(H)Ph, and more preferably R² is hydrogen.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, alkylcarbonyl, alkoxycarbonyl, (arylalkoxy)carbonyl, or (heteroarylalkoxy)carbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. In one embodiment, R² is C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, C₃₋₇ cycloalkyl(C₁₋₄)alkyl, C₃₋₇ cycloalkenyl(C₁₋₄)alkyl, 5- or 6-membered heterocyclo(C₁₋₄)alkyl, C₆₋₁₀ aryl(C₁₋₄)alkyl, 5- or 6-membered heteroaryl(C₁₋₄)alkyl, C₁₋₆ alkylcarbonyl, C₁₋₆ alkoxycarbonyl, C₆₋₁₀ aryl(C₁₋₄)alkoxycarbonyl, or 5- or 6-membered heteroaryl(C₁₋₄)alkoxycarbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₆ alkyl, halo, halo(C₁₋₆)alkyl, amino, C₁₋₆ alkylamino, di(C₁₋₆)alkylamino, carboxy, C₁₋₆ alkoxy, C₁₋₆ alkoxycarbonyl, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, 5- or 6-membered heterocyclo, C₃₋₇ cycloalkyl, and C₃₋₇ cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. Useful R⁴ groups are those described above in connection with R¹.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, C₃₋₇ (cycloalkyl)(C₁₋₄ alkyl, C₃₋₇ (cycloalkenyl)(C₁₋₄ alkyl, 5- or 6-membered heterocyclo(C₁₋₄)alkyl, C₆₋₁₀ aryl(C₁₋₄)alkyl, 5- or 6-membered heteroaryl(C₁₋₄ alkyl, C₁₋₄ alkylcarbonyl, C₁₋₄ alkoxycarbonyl, C₆₋₁₀ aryl(C₁₋₄)alkoxycarbonyl, or 5- or 6-membered heteroaryl(C₁₋₄ alkoxycarbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl, and preferably optionally substituted with 1 or 2 substituents, each independently selected from the group consisting of hydroxy, methyl, ethyl, halo, trifluoromethyl, amino, methylamino, ethylamino, dimethylamino, diethylamino, carboxy, methoxy, ethoxy, methoxycarbonyl, and ethoxycarbonyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is C₁₋₆ alkyl, and preferably C₁₋₄ alkyl, which is unsubstituted or substituted with 1, 2, or 3 substituents each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl. In another embodiment, R² is methyl, ethyl, trifluoromethyl, or 2,2,2-trifluoroethyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is unsubstituted C₁₋₆ alkyl, and preferably unsubstituted C₁₋₄ alkyl, such as methyl, ethyl, n-propyl, iso-propyl, n-butyl, or tert-butyl, and more preferably methyl or ethyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is C₃₋₇ (cycloalkyl)(C₁₋₄)alkyl or C₃₋₇ (cycloalkenyl)(C₁₋₄)alkyl, and especially C₃₋₇ (cycloalkyl)(C₁₋₄)alkyl, such as cyclopropyl(C₁₋₄)alkyl, cyclobutyl(C₁₋₄)alkyl, cyclopentyl(C₁₋₄)alkyl, or cyclohexyl(C₁₋₄)alkyl, optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl, and preferably optionally substituted with 1 or 2 substituents, each independently selected from the group consisting of hydroxy, methyl, ethyl, halo, trifluoromethyl, amino, methylamino, ethylamino, dimethylamino, diethylamino, carboxy, methoxy, ethoxy, methoxycarbonyl, and ethoxycarbonyl. Preferably, R² is unsubstituted cyclopropyl(C₁₋₄)alkyl. In another embodiment, R² is unsubstituted cyclohexyl(C₁₋₄)alkyl, such as cyclohexylmethyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is unsubstituted (cyclopropyl)methyl, 2-(cyclopropyl)ethyl or 3-(cyclopropyl)propyl. In another embodiment, R² is unsubstituted (cyclopropyl)methyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is C₁₋₆ alkyl, and preferably C₁₋₄ alkyl, substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of halo (such as fluoro), halo(C₁₋₄)alkyl (such as, for example, trifluoro(C₁₋₂)alkyl), phenyl, and heterocyclo (such as tetrahydropyranyl). In one embodiment of this aspect of the invention, R² is benzyl, phenethyl, 3-phenylpropyl, tetrahydropyran-4-ylmethyl, 2,2,2-trifluoroethyl, 2-fluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, or 2,2-difluoroethyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R² is C₂₋₆ alkenyl, and preferably C₂₋₄ alkenyl, which is unsubstituted or substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of C₁₋₄ alkyl (such as methyl), halo (such as fluoro), halo(C₁₋₄)alkyl (such as, for example, trifluoro(C₁₋₂)alkyl), phenyl, and heterocyclo (such as tetrahydropyranyl). In one embodiment of this aspect of the invention, R² is 3-methyl-but-2-enyl, 3-methylbut-3-enyl, or 4-phenylbut-2-enyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R³ is hydrogen.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R³ is hydroxy or halo. In another embodiment, R³ is hydroxy.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R³ is alkoxy, alkylamino, or dialkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R³ is C₁₋₆ alkoxy, C₁₋₆ alkylamino, or di(C₁₋₆)alkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, halo(C₁₋₆)alkyl, amino, C₁₋₆ alkylamino, di(C₁₋₆)alkylamino, carboxy, C₁₋₆ alkoxy, C₁₋₆ alkoxycarbonyl, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, 5- or 6-membered heterocyclo, C₃₋₇ cycloalkyl, and C₃₋₇ cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. Useful R⁴ groups are those described above in connection with R¹.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R³ is unsubstituted C₁₋₆ alkoxy or C₁₋₆ alkoxy substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G¹, and is —C(═O)OR⁵, wherein R⁵ is hydrogen or alkyl. In one embodiment, R⁵ is hydrogen. In another embodiment, R⁵ is alkyl, and preferably C₁₋₆ alkyl, more preferably C₁₋₄ alkyl, such as methyl or ethyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G¹, and G¹ is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is —C(═O)NR⁶R⁷, wherein R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, (guanidino)alkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups. In another embodiment, R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, phenyl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)-alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)-alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)-alkyl, phenyl(C₁₋₆)alkyl, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆alkyl)amino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆alkoxy)(C₁₋₆)alkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, phenyl, and heteroaryl portions are optionally substituted with one or more R⁴ groups. In another embodiment, R⁶ is hydrogen or C₁₋₆ alkyl, and R⁷ is selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, phenyl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl, phenyl(C₁₋₆)alkyl, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆ alkyl)amino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆ alkoxy)(C₁₋₆)alkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, phenyl, and heteroaryl portions are optionally substituted with one or more R⁴ groups. Suitable R⁴ groups include those defined in connection with R¹.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

wherein m is independently 0, 1, 2, or 3, and

R¹¹, R¹², and R¹³ are each independently selected from the group consisting of hydrogen, hydroxy, halogen, and C₁₋₄ alkyl. In one embodiment, m is 0. In another embodiment, m is 1. In another embodiment, m is 2. In another embodiment, m is 3. In one embodiment, R¹¹, R¹², and R¹³ each are hydrogen. In another embodiment, R¹¹ is hydrogen and R¹² and R¹³ are each independently selected from the group consisting of hydroxy, halogen (such as fluoro and chloro), and C₁₋₄ alkyl (such as methyl or ethyl). In another embodiment, R¹¹ and R¹² both are hydrogen, and R¹³ is selected from the group consisting of hydroxy, halogen (such as fluoro and chloro), and C₁₋₄ alkyl (such as methyl or ethyl).

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

wherein m is independently 0, 1, 2, or 3, and

R¹¹, R¹², and R¹³ are each independently selected from the group consisting of hydrogen, hydroxy, halogen, and C₁₋₄ alkyl. In one embodiment, m is 0. In another embodiment, m is 1. In another embodiment, m is 2. In another embodiment, m is 3. In one embodiment, R¹¹, R¹², and R¹³ each are hydrogen. In another embodiment, R¹¹ is hydrogen and R¹² and R¹³ are each independently selected from the group consisting of hydroxy, halogen (such as fluoro and chloro), and C₁₋₄ alkyl (such as methyl or ethyl). In another embodiment, R¹¹ and R¹² both are hydrogen, and R¹³ is selected from the group consisting of hydroxy, halogen (such as fluoro and chloro), and C₁₋₄ alkyl (such as methyl or ethyl).

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is

wherein m, R¹¹, R¹², and R¹³ are as defined above.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is

wherein m, R¹¹, R¹², and R¹³ are as defined above.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is

wherein m, R¹¹, R¹², and R¹³ are as defined above.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is

wherein R¹¹, R¹², and R¹³ are as defined above, and m is 1, 2, or 3. In another embodiment, G² is

wherein R¹¹ and R¹² are as defined above, and m is 1, 2, or 3.

In another embodiment, G² is

and m is 1, 2, or 3.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and is —C(═O)NR⁶R⁷, wherein R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring. In one embodiment, the heterocyclic ring is optionally substituted with 1, 2, or 3 independently selected substituents. In one embodiment, the optionally substituted heterocyclic ring is an optionally substituted 3-7 membered heterocyclic ring, and preferably an optionally substituted 5- or 6-membered heterocyclic ring. In this aspect of the invention, useful heterocyclic rings include unsubstituted or substituted 5- or 6-membered heterocyclic rings containing 1 or 2 nitrogen atoms and optionally 1 or 2 heteroatoms selected from the group consisting of O, S, and NR¹⁴, wherein R¹⁴ is selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₆₋₁₀ aryl, and 5- or 6-membered heteroaryl, wherein said aryl and heteroaryl groups are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. In one embodiment, the heterocyclic ring is a substituted or unsubstituted 5-membered heterocyclic ring containing 1 nitrogen atom, such as substituted or unsubstituted pyrrolidin-1-yl. In another embodiment, the heterocyclic ring is a substituted or unsubstituted 6-membered heterocyclic ring containing 1 nitrogen atom and optionally 1 heteroarom selected from the group consisting of O, S, and NR¹⁴, wherein R¹⁴ is as defined above, such as, for example, a substituted or unsubstituted piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, and dioxidothiomorpholinyl. In another embodiment, the optionally substituted heterocyclic ring is an optionally substituted bicyclic ring system. In this aspect of the invention, suitable heterocyclic rings include unsubstituted or substituted 7-10 membered bicyclic ring systems containing 1 or 2 nitrogen atoms and optionally 1 or 2 heteroaroms selected from the group consisting of O, S, or NR¹⁴, wherein R¹⁴ is as defined above, such as, for example, isoindolin-2-yl and azabicyclo[3.2.1]octan-8-yl. Suitable optional substituents include hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, and 5- or 6-membered heteroaryl, wherein said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; and preferably suitable optional substituents include hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₆ cycloalkyl, C₃₋₆ cycloalkenyl, pyrrolidin-1-yl, piperidin-1-yl, and phenyl, wherein said phenyl is optionally substituted with 1, 2, or 3 independently selected R⁴ groups.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², wherein R⁶ and R⁷ together with the nitrogen atom to which they are attached form a pyrrolidinyl, a piperidinyl, a piperazinyl, a morpholinyl, a thiomorpholinyl, or a dioxidothiomorpholinyl ring, which is unsubstituted or substituted with 1, 2, or 3 substituents each independently selected from the group consisting of hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₆ cycloalkyl, C₃₋₆ cycloalkenyl, pyrrolidin-1-yl, piperidin-1-yl, and phenyl, wherein said phenyl is optionally substituted with 1, 2, or 3 independently selected R⁴ groups.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G², and G² is

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, and is —NR⁸R⁹, wherein R⁸ and R⁹ are each independently selected form the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, (cycloalkyl)-C(═O)—, (cycloalkenyl)-C(═O)—, heterocyclo-C(═O)—, aryl-C(═O)—, heteroaryl-C(═O)—, (cycloalkyl)alkyl-C(═O)—, (cycloalkenyl)alkyl-C(═O)—, (heterocyclo)alkyl-C(═O)—, arylalkyl-C(═O)—, heteroarylalkyl-C(═O)—, (cycloalkyl)-NR¹⁰—C(═O)—, (cycloalkenyl)-NR¹⁰—C(═O)—, heterocyclo-NR¹⁰—C(═O)—, aryl-NR¹⁰—C(═O)—, heteroaryl-NR¹⁰—C(═O)—, (cycloalkyl)alkyl-NR¹⁰—C(═O)—, (cycloalkenyl)alkyl-NR¹⁰—C(═O)—, (heterocyclo)alkyl-NR¹⁰—C(═O)—, arylalkyl-NR¹⁰—C(═O)—, (heteroaryl)alkyl-NR¹⁰—C(═O)—, (cycloalkyl)-SO₂—, (cycloalkenyl)-SO₂—, heterocyclo-SO₂—, aryl-SO₂—, heteroaryl-SO₂—, (cycloalkyl)alkyl-SO₂—, (cycloalkenyl)alkyl-SO₂—, (heterocyclo)alkyl-SO₂—, arylalkyl-SO₂—, (heteroaryl)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, guanidinoalkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; and wherein R¹⁰ is hydrogen or alkyl, and R^(10a) is alkyl, alkenyl, or alkynyl; or R¹⁰ and R^(10a) together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, wherein R⁸ and R⁹ are each independently selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl, C₆₋₁₀ aryl(C₁₋₆)alkyl, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkyl)-C(═O)—, (C₃₋₇ cycloalkenyl)-C(═O)—, (5- or 6-membered heterocyclo)-C(═O)—, C₆₋₁₀ aryl-C(═O)—, (5- or 6-membered heteroaryl)-C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkyl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)-NR¹⁰—C(═O)—, C₆₋₁₀ aryl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)-SO₂—, (C₃₋₇ cycloalkenyl)-SO₂—, (5- or 6-membered heterocyclo)-SO₂—, C₆₋₁₀ aryl-SO₂—, (5- or 6-membered heteroaryl)-SO₂—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-SO₂—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-SO₂—, C₆₋₁₀ aryl(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆)alkylamino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆)alkoxy(C₁₋₆)alkyl, wherein R¹⁰ is hydrogen or C₁₋₆ alkyl, and R^(10a) is C₁₋₆ alkyl, C₂₋₆ alkenyl, or C₂₋₆ alkynyl, or R¹⁰ and R^(10a) together form a 5- or 6-membered heterocyclic ring optionally substituted with 1, 2, or 3 independently selected R⁴ groups; and wherein said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions thereof are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. Suitable R⁴ groups are those described in connection with R¹.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, wherein R⁸ and R⁹ are both hydrogen. In this embodiment, G is —NH₂.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, wherein R⁸ is hydrogen and R⁹ is selected from the group consisting of C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl, C₆₋₁₀ aryl(C₁₋₆)alkyl, and (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkyl)-C(═O)—, (C₃₋₇ cycloalkenyl)-C(═O)—, (5- or 6-membered heterocyclo)-C(═O)—, C₆₋₁₀ aryl-C(═O)—, (5- or 6-membered heteroaryl)-C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkyl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)-NR¹⁰—C(═O)—, C₆₋₁₀ aryl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)-SO₂—, (C₃₋₇ cycloalkenyl)-SO₂—, (5- or 6-membered heterocyclo)-SO₂—, C₆₋₁₀ aryl-SO₂—, (5- or 6-membered heteroaryl)-SO₂—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-SO₂—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-SO₂—, C₆₋₁₀ aryl(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆)alkylamino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆)alkoxy(C₁₋₆)alkyl, wherein R¹⁰ is hydrogen or C₁₋₆ alkyl, and R^(10a) is C₁₋₆ alkyl, C₂₋₆ alkenyl, or C₂₋₆ alkynyl, or R¹⁰ and R^(10a) together form a 5- or 6-membered heterocyclic ring optionally substituted with 1, 2, or 3 independently selected R⁴ groups; and wherein said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions thereof are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. Suitable R⁴ groups are those described in connection with R¹. In another embodiment, R⁹ is selected from the group consisting of

wherein R¹⁵ is hydrogen, halogen, or C₁₋₄ alkyl. In one embodiment, R¹⁵ is hydrogen. In another embodiment, R⁹ is

wherein R¹⁵ is hydrogen, halogen, or C₁₋₄ alkyl. In one embodiment, R¹⁵ is hydrogen.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R⁸ and R⁹ are each independently selected from the group consisting of C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl, C₆₋₁₀ aryl(C₁₋₆)alkyl, and (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkyl)-C(═O)—, (C₃₋₇ cycloalkenyl)-C(═O)—, (5- or 6-membered heterocyclo)-C(═O)—, C₆₋₁₀ aryl-C(═O)—, (5- or 6-membered heteroaryl)-C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-C(═O)—, (C₃₋₇ cycloalkyl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)-NR¹⁰—C(═O)—, C₆₋₁₀ aryl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, C₆₋₁₀ aryl(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-NR¹⁰—C(═O)—, (C₃₋₇ cycloalkyl)-SO₂—, (C₃₋₇ cycloalkenyl)-SO₂—, (5- or 6-membered heterocyclo)-SO₂—, C₆₋₁₀ aryl-SO₂—, (5- or 6-membered heteroaryl)-SO₂—, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl-SO₂—, (C₃₋₇ cycloalkenyl)(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heterocyclo)(C₁₋₆)alkyl-SO₂—, C₆₋₁₀ aryl(C₁₋₆)alkyl-SO₂—, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆)alkylamino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆)alkoxy(C₁₋₆)alkyl, wherein R¹⁰ is hydrogen hydrogen or C₁₋₆ alkyl, and R^(10a) is C₁₋₆ alkyl, C₂₋₆ alkenyl, or C₂₋₆ alkynyl, or R¹⁰ and R^(10a) together form a 5- or 6-membered heterocyclic ring optionally substituted with 1, 2, or 3 independently selected R⁴ groups; and wherein said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions thereof are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. In one embodiment, R⁸ and R⁹ are both

wherein each R¹⁵ is independently selected from the group consisting of hydrogen, halogen, and C₁₋₄ alkyl. In one embodiment, R¹⁵ is hydrogen.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, and is —NR⁸R⁹, wherein R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring. In one embodiment, the optionally substituted heterocyclic ring is an optionally substituted 3-7 membered heterocyclic ring, and preferably an optionally substituted 5- or 6-membered heterocyclic ring. In this aspect of the invention, useful heterocyclic rings include unsubstituted or substituted 5- or 6-membered heterocyclic rings containing 1 or 2 nitrogen atoms and optionally 1 or 2 heteroatoms selected from the group consisting of O, S, and NR¹⁶, wherein R¹⁶ is selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₆₋₁₀ aryl, and 5- or 6-membered heteroaryl, wherein said aryl and heteroaryl groups are optionally substituted with 1, 2, or 3 independently selected R⁴ groups. In one embodiment, the heterocyclic ring is a substituted or unsubstituted 5-membered heterocyclic ring containing 1 nitrogen atom, such as substituted or unsubstituted pyrrolidin-1-yl. In another embodiment, the heterocyclic ring is a substituted or unsubstituted 6-membered heterocyclic ring containing 1 nitrogen atom and optionally 1 heteroarom selected from the group consisting of O, S, and NR¹⁶, wherein R¹⁶ is as defined above, such as, for example, a substituted or unsubstituted piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, and dioxidothiomorpholinyl. In another embodiment, the optionally substituted heterocyclic ring is an optionally substituted bicyclic ring system. In this aspect, suitable heterocyclic rings include unsubstituted or substituted 7-10 membered bicyclic ring systems containing 1 or 2 nitrogen atoms and optionally 1 or 2 heteroaroms selected from the group consisting of O, S, or NR¹⁶, wherein R¹⁶ is as defined above, such as, for example, isoindolin-2-yl and azabicyclo[3.2.1]octan-8-yl. Suitable optional substituents include hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, C₆₋₁₀ aryl, and 5- or 6-membered heteroaryl, wherein said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; and preferably suitable optional substituents include hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₆ cycloalkyl, C₃₋₆ cycloalkenyl, pyrrolidin-1-yl, piperidin-1-yl, and phenyl.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, which is selected from the group consisting of

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G³, which is

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein G is G⁴, which is —CN.

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is hydroxy or unsubstituted C₁₋₆ alkoxy; R² is unsubstituted C₁₋₄ alkyl; C₁₋₄ alkyl substituted with halo(C₁₋₄)alkyl; or cyclopropyl(C₁₋₄)alkyl, cyclobutyl(C₁₋₄ alkyl, cyclopentyl(C₁₋₄)alkyl, or cyclohexyl(C₁₋₄ alkyl, optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl; R³ is hydrogen or hydroxy; and G is as defined in connection with any of Formulae I, IA, and II-XV. In one embodiment, R² is unsubstituted cyclopropyl(C₁₋₄)alkyl, and especially cyclopropylmethyl. In another embodiment, R² is 2,2,2-trifluoroethyl. In another embodiment, R³ is hydroxy.

In another embodiment, Compounds of the Invention are compounds of TABLE 1, and the pharmaceutically acceptable salts and solvates thereof.

TABLE 1 Compd No. Structure Chemical name  6

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2-guanidinoethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide  7

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(4-guanidinobutyl)-3a,8-dibydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 11

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 12

(2R,3aS,4R,9bS)-methyl-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxylate 14

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxylic acid 15

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxylic acid 16

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)-cyclopenta- [a]naphthalene-2-carboxylic acid 19

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-yl)(pyrrolidin-1- yl)methanone 20

((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-yl)(pyrrolidin-1- yl)methanone 21

(2R,3aS,4R,9bS)-2-amino-12- (cyclopropylmethyl)-8-methoxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-3a-ol 22

(2R,3aS,4R,9bS)-2-amino-12- (cyclopropylmethyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-3a,8-diol 25

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carbonitrile 26

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-8-methoxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carbonitrile 28

(2S,3aS,4R,9bS)-2-amino-12- (cyclopropylmethyl)-8-methoxy- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 3a-ol 29

(2S,3aS,4R,9bS)-2-amino-12- (cyclopropylmethyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-3a,8-diol 30

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 31

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 33

(2R,3aS,4R,9bS)-N-benzyl-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 34

(2S,3aS,4R,9bS)-N-benzyl-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 35

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 36

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxamide 37

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(4-methylbenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 38

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(4-methylbenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 39

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(4-hydroxybenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 40

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(4-hydroxybenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 41

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 42

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 43

(2R,3aS,4R,9bS)-N-(4-chlorobenzyl)-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 44

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 45

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 46

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 47

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 48

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(4-fluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 49

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,5-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 50

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxamide 51

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 56

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(piperidin-1- yl)methanone 57

((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(piperidin-1- yl)methanone 58

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(morpholino)- methanone 59

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N,N-diethyl-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 62

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N,N-dimethyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 64

(2R,3aS,4R,9bS)-N-(2-amino-2-oxoethyl)- 12-(cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 66

2-((2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-N-methyl- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamido)acetic acid 75

(2R,3aS,4R,9bS)-3a,8-dihydroxy-12- (2,2,2-tritluoroethyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 76

(2S,3aS,4R,9bS)-3a,8-dihydroxy-12-(2,2, 2-trifluoroethyl)-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 77

(2R,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 78

(2S,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 79

N-((2S,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalen-2-yl)benzamide 80

(2R,3aS,4R,9bS)-2-(benzylamino)-12- (cyclopropylmethyl)-2,3,4,5- tetrahydro-4,9b-(epiminoethano)- cyclopenta-[a]naphthalene-3a,8(1H)- diol 81

(2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-2-(dibenzylamino)-2,3,4,5- tetrahydro-4,9b-(epiminoethano)- cyclopenta-[a]naphthalene-3a,8(1H)- diol 82

N-((2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalen-2-yl)benzamide 83

1-((2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalen-2-yl)-3-phenylurea 84

(2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-N-(2- morpholinoethyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 85

(2R,3aS,4R,9bS)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-N-(3- morpholinopropyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 86

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(4-phenylpiperazin-1- yl)methanone 87

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(thiomorpholino)- methanone 88

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(4-(pyrrolidin-1- yl)piperidin-1-yl)methanone 89

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(pyridin-3-ylmethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 90

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(pyridin-4-ylmethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 91

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(3-morpholinopropyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 92

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(pyridin-3-ylmethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 93

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(2-morpholinoethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 94

((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(1,1-dioxido- thiomorpholino)methanone

In another embodiment, Compounds of the Invention are compounds of TABLE 2, and the pharmaceutically acceptable salts and solvates thereof.

TABLE 2 Compound No. Structure Chemical name  95

N-((2R,3aS,4R,9bR)-12-(cyclopropyl- methyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalen-2-yl)benzene- sulfonamide  96

((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(4-phenylpiperazin-1- yl)methanone  97

(2S,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-N-(4-methylbenzyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide  98

(2R,3aS,4R,9bS)-N-(2,4-dihydroxy- benzyl)-3a,8-dihydroxy-12-methyl- 1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide  99

(2R,3aS,4R,9bS)-N-(3,4-dichlorobenzyl)- 3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxamide 100

(2R,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-N-phenethyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 101

(2S,3aS,4R,9bS)-N-(3,4-dichlorobenzyl)- 3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 102

(2S,3aS,4R,9bS)-3a,8-dihydroxy-N- isobutyl-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 103

(2R,3aS,4R,9bS)-N-(2,4-dichlorobenzyl)- 3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 104

(2R,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-N-(4-methylbenzyl)-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 105

(2R,3aS,4R,9bS)-N-benzyl-3a,8- dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 106

(2S,3aS,4R,9bS)-N-benzyl-3a,8- dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 107

(2S,3aS,4R,9bS)-N-(2,4-dichlorobenzyl)- 3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 108

(2S,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-N-phenethyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 109

N-((2S,3aS,4R,9bR)-3a,8-dihydroxy-12- methyl-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalen- 2-yl)benzamide 110

N-((2R,3aS,4R,9bR)-3a,8-dihydroxy-12- methyl-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalen- 2-yl)benzamide 111

((2R,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalen- 2-yl)(morpholino)methanone 112

(2R,3aS,4R,9bS)-3a,8-dihydroxy-12- methyl-N-(2-morpholinoethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 113

(2R,3aS,4R,9bS)-N-(2-(dimethylamino)- ethyl)-3a,8-dihydroxy-N,12-dimethyl- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 114

(2R,3aS,4R,9bS)-3a,8-dihydroxy-N-(2- hydroxyethyl)-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 115

(2S,3aS,4R,9bS)-3a,8-dihydroxy-N-(2- hydroxyethyl)-12-methyl-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 116

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-N²-(4-methylbenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2,8- dicarboxamide 117

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-N²-(4-methylbenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2,8- dicarboxamide 118

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-N²-(2-morpholinoethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2,8- dicarboxamide 119

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a-hydroxy-N²-(2-morpholinoethyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2,8- dicarboxamide 120

(2R,3aS,4R,9bS)-8-cyano-12- (cyclopropylmethyl)-3a-hydroxy-N-(4- methylbenzyl)-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 121

(2S,3aS,4R,9bS)-8-cyano-12- (cyclopropylmethyl)-3a-hydroxy-N-(4- methylbenzyl)-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 122

(2R,3aS,4R,9bS)-8-cyano-12- (cyclopropylmethyl)-3a-hydroxy-N-(2- morpholinoethyl)-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 123

(2S,3aS,4R,9bS)-8-cyano-12- (cyclopropylmethyl)-3a-hydroxy-N-(2- morpholinoethyl)-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 124

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,3-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 125

(2R,3aS,4R,9bS)-N-(3-chloro-2- methylbenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 126

(2R,3aS,4R,9bS)-N-(3-chloro-4- methylbenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 127

(2R,3aS,4R,9bS)-N-(2-chloro-4- hydroxybenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 128

(2R,3aS,4R,9bS)-N-(3-chloro-4- hydroxybenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 129

(2R,3aS,4R,9bS)-N-(4-chlorophenethyl)- 12-(cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 130

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-dichlorophenethyl)-3a,8- dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 131

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-dichlorophenethyl)-3a,8- dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 132

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalen-2-yl)(4-isopropylpiperazin- 1-yl)methanone 133

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(2-(piperidin-1- yl)ethyl)-1,2,3,3a,4,5-hexahydro-4,9b- (epiminoethano)cyclopenta[a]naphthalene- 2-carboxamide 134

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2-((2S,6R)-2,6-dimethylmorpholino)- ethyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)- cyclopenta[a]naphthalene-2-carboxamide 135

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2-((2S,6R)-2,6-dimethylmorpholino)- ethyl)-3a,8-dihydroxy-1,2,3,3a,4,5- hexahydro-4,9b-(epiminoethano)cyclo- penta[a]naphthalene-2-carboxamide 136

(2R,3aS,4R,9bS)-N-(4-chloro-2- fluorobenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 137

(2S,3aS,4R,9bS)-N-(3-chloro-4- methylbenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 138

(2S,3aS,4R,9bS)-N-(3-chloro-2- methylbenzyl)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro- 4,9b-(epiminoethano)cyclopenta- [a]naphthalene-2-carboxamide 139

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,5-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 140

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 141

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,3-dihydroxybenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexabydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 142

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(3-hydroxybenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 143

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,5-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 144

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 145

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,4-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 146

(2S,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 147

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,4-difluorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 148

(2R,3aS,4R,9bS)-N-(2-chlorobenzyl)-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 149

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- 3a,8-dihydroxy-N-(2-hydroxybenzyl)- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 150

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(3,5-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 151

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)- N-(2,5-dichlorobenzyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide 152

(2R,3aS,4R,9bS)-N-(3-chlorobenzyl)-12- (cyclopropylmethyl)-3a,8-dihydroxy- 1,2,3,3a,4,5-hexahydro-4,9b-(epimino- ethano)cyclopenta[a]naphthalene-2- carboxamide

In another embodiment, Compounds of the Invention are compounds of any one of Formulae I, IA, and II-XV, wherein R¹ is —O-PG, wherein PG is a hydroxyl protecting group.

In another embodiment, Compounds of the Invention are compounds of Formula I or IA, represented by Formula XVI:

wherein R², R³ and G are as defined for Formula I. Suitable and preferable definitions for R², R³ and G are those described above for any of Formulae I, IA, and II-XV.

Suitable hydroxyl protecting groups for PG are well known and include, for example, any suitable hydroxyl protecting group disclosed in Wuts, P. G. M. & Greene, T. W., Greene's Protective Groups in Organic Synthesis, 4rd Ed., pp. 16-430 (J. Wiley & Sons, 2007), herein incorporated by reference in its entirety. The term “hydroxyl protecting group” as used herein refers to a group that blocks (i.e., protects) the hydroxy functionality while reactions are carried out on other functional groups or parts of the molecule. Those skilled in the art will be familiar with the selection, attachment, and cleavage of protecting groups and will appreciate that many different protective groups are known in the art, the suitability of one protective group or another being dependent on the particular synthetic scheme planned. Suitable hydroxy protecting groups are generally able to be selectively introduced and removed using mild reaction conditions that do not interfere with other portions of the subject compounds. These protecting groups can be introduced or removed at a convenient stage using methods known in the art. The chemical properties of such groups, methods for their introduction and removal are known in the art and can be found, for example, in Greene, T. W. and Wuts, P. G. M., above. Additional hydroxyl protecting groups can be found, for example, in U.S. Pat. No. 5,952,495, U.S. Patent Appl. Pub. No. 2008/0312411, WO 2006/035195, and WO 98/02033, which are herein incorporated by reference in their entireties. Suitable hydroxyl protecting groups include the methoxymethyl, tetrahydropyranyl, tert-butyl, allyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, acetyl, pivaloyl, benzoyl, benzyl (Bn), and p-methoxybenzyl group.

It will be apparent to a person of ordinary skill in the art in view of this disclosure that certain groups included in the definitions of —O-PG overlap with the other definitions for R¹, such as methoxy, tert-butoxy, etc., and, thus, certain Compounds of the Invention having R¹ groups that include groups acting as hydroxyl protecting groups can be pharmaceutically active as described herein.

In one embodiment, the hydroxyl protecting group PG is selected from the group consisting of alkyl, arylalkyl, heterocyclo, (heterocyclo)alkyl, acyl, silyl, and carbonate, any of which are optionally substituted.

In another embodiment, the hydroxyl protecting group PG is an alkyl group, typically an optionally substituted C₁₋₆ alkyl group, and suitably unsubstituted methyl or tert-butyl.

In another embodiment, the hydroxyl protecting group PG is an arylalkyl group. Suitable arylalkyl groups include, for example, an unsubstituted benzyl group, substituted benzyl groups, such as p-methoxybenzyl, and naphthylmethyl.

In another embodiment, the hydroxyl protecting group PG is a heterocyclo group, such as unsubstituted tetrahydropyranyl or optionally substituted tetrahydropyranyl.

In another embodiment, the hydroxyl protecting group PG is a (heterocyclo)alkyl group. Suitable (heterocyclo)alkyl groups include, for example, 4-morpholinyl(C₁₋₄)alkyl groups, such as, 2-(4-morpholinyl)ethyl.

In another embodiment, the hydroxyl protecting group PG is a silyl group. The term “silyl” as employed herein refers to the group having the following structure:

wherein R¹⁷, R¹⁸, and R¹⁹ are each independently selected from the group consisting of alkyl, cycloalkyl, aryl, (cycloalkyl)alkyl, or arylalkyl, any of which is optionally substituted. In one embodiment, the silyl group is trimethyl silyl, tert-butyldimethyl silyl, tert-butyldiphenyl silyl, or tri-isopropyl silyl.

In another embodiment, the hydroxyl protecting group PG is an acyl group. The term “acyl” as employed herein refers to the following structure:

wherein R²⁰ is alkyl, cycloalkyl, aryl, (cycloalkyl)alkyl, or arylalkyl, any of which is optionally substituted. The acyl group can be, for example, C₁₋₄ alkylcarbonyl (such as, for example, acetyl), arylcarbonyl (such as, for example, benzoyl), levulinoyl, or pivaloyl. In another embodiment, the acyl group is benzoyl.

In another embodiment, the hydroxyl protecting group is a carbonate group. The term “carbonate” as employed herein refers to the following structure:

wherein R²¹ is alkyl, alkenyl, cycloalkyl, aryl, (cycloalkyl)alkyl, or arylalkyl, any of which is optionally substituted. Typically, R²¹ is C₁₋₁₀ alkyl (e.g., 2,4-dimethylpent-3-yl), C₂₋₆ alkenyl (e.g., ethenyl or prop-2-enyl, i.e., allyl), C₃₋₁₂ cycloalkyl (e.g., adamantyl), phenyl, or benzyl. In one embodiment, the carbonate is benzyloxycarbonyl.

The term “amine protecting group” as used herein refers to a group that blocks (i.e., protects) the amine functionality while reactions are carried out on other functional groups or parts of the molecule. Those skilled in the art will be familiar with the selection, attachment, and cleavage of amine protecting groups and will appreciate that many different protective groups are known in the art, the suitability of one protective group or another being dependent on the particular synthetic scheme planned. Treatises on the subject are available for consultation, such as Wuts, P. G. M. & Greene, T. W., Greene's Protective Groups in Organic Synthesis, 4rd Ed. (J. Wiley & Sons, 2007), herein incorporated by reference in its entirety. Suitable amine protecting groups include —CH₂—O—(CH₂)₂—Si(CH₃)₃, carbobenzyloxy (Cbz), tert-butyloxycarbonyl (BOC), 9-fluorenylmethyloxycarbonyl (FMOC), benzoyl (Bz), acetyl (Ac), carbamate, tosyl (Ts), and benzyl (Bn) groups.

Optional substituents attached to aryl, phenyl and heteroaryl rings each take the place of a hydrogen atom that would otherwise be present in any position on the aryl, phenyl or heteroaryl rings.

Useful halo or halogen groups include fluorine, chlorine, bromine and iodine.

Useful alkyl groups are selected from straight-chain and branched-chain C₁₋₁₀ alkyl groups. Typical C₁₋₁₀ alkyl groups include methyl (Me), ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl, and n-decyl, isopropyl, sec-butyl, tert-butyl, iso-butyl, iso-pentyl, neopentyl, 1-methylbutyl, 2-methylbutyl, 3-methylbutyl, 1,1-dimethylpropyl, 1,2-dimethylpropyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 3-ethylbutyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 1,3-dimethylbutyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl, 3,3-dimethylbutyl, 1-methylhexyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 1,2-dimethylpentyl, 1,3-dimethylpentyl, 1,2-dimethylhexyl, 1,3-dimethylhexyl, 3,3-dimethylhexyl, 1,2-dimethylheptyl, 1,3-dimethylheptyl, and 3,3-dimethylheptyl, among others. In one embodiment, useful alkyl groups are selected from straight chain C₁₋₆ alkyl groups and branched chain C₃₋₆ alkyl groups. Typical C₁₋₆ alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, iso-butyl, pentyl, 3-pentyl, hexyl, among others. In one embodiment, useful alkyl groups are selected from straight chain C₂₋₆ alkyl groups and branched chain C₃₋₆ alkyl groups. Typical C₂₋₆ alkyl groups include ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, iso-butyl, pentyl, 3-pentyl, hexyl among others. In one embodiment, useful alkyl groups are selected from straight chain C₁₋₄ alkyl groups and branched chain C₃₋₄ alkyl groups. Typical C₁₋₄ alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tert-butyl, and iso-butyl.

Useful alkenyl groups are selected from straight-chain and branched-chain C₂₋₆ alkenyl groups, preferably C₂₋₄ alkenyl. Typical C₂₋₆ alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, sec-butenyl, pentenyl, and hexenyl. Typical C₂₋₄ alkenyl groups include ethenyl, propenyl, isopropenyl, butenyl, and sec-butenyl.

Useful alkynyl groups are selected from straight-chain and branched-chain C₂₋₆ alkynyl groups, preferably C₂₋₄ alkynyl. Typical C₂₋₆ alkynyl groups include ethynyl, propynyl, butynyl, 2-butynyl, pentynyl, and hexynyl groups. Typical C₂₋₄ alkynyl groups include ethynyl, propynyl, butynyl, and 2-butynyl groups.

Useful haloalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, preferably any of the above-mentioned C₁₋₆ alkyl groups, and preferably any of the above-mentioned C₁₋₄ alkyl groups, substituted by one or more fluorine, chlorine, bromine or iodine atoms (e.g., fluoromethyl, difluoromethyl, trifluoromethyl, pentafluoroethyl, 1,1-difluoroethyl, 2,2-difluoroethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl, 4,4,4-trifluorobutyl, and trichloromethyl groups).

Useful hydroxyalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, preferably any of the above-mentioned C₁₋₆ alkyl groups, and preferably any of the above-mentioned C₁₋₄ alkyl groups, substituted by one or more hydroxy groups, such as monohydroxyalkyl and dihydroxyalkyl groups (e.g., hydroxymethyl, hydroxyethyl, hydroxypropyl, hydroxybutyl, hydroxypentyl, and hydroxyhexyl groups, and especially hydroxymethyl, 1-hydroxyethyl, 2-hydroxyethyl, 1,2-dihydroxyethyl, 2-hydroxypropyl, 2-hydroxyprop-2-yl, 3-hydroxypropyl, 2,3-dihydroxypropyl, 3-hydroxybutyl, 4-hydroxybutyl, 2-hydroxy-1-methylpropyl, and 1,3-dihydroxyprop-2-yl). In one embodiment, the monohydroxyalkyl is monohydroxy(C₁₋₄ alkyl. In one embodiment, the dihydroxyalkyl is dihydroxy(C₁₋₄ alkyl.

Useful cycloalkyl groups, as used by itself or as part of another group, are selected from saturated cyclic hydrocarbon groups containing 1, 2, or 3 rings having 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12 carbon atoms (i.e., C₃-C₁₂ cycloalkyl) or the number of carbons designated. In one embodiment, the cycloalkyl has one or two rings. In another embodiment, the cycloalkyl is a C₃-C₈ cycloalkyl. In another embodiment, the cycloalkyl is C₃₋₇ cycloalkyl. In another embodiment, the cycloalkyl is C₃₋₆ cycloalkyl. Exemplary cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, norbornyl, decalin, and adamantyl.

Useful cycloalkenyl groups, as used by itself or as part of another group, are selected from partially unsaturated (i.e., containing one or two double bonds) cyclic hydrocarbon groups containing 1, 2, or 3 rings having 4, 5, 6, 7, 8, 9, 10, 11, or 12 carbon atoms (i.e., C₄-C₁₂ cycloalkenyl) or the number of carbons designated. In one embodiment, the cycloalkenyl has one or two rings. In another embodiment, the cycloalkenyl is a C₃-C₈ cycloalkenyl. In another embodiment, the cycloalkenyl is C₃₋₇ cycloalkenyl. In another embodiment, the cycloalkenyl is C₃₋₆ cycloalkenyl. In one embodiment, the cycloalkenyl group contains one double bond. Exemplary cycloalkenyl groups containing one double bond include cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl, cyclononenyl, and cyclodecenyl. In another embodiment, the cycloalkenyl group contains two double bonds. Preferably, the cycloalkenyl groups containing two double bonds have 5, 6, 7, 8, 9, 10, 1, or 12 carbon atoms (i.e., C₅-C₁₂ cycloalkadienyl). Exemplary cycloalkenyl groups having two double bonds include cyclopentadienyl, cyclohexadienyl, cycloheptadienyl, cyclooctadienyl, cyclononadienyl, and cyclodecadienyl.

Useful alkoxy groups include oxygen substituted by one of the C₁₋₁₀ alkyl groups mentioned above (e.g., methoxy, ethoxy, propoxy, iso-propoxy, butoxy, tert-butoxy, iso-butoxy, sec-butoxy, pentyloxy, hexyloxy, heptyloxy, octyloxy, nonyloxy and decyloxy), preferably by one of the C₁₋₆ alkyl groups, and more preferably by one of the C₁₋₄ alkyl groups.

Useful alkenyloxy groups include oxygen substituted by one of the C₂₋₆ alkenyl groups, and preferably by one of the C₂₋₄ alkenyl groups, mentioned above (e.g., ethenyloxy, propenyloxy, isopropenyloxy, butenyloxy, sec-butenyloxy, pentenyloxy, and hexenyloxy).

Useful alkynyloxy groups include oxygen substituted by one of the C₂₋₆ alkynyl groups, preferably by one of the C₂₋₄ alkynyl groups, mentioned above (e.g., ethynyloxy, propynyloxy, butynyloxy, 2-butynyloxy, pentynyloxy, and hexynyloxy).

Useful alkoxyalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned alkoxy groups (e.g., methoxymethyl, methoxyethyl, methoxypropyl, methoxybutyl, ethoxymethyl, 2-ethoxyethyl, 3-ethoxypropyl, 4-ethoxybutyl, propoxymethyl, iso-propoxymethyl, 2-propoxyethyl, 3-propoxypropyl, butoxymethyl, tert-butoxymethyl, isobutoxymethyl, sec-butoxymethyl, and pentyloxymethyl).

Useful haloalkoxy groups include oxygen substituted by one of the C₁₋₁₀ haloalkyl groups, and preferably one of the C₁₋₆ haloalkyl groups, mentioned above (e.g., fluoromethoxy, difluoromethoxy, trifluoromethoxy, and 2,2,2-trifluoroethoxy).

The term “(cycloalkyl)alkyl” as used by itself or as part of another group refers to any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned cycloalkyl groups (e.g., (cyclopropyl)methyl, 2-(cyclopropyl)ethyl, (cyclopropyl)propyl, (cyclobutyl)methyl, (cyclopentyl)methyl, and (cyclohexyl)methyl).

The term “(cycloalkenyl)alkyl” as used by itself or as part of another group refers to any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned cycloalkenyl groups (e.g., (cyclobutenyl)methyl, 2-(cyclobutenyl)ethyl, (cyclobutenyl)propyl, (cyclopentenyl)methyl, (cyclohexenyl)methyl, and (cyclopentadienyl)methyl).

Useful aryl groups, as used by itself or as part of another group, are C₆₋₁₄ aryl, especially C₆₋₁₀ aryl. Typical C₆₋₁₄ aryl groups include phenyl (Ph), naphthyl, phenanthryl, anthracyl, indenyl, azulenyl, biphenyl, biphenylenyl, and fluorenyl groups, more preferably phenyl, naphthyl, and biphenyl groups.

Useful aryloxy groups include oxygen substituted by one of the aryl groups mentioned above (e.g., phenoxy).

The term “arylalkyl” as used by itself or as part of another group refers to any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted by any of the above-mentioned aryl groups (e.g., benzyl and phenethyl).

Useful arylalkenyl groups include any of the above-mentioned C₂₋₆ alkenyl groups substituted by any of the above-mentioned aryl groups (e.g., phenylethenyl).

Useful arylalkynyl groups include any of the above-mentioned C₂₋₆ alkynyl groups substituted by any of the above-mentioned aryl groups (e.g., phenylethynyl).

Useful aralkyloxy or arylalkoxy groups include oxygen substituted by one of the above-mentioned arylalkyl groups (e.g., benzyloxy).

Useful (arylalkoxy)carbonyl groups include a carbonyl group substituted by any of the above-mentioned arylalkoxy groups (e.g., (benzyloxy)carbonyl).

The term “heteroaryl” or “heteroaromatic” as employed herein by itself or as part of another group refers to groups having 5 to 14 ring atoms, with 6, 10 or 14 π electrons shared in a cyclic array, and containing carbon atoms and 1, 2, or 3 oxygen, nitrogen or sulfur heteroatoms, or 4 nitrogen atoms. In one embodiment, the heteroaryl group is a 5- to 10-membered heteroaryl group. Examples of heteroaryl groups include thienyl, benzo[b]thienyl, naphtho[2,3-b]thienyl, thianthrenyl, furyl, benzofuryl, pyranyl, isobenzofuranyl, benzooxazonyl, chromenyl, xanthenyl, 2H-pyrrolyl, pyrrolyl, imidazolyl, pyrazolyl, pyridyl, pyrazinyl, pyrimidinyl, pyridazinyl, isoindolyl, 3H-indolyl, indolyl, indazolyl, purinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, cinnolinyl, quinazolinyl, pteridinyl, 4aH-carbazolyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, pyrimidinyl, phenanthrolinyl, phenazinyl, thiazolyl, isothiazolyl, phenothiazolyl, isoxazolyl, furazanyl, and phenoxazinyl. Typical heteroaryl groups include thienyl (e.g., thien-2-yl and thien-3-yl), furyl (e.g., 2-furyl and 3-furyl), pyrrolyl (e.g., pyrrol-1-yl, 1H-pyrrol-2-yl and 1H-pyrrol-3-yl), imidazolyl (e.g., imidazol-1-yl, 1H-imidazol-2-yl and 1H-imidazol-4-yl), tetrazolyl (e.g., tetrazol-1-yl and tetrazol-5-yl), pyrazolyl (e.g., 1H-pyrazol-3-yl, 1H-pyrazol-4-yl, and 1H-pyrazol-5-yl), pyridyl (e.g., pyridin-2-yl, pyridin-3-yl, and pyridin-4-yl), pyrimidinyl (e.g., pyrimidin-2-yl, pyrimidin-4-yl, pyrimidin-5-yl, and pyrimidin-5-yl), thiazolyl (e.g., thiazol-2-yl, thiazol-4-yl, and thiazol-5-yl), isothiazolyl (e.g., isothiazol-3-yl, isothiazol-4-yl, and isothiazol-5-yl), oxazolyl (e.g., oxazol-2-yl, oxazol-4-yl, and oxazol-5-yl) and isoxazolyl (e.g., isoxazol-3-yl, isoxazol-4-yl, and isoxazol-5-yl). A 5-membered heteroaryl can contain up to 4 heteroatoms. A 6-membered heteroaryl can contain up to 3 heteroatoms. Each heteroatom is independently selected from nitrogen, oxygen and sulfur.

The term “heteroarylalkyl” as used by itself or as part of another group refers to any of the above-mentioned C₁₋₁₀ alkyl groups substituted by any of the above-mentioned heteroaryl groups (e.g., (thien-2-yl)methyl, 2-furylmethyl, (pyrrol-1-yl)methyl, and 2-(1H-pyrrol-2-yl)ethyl).

Useful heteroarylalkoxy groups include oxygen substituted by one of the above-mentioned heteroaryl groups.

Useful (heteroarylalkoxy)carbonyl groups include a carbonyl group substituted by any of the above-mentioned heteroarylalkoxy groups.

The terms “heterocyclic” and “heterocyclo” as used by itself or as part of another group mean saturated or partially unsaturated 3-7 membered monocyclic, or 7-10 membered bicyclic ring system, which consist of carbon atoms and from one to four heteroatoms independently selected from the group consisting of O, N, and S, wherein the nitrogen and sulfur heteroatoms can be optionally oxidized, the nitrogen can be optionally quaternized, and including any bicyclic group in which any of the above-defined heterocyclic rings is fused to a benzene ring, and wherein the heterocyclic ring can be substituted on a carbon atom or on a nitrogen atom if the resulting compound is stable. In one embodiment, the 3- to 7-membered monocyclic heterocyclic ring is either a saturated, or unsaturated non-aromatic ring. A 3-membered heterocyclo can contain up to 1 heteroatom, a 4-membered heterocyclo can contain up to 2 heteroatoms, a 5-membered heterocyclo can contain up to 4 heteroatoms, a 6-membered heterocyclo can contain up to 4 heteroatoms, and a 7-membered heterocyclo can contain up to 5 heteroatoms. Each heteroatom is independently selected from nitrogen, which can be quaternized; oxygen; and sulfur, including sulfoxide and sulfone. The 3- to 7-membered heterocyclo can be attached via a nitrogen or carbon atom. A 7- to 10-membered bicyclic heterocyclo contains from 1 to 4 heteroatoms independently selected from nitrogen, which can be quaternized; oxygen; and sulfur, including sulfoxide and sulfone. The 7- to 10-membered bicyclic heterocyclo can be attached via a nitrogen or carbon atom. Examples of the heterocyclic rings include, but are not limited to, pyrrolidinyl, pyrrolidinonyl, piperidinyl, piperazinyl, morpholinyl, thiomorpholinyl, imidazolinyl, pyrazolidinyl, tetrahydrofuranyl, oxazolidinyl, 2-oxooxazolidinyl, tetrahydrothienyl, imidazolidinyl, hexahydropyrimidinyl, 2,3-dihydrofuranyl, dihydropyranyl, hydantoinyl, valerolactamyl, oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, dihydropyridinyl, tetrahydropyridinyl, tetrahydropyrimidinyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, and benzodiazepines.

The term “(heterocyclo)alkyl” as used by itself or as part of another group refers to any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted by any of the above-mentioned heterocyclic groups (e.g., (pyrrolidin-2-yl)methyl, (pyrrolidin-1-yl)methyl, (piperidin-1-yl)methyl, (morpholin-4-yl)methyl, (2-oxooxazolidin-4-yl)methyl, 2-(2-oxooxazolidin-4-yl)ethyl, (2-oxo-imidazolidin-1-yl)methyl, (2-oxo-imidazolidin-1-yl)ethyl, and (2-oxo-imidazolidin-1-yl)propyl).

As used herein, the term “amino” or “amino group” refers to —NH₂.

Useful aminoalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with one or more amino group.

Useful alkylamino and dialkylamino groups are —NHR²² and —NR²²R²³, respectively, wherein R²² and R²³ are each independently selected from a C₁₋₁₀ alkyl group.

Useful (alkylamino)alkyl and (dialkylamino)alkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned alkylamino and dialkylamino groups, respectively.

As used herein, the term “aminocarbonyl” refers to —C(═O)NH₂.

Useful alkylcarbonyl groups include a carbonyl group, i.e., —C(═O)—, substituted by any of the above-mentioned C₁₋₁₀ alkyl groups.

Useful alkoxycarbonyl groups include a carbonyl group substituted by any of the above-mentioned alkoxy groups (e.g., methoxycarbonyl, ethoxycarbonyl, propoxycarbonyl, iso-propoxycarbonyl, butoxycarbonyl, tert-butoxycarbonyl, iso-butoxycarbonyl, sec-butoxycarbonyl, and pentyloxycarbonyl).

Useful (alkoxycarbonyl)alkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned alkoxycarbonyl group.

Useful arylcarbonyl groups include a carbonyl group substituted by any of the above-mentioned aryl groups (e.g., benzoyl).

Useful alkylcarbonyloxy or acyloxy groups include oxygen substituted by one of the above-mentioned alkylcarbonyl groups.

Useful alkylcarbonylamino or acylamino groups include any of the above-mentioned alkylcarbonyl groups attached to an amino nitrogen, such as methylcarbonylamino.

As used herein, the term “carboxamido” refers to a radical of formula —C(═O)NR²⁴R²⁵, wherein R²⁴ and R²⁵ are each independently hydrogen, optionally substituted C₁₋₁₀ alkyl, or optionally substituted aryl. Exemplary carboxamido groups include —CONH₂, —CON(H)CH₃, —CON(CH₃)₂, and —CON(H)Ph.

Useful (aminocarbonyl)alkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with one or more of the above-mentioned aminocarbonyl groups.

Useful alkylaminocarbonyl and dialkylaminocarbonyl groups are any of the above-mentioned carboxamido groups, where R²⁴ is H and R²⁵ is C₁₋₁₀ alkyl or where R²⁴ and R²⁵ are each independently selected from a C₁₋₁₀ alkyl group, respectively.

Useful (alkylaminocarbonyl)alkyl and (dialkylaminocarbonyl)alkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted with any of the above-mentioned alkylaminocarbonyl and dialkylaminocarbonyl groups, respectively.

As used herein, the term “sulfonamido” refers to a radical of formula —SO₂NR²⁶R²⁷, wherein R²⁶ and R²⁷ are each independently hydrogen, optionally substituted C₁₋₁₀ alkyl, or optionally substituted aryl. Exemplary sulfonamido groups include —SO₂NH₂, —SO₂N(H)CH₃, and —SO₂N(H)Ph.

As used herein, the term “thiol” refers to —SH.

Useful mercaptoalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted by a —SH group.

As used herein, the term “carboxy” refers to —COOH.

Useful carboxyalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted by —COOH.

As used herein, the terms “hydroxyl” or “hydroxy” refer to —OH.

As used herein, the term “cyano” refers to —CN.

As used herein, the term “nitro” refers to —NO₂.

As used herein, the term “ureido” refers to —NH—C(═O)—NH₂.

As used herein, the term “azido” refers to —N₃.

As used herein, the term “guanidino” refers to —NH—C(═NH)—NH₂.

Useful guanidinoalkyl groups include any of the above-mentioned C₁₋₁₀ alkyl groups, and preferably any of the above-mentioned C₁₋₆ alkyl groups, substituted by —NH—C(═NH)—NH₂.

The term “ambient temperature” as used herein means the temperature of the surroundings. The ambient temperature indoors is the same as room temperature, which is from about 20° C. to about 25° C.

The term “about,” as used herein in connection with a measured quantity, refers to the normal variations in that measured quantity, as expected by the skilled artisan making the measurement and exercising a level of care commensurate with the objective of measurement and the precision of the measuring equipment. Typically, the term “about” includes the recited number ±10%. Thus, “about 10” means 9 to 11.

As used herein, the term “optionally substituted” refers to a group that may be unsubstituted or substituted.

The term “portion(s)” or the phrase “portion(s) thereof” as used herein in connection with, for example, the phrases “the cycloalkyl, cycloalkenyl, heterocyclo, aryl and heteroaryl portions are optionally substituted” or “said cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions thereof are optionally substituted”, respectively, refers to the cyclic moieties s (i.e., cycloalkyl, cycloalkenyl, heterocyclo, aryl and heteroaryl), of groups such as, for example, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, and heteroarylalkyl.

Optional substituents on optionally substituted groups, when not otherwise indicated, include one or more groups, typically 1, 2, or 3 groups, independently selected from the group consisting of halo, halo(C₁₋₆)alkyl, aryl, heterocycle, cycloalkyl, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, aryl(C₁₋₆)alkyl, aryl(C₂₋₆)alkenyl, aryl(C₂₋₆)alkynyl, cycloalkyl(C₁₋₆)alkyl, heterocyclo(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, amino(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, alkoxy(C₁₋₆)alkyl, nitro, amino, ureido, cyano, alkylcarbonylamino, hydroxy, thiol, alkylcarbonyloxy, aryloxy, ar(C₁₋₆)alkyloxy, carboxamido, sulfonamido, azido, C₁₋₆ alkoxy, halo(C₁₋₆)alkoxy, carboxy, aminocarbonyl, (═O), and mercapto(C₁₋₆)alkyl groups mentioned above. Preferred optional substituents include halo, halo(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, amino(C₁₋₆)alkyl, hydroxy, nitro, C₁₋₆ alkyl, C₁₋₆ alkoxy, halo(C₁₋₆)alkoxy, and amino.

Compounds of the Invention encompass all the salts of the disclosed compounds of Formulae I, IA, and II-XVI. The present invention preferably includes all non-toxic pharmaceutically acceptable salts thereof of the disclosed compounds. Examples of pharmaceutically acceptable addition salts include inorganic and organic acid addition salts and basic salts. The pharmaceutically acceptable salts include, but are not limited to, metal salts such as sodium salt, potassium salt, cesium salt and the like; alkaline earth metals such as calcium salt, magnesium salt and the like; organic amine salts such as triethylamine salt, pyridine salt, picoline salt, ethanolamine salt, triethanolamine salt, dicyclohexylamine salt, N,N′-dibenzylethylenediamine salt and the like; inorganic acid salts such as hydrochloride, hydrobromide, phosphate, sulphate and the like; organic acid salts such as citrate, lactate, tartrate, maleate, fumarate, mandelate, acetate, dichloroacetate, trifluoroacetate, oxalate, formate and the like; sulfonates such as methanesulfonate, benzenesulfonate, p-toluenesulfonate and the like; and amino acid salts such as arginate, asparginate, glutamate and the like.

Acid addition salts can be formed by mixing a solution of the particular compound of the present invention with a solution of a pharmaceutically acceptable non-toxic acid such as hydrochloric acid, fumaric acid, maleic acid, succinic acid, acetic acid, citric acid, tartaric acid, carbonic acid, phosphoric acid, oxalic acid, dichloroacetic acid, or the like. Basic salts can be formed by mixing a solution of the compound of the present invention with a solution of a pharmaceutically acceptable non-toxic base such as sodium hydroxide, potassium hydroxide, choline hydroxide, sodium carbonate and the like.

Compounds of the Invention also encompass solvates of any of the disclosed compounds of Formulae I, IA, and II-XVI. Solvates typically do not significantly alter the physiological activity or toxicity of the compounds, and as such may function as pharmacological equivalents. The term “solvate” as used herein is a combination, physical association and/or solvation of a compound of the present invention with a solvent molecule such as, e.g. a disolvate, monosolvate or hemisolvate, where the ratio of solvent molecule to compound of the present invention is about 2:1, about 1:1 or about 1:2, respectively. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate can be isolated, such as when one or more solvent molecules are incorporated into the crystal lattice of a crystalline solid. Thus, “solvate” encompasses both solution-phase and isolatable solvates. Compounds of the Invention may be present as solvated forms with a pharmaceutically acceptable solvent, such as water, methanol, ethanol, and the like, and it is intended that the invention includes both solvated and unsolvated forms of compounds of any of Formulae I, IA, and II-XVI. One type of solvate is a hydrate. A “hydrate” relates to a particular subgroup of solvates where the solvent molecule is water. Solvates typically can function as pharmacological equivalents. Preparation of solvates is known in the art. See, for example, M. Caira et al., J. Pharmaceut. Sci., 93(3):601-611 (2004), which describes the preparation of solvates of fluconazole with ethyl acetate and with water. Similar preparation of solvates, hemisolvates, hydrates, and the like are described by E. C. van Tonder et al., AAPS Pharm. Sci. Tech., 5(1):Article 12 (2004), and A. L. Bingham et al., Chem. Commun.: 603-604 (2001). A typical, non-limiting, process of preparing a solvate would involve dissolving a compound of any of Formulae I, IA, and II-XVI in a desired solvent (organic, water, or a mixture thereof) at temperatures above about 20° C. to about 25° C., then cooling the solution at a rate sufficient to form crystals, and isolating the crystals by known methods, e.g., filtration. Analytical techniques such as infrared spectroscopy can be used to confirm the presence of the solvent in a crystal of the solvate.

Compounds of the Invention can be isotopically-labeled (i.e., radio-labeled). Examples of isotopes that can be incorporated into the disclosed compounds include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as ²H, ³H, ¹¹C, ¹³C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ³¹P, ³²P, ³⁵S, ¹⁸F and ³⁶Cl, respectively, and preferably ³H, ¹¹C, and ¹⁴C. Isotopically-labeled Compounds of the Invention can be prepared by methods known in the art in view of this disclosure. For example, tritiated Compounds of the Invention can be prepared by introducing tritium into the particular compound by catalytic dehalogenation with tritium. This method may include reacting a suitable halogen-substituted precursor of a Compound of the Invention with tritium gas in the presence of an appropriate catalyst such as Pd/C in the presence of a base. Other suitable methods for preparing tritiated compounds can be found in Filer, Isotopes in the Physical and Biomedical Sciences, Vol. 1, Labeled Compounds (Part A), Chapter 6 (1987). ¹⁴C-labeled compounds can be prepared by employing starting materials having a ¹⁴C carbon.

Isotopically labeled Compounds of the Invention, as well as the pharmaceutically acceptable salts and solvates thereof, can be used as radioligands to test for the binding of compounds to an opioid receptor. For example, a radio-labeled Compound of the Invention can be used to characterize specific binding of a test or candidate compound to the receptor. Binding assays utilizing such radio-labeled compounds can provide an in vitro alternative to animal testing for the evaluation of chemical structure-activity relationships. For example, the receptor assay may be performed at a fixed concentration of a radiolabeled Compound of the Invention and at increasing concentrations of a test compound in a competition assay. In a non-limiting embodiment, the present invention provides a method for screening a candidate compound for the ability to bind to an opioid receptor, comprising a) introducing a fixed concentration of a radio-labeled Compound of the Invention to the receptor under conditions that permit binding of the radio-labeled compound to the receptor to form a complex; b) titrating the complex with a candidate compound; and c) determining the binding of the candidate compound to said receptor.

Some of the compounds disclosed herein may contain one or more asymmetric centers and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms, such as epimers. The present invention is meant to encompass the uses of all such possible forms, as well as their racemic and resolved forms and mixtures thereof. The individual enantiomers may be separated according to methods known to those of ordinary skill in the art in view of the present disclosure. When the compounds described herein contain olefinic double bonds or other centers of geometric asymmetry, and unless specified otherwise, it is intended that they include both E and Z geometric isomers. All tautomers are intended to be encompassed by the present invention as well.

As used herein, the term “stereoisomers” is a general term for all isomers of individual molecules that differ only in the orientation of their atoms in space. It includes enantiomers and isomers of compounds with more than one chiral center that are not mirror images of one another (diastereomers).

The term “chiral center” refers to a carbon atom to which four different groups are attached.

The term “epimer” refers to diastereomers that have opposite configuration at only one of two or more tetrahedral streogenic centres present in the respective molecular entities.

The term “stereogenic center” is an atom, bearing groups such that an interchanging of any two groups leads to a stereoisomer.

The terms “enantiomer” and “enantiomeric” refer to a molecule that cannot be superimposed on its mirror image and hence is optically active wherein the enantiomer rotates the plane of polarized light in one direction and its mirror image compound rotates the plane of polarized light in the opposite direction.

The term “racemic” refers to a mixture of equal parts of enantiomers and which mixture is optically inactive.

The term “resolution” refers to the separation or concentration or depletion of one of the two enantiomeric forms of a molecule.

The terms “a” and “an” refer to one or more.

The term “treating” or “treatment” refers to administering a therapy in an amount, manner, or mode effective to improve a condition, symptom, or parameter associated with a disorder or to prevent progression of a disorder, to either a statistically significant degree or to a degree detectable to one skilled in the art. An effective amount, manner, or mode can vary depending on the subject and may be tailored to the patient.

Open terms such as “include,” “including,” “contain,” “containing” and the like mean “comprising.”

As used herein, compounds that bind to receptors and mimic the regulatory effects of endogenous ligands are defined as “agonists”. Compounds that bind to receptors and are only partly effective as agonists are defined as “partial agonists”. Compounds that bind to a receptor but produce no regulatory effect, but rather block the binding of ligands to the receptor are defined as “antagonists”. (Ross and Kenakin, “Ch. 2: Pharmacodynamics: Mechanisms of Drug Action and the Relationship Between Drug Concentration and Effect”, pp. 31-32, in Goodman & Gilman's the Pharmacological Basis of Therapeutics, 10^(th) Ed. (J. G. Hardman, L. E. Limbird and A. Goodman-Gilman eds., 2001)).

In certain embodiments, the Compound of the Invention is an agonist at one or more of the μ, δ and/or κ opioid receptors. In certain non-limiting embodiments, the Compound of the Invention produces fewer side effects and/or less severe side effects than currently available analgesic opioid compounds when administered at doses producing equivalent levels of analgesia and/or anti-hyperalgesia. In certain embodiments, the Compound of the Invention is an agonist at ORL-1 opioid receptor.

In certain embodiments, Compounds of the Invention can be used in combination with at least one other therapeutic agent. The other therapeutic agent can be, but is not limited to, a μ-opioid agonist, a non-opioid analgesic, a non-steroidal anti-inflammatory agent, a Cox-II inhibitor, an anti-emetic, a β-adrenergic blocker, an anticonvulsant, an antidepressant, a Ca²⁺-channel blocker, an anticancer agent, or a mixture thereof.

Compounds of the Invention potently bind to the μ and/or κ and/or δ and/or ORL-1 opioid receptors. Compounds of the Invention can be modulators at the μ and/or κ and/or δ and/or ORL-1 opioid receptors, and therefore Compounds of the Invention can be used/administered to treat, ameliorate, or prevent pain.

In some embodiments, Compounds of the Invention are antagonists of one or more opioid receptors. In another embodiment, Compounds of the Invention are antagonists of the μ and/or κ opioid receptors.

In some embodiments, Compounds of the Invention are partial agonists of one or more opioid receptors. In another embodiment, Compounds of the Invention are partial agonists of the μ and/or κ opioid receptors.

In another embodiment, Compounds of the Invention are agonists of one or more opioid receptors. In another embodiment, Compounds of the Invention are agonists of the μ and/or κ opioid receptors.

In some embodiments, Compounds of the Invention have both: (i) antagonist activity at the ORL-1 receptor; and (ii) agonist activity at one or more of the μ, δ and/or κ receptors. In another embodiment, Compounds of the Invention have both: (i) antagonist activity at the ORL-1 receptor; and (ii) agonist activity at the μ receptor. In another embodiment, Compounds of the Invention have both: (i) antagonist activity at the μ receptor; and (ii) agonist activity at the κ receptor. In another embodiment, Compounds of the Invention have: (i) antagonist activity at the ORL-1 receptor; (ii) antagonist activity at the μ receptor; and (iii) agonist activity at the κ receptor. In another embodiment, Compounds of the Invention have: (i) antagonist activity at the μ receptor; (ii) agonist activity at the κ receptor; and (iii) antagonist activity at the δ receptor.

Compounds of the Invention that are antagonists of the μ-opioid receptor or agonists of κ-opioid receptor, or both, can be used/administered to treat or ameliorate constipation. Compounds of the Invention that are agonists of μ-opioid receptor can be used/administered to treat or ameliorate diarrhea.

Compounds of the Invention can be used to treat or prevent acute, chronic pain (which includes but is not limited to, neuropathic pain, postoperative pain, and inflammatory pain), or surgical pain. Examples of pain that can be treated or prevented using a Compound of the Invention include, but are not limited to, cancer pain, neuropathic pain, labor pain, myocardial infarction pain, pancreatic pain, colic pain, post-operative pain, headache pain, muscle pain, arthritic pain, and pain associated with a periodontal disease, including gingivitis and periodontitis.

Acute pain includes, but is not limited to, perioperative pain, postoperative pain, post-traumatic pain, acute disease related pain, and pain related to diagnostic procedures, orthopedic manipulations, and myocardial infarction. Acute pain in the perioperative setting includes pain because of pre-existing disease, the surgical procedure, e.g., associated drains, chest or nasogastric tubes, or complications, or a combination of disease-related and procedure-related sources.

Chronic pain includes, but is not limited to, inflammatory pain, postoperative pain, cancer pain, osteoarthritis pain associated with metastatic cancer, trigeminal neuralgia, acute herpetic and postherpetic neuralgia, diabetic neuropathy, causalgia, brachial plexus avulsion, occipital neuralgia, reflex sympathetic dystrophy, fibromyalgia, gout, phantom limb pain, burn pain, and other forms of neuralgia, neuropathic, and idiopathic pain syndromes.

Compounds of the Invention can be used to treat or prevent pain associated with inflammation or with an inflammatory disease in a patient. Such pain can arise where there is an inflammation of the body tissue which can be a local inflammatory response or a systemic inflammation. For example, a Compound of the Invention can be used to treat or prevent pain associated with inflammatory diseases including, but not limited to, organ transplant rejection; reoxygenation injury resulting from organ transplantation (see Grupp et al., J. Mol, Cell Cardiol. 31:297-303 (1999)) including, but not limited to, transplantation of the heart, lung, liver, or kidney; chronic inflammatory diseases of the joints, including arthritis, rheumatoid arthritis, osteoarthritis and bone diseases associated with increased bone resorption; inflammatory bowel diseases, such as ileitis, ulcerative colitis, Barrett's syndrome, and Crohn's disease; inflammatory lung diseases, such as asthma, adult respiratory distress syndrome, and chronic obstructive airway disease; inflammatory diseases of the eye, including corneal dystrophy, trachoma, onchocerciasis, uveitis, sympathetic ophthalmitis and endophthalmitis; chronic inflammatory disease of the gum, including gingivitis and periodontitis; tuberculosis; leprosy; inflammatory diseases of the kidney, including uremic complications, glomerulonephritis and nephrosis; inflammatory disease of the skin, including sclerodermatitis, psoriasis and eczema; inflammatory diseases of the central nervous system, including chronic demyelinating diseases of the nervous system, multiple sclerosis, AIDS-related neurodegeneration and Alzheimer's disease, infectious meningitis, encephalomyelitis, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis and viral or autoimmune encephalitis; autoimmune diseases, including Type I and Type II diabetes mellitus; diabetic complications, including, but not limited to, diabetic cataract, glaucoma, retinopathy, nephropathy (such as microaluminuria and progressive diabetic nephropathy), gangrene of the feet, atherosclerotic coronary arterial disease, peripheral arterial disease, nonketotic hyperglycemic-hyperosmolar coma, foot ulcers, joint problems, and a skin or mucous membrane complication (such as an infection, a shin spot, a candidal infection or necrobiosis lipoidica diabeticorum), immune-complex vasculitis, and systemic lupus erythematosus (SLE); inflammatory disease of the heart, such as cardiomyopathy, ischemic heart disease hypercholesterolemia, and artherosclerosis; as well as various other diseases that can have significant inflammatory components, including preeclampsia, chronic liver failure, brain and spinal cord trauma, and cancer. Compounds of the Invention can also be used to treat or prevent pain associated with inflammatory disease that can, for example, be a systemic inflammation of the body, exemplified by gram-positive or gram negative shock, hemorrhagic or anaphylactic shock, or shock induced by cancer chemotherapy in response to pro-inflammatory cytokines, e.g., shock associated with pro-inflammatory cytokines. Such shock can be induced, e.g., by a chemotherapeutic agent that is administered as a treatment for cancer.

Compounds of the Invention can be used to treat or prevent pain associated with nerve injury (i.e., neuropathic pain). Chronic neuropathic pain is a heterogenous disease state with an unclear etiology. In chronic pain, the pain can be mediated by multiple mechanisms. This type of pain generally arises from injury to the peripheral or central nervous tissue. The syndromes include pain associated with spinal cord injury, multiple sclerosis, post-herpetic neuralgia, trigeminal neuralgia, phantom pain, causalgia, and reflex sympathetic dystrophy and lower back pain. The chronic pain is different from acute pain in that chronic neuropathic pain patients suffer the abnormal pain sensations that can be described as spontaneous pain, continuous superficial burning and/or deep aching pain. The pain can be evoked by heat-, cold-, and mechano-hyperalgesia or by heat-, cold-, or mechano-allodynia.

Chronic neuropathic pain can be caused by injury or infection of peripheral sensory nerves. It includes, but is not limited to pain from peripheral nerve trauma, herpes virus infection, diabetes mellitus, causalgia, plexus avulsion, neuroma, limb amputation, and vasculitis. Neuropathic pain can also be caused by nerve damage from chronic alcoholism, human immunodeficiency virus infection, hypothyroidism, uremia, or vitamin deficiences. Stroke (spinal or brain) and spinal cord injury can also induce neuropathic pain. Cancer-related neuropathic pain results from tumor growth compression of adjacent nerves, brain, or spinal cord. In addition, cancer treatments, including chemotherapy and radiation therapy, can cause nerve injury. Neuropathic pain includes but is not limited to pain caused by nerve injury such as, for example, the pain from which diabetics suffer.

Compounds of the Invention can be used to treat or prevent pain associated with migraine including, but not limited to, migraine without aura (“common migraine”), migraine with aura (“classic migraine”), migraine without headache, basilar migraine, familial hemiplegic migraine, migrainous infarction, and migraine with prolonged aura.

Compounds of the Invention can also be used as an agent to treat or prevent withdrawal from alcohol addiction or drug addiction; as an agent to treat or prevent addictive disorders; as an agent to treat a pruritic condition; and in treating or ameliorating constipation and diarrhea.

The present invention is also directed to the use of a compound represented by any of defined Formulae I, IA, and II-XVI, or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament for treating a disorder responsive to the modulation of one or more opioids receptors (e.g., any of the disorders listed above) in a patient suffering from said disorder.

Furthermore, the present invention is directed to a method of modulating, in particular activating, one or more opioid receptors in a patient in need thereof, said method comprising administering to the patient at least one compound represented by any of defined Formulae I, IA, and II-XVI, or a pharmaceutically acceptable salt or solvate thereof.

The present invention is also directed to the use of a compound represented by any of defined Formulae I, IA, and II-XVI, or a pharmaceutically acceptable salt or solvate thereof, in the manufacture of a medicament, in particular a medicament for modulating, in particular activating, one or more opioid receptors, in a patient in need thereof.

Synthesis of Compounds

Compounds of the Invention can be prepared using methods known to those skilled in the art in view of this disclosure, or by illustrative methods shown in the schemes below. For example, compounds of Formulae I, IA, and II-XVI can be prepared as shown in the Schemes A-F below. Additional methods of synthesis are described and illustrated in the working examples set forth below.

Compound B is prepared from Compound A by reaction with a suitable sulfonyl azide, such as 4-acetamidobenzenesulfonyl azide, in the presence of a suitable base, such as DBU, in a suitable solvent such as ACN. Compound B is converted to Compound C by reaction with a suitable amine, wherein R^(a) and R^(b) are as defined for R⁶ and R⁷, in the presence of a suitable catalyst, such as Rh₂(OAc)₄, in a suitable solvent, such as toluene. Compound C is hydrolized to Compound D by treatment with a suitable acid, such as conc. HCl. Compound D is converted to Compound F by first conversion to acid chloride E by treatment with a suitable reagent, such as oxalyl chloride, in a suitable solvent, such as DCM, followed by reaction of Compound E with an excess of a suitable amine, where R^(e) and R^(d) are as defined for R⁶ and R⁷, in a suitable solvent, such as THF.

Compound G is converted to Compound H by treatment with a suitable acid, such as TFA, in a suitable solvent, such as DCM.

Compound H is converted to Compound I by reaction with a suitable reagent, such as (bis(trifluoroacetoxy)iodo)benzene, in a suitable solvent, such as aq. ACN. Compound H can also be dehydrated to Compound J by treatment with a suitable reagent, such as TFAA, in the presence of a suitable base, such as DIPEA, in a suitable solvent, such as THF.

Compound K can be converted to Compound L by hydrogenation over a suitable catalyst, such as Pd(OH)₂, in a suitable solvent, such as AcOH. Compound L can be converted to Compound M by alkylation with a suitable alkyl halide, triflate, tosylate, mesylate, etc. in the presence of a suitable base, such as DIPEA, in a suitable solvent, such as ACN. Compound L can also be converted to Compound M by reductive ammination with the appropriate aldehyde/ketone in the presence of a suitable reducing agent, such as NaBH(OAc)₃, in a suitable solvent, such as DCM. In Scheme D, R^(e) can be, for example, an alkyl group optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups.

Subsequent side chain modifications can be accomplished via appropriate functional group manipulations known to one skilled in the art.

The starting compounds having the formula

can be prepared, for example, as described in Hupp C. D., et al., Tetrahedron Letters 51:2359-2361 (2010) and Ida Y., et al., Bioorganic & Medical Chemistry 20:949-961 (2012).

The starting compounds having the formula

can be prepared, for example, as described in Polazzi J. O. et al., J. Med. Chem. 23:174-179 (1980).

The opposite isomer of Compound A′, having the formula

can be prepared according methods described in the art, such as, for example, in US 2009/0156818, US 2009/0156820, and Hupp C. D., et al. (supra). Accordingly, for example, Compound A′″, where R¹ is OMe and R² is cyclopropylmethyl, can be prepared as described in Scheme E starting from CAS#6080-33-7:

Further, Compounds of the Invention where R¹ is CN or aminocarbonyl can be prepared, e.g., as shown in Scheme F below.

One of the hydroxyl groups in Compound B1 can be selectedly protected as triflate to yield Compound B2 through a reaction using a suitable triflate reagent (such as, N-Phenyl-bis(trifluoromethanesulfonimide)) in the presence of a suitable salt (such as, caesium carbonate) in a suitable solvent (such as, THF). The triflate group (TfO—) in Compound B2 can then be converted to a nitrile (—CN) group by reacting Compound B2 with a suitable cyanide salt (such as, zinc cyanide) in the presence of a suitable catalyst, such as, PdCl₂(dppf) with zinc dust, in a suitable solvent (such as, DMF) to offer Compound B3, which can further be converted to Compound B4 in a suitable catalyst (such as, hydrido(dimethylphosphinous acid-kP)[hydrogen bis(dimethyl-phosphinito-kP)]-platinum (II)) in a suitable solvent, such as, 1:1 EtOH/H₂O.

TESTING OF COMPOUNDS

In Vitro Assay Protocols

μ-Opioid Receptor Binding Assay Procedures: Radioligand dose-displacement binding assays for μ-opioid receptors used 0.3 nM [³H]-diprenorphine (Perkin Elmer, Shelton, Conn.), with 5 mg membrane protein/well in a final volume of 500 μl binding buffer (10 mM MgCl₂, 1 mM EDTA, 5% DMSO, 50 mM HEPES, pH 7.4). Reactions were carried out in the absence or presence of increasing concentrations of unlabeled naloxone. All reactions were conducted in 96-deep well polypropylene plates for 2 hours at room temperature. Binding reactions were terminated by rapid filtration onto 96-well Unifilter GF/C filter plates (Perkin Elmer, Shelton, Conn.), presoaked in 0.5% polyethylenimine using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by performing three filtration washes with 500 μl of ice-cold binding buffer. Filter plates were subsequently dried at 50° C. for 2-3 hours. BetaScint scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added (50 μl/well), and plates were counted using a Packard Top-Count for 1 min/well. The data were analyzed using the one-site competition curve fitting functions in GraphPad PRISM™ v. 3.0 or higher (San Diego, Calif.), or an in-house function for one-site competition curve-fitting.

μ-Opioid Receptor Binding Data: Generally, the lower the K_(i) value, the more effective Compounds of the Invention will be at treating or preventing pain or another Condition. In certain embodiments, Compounds of the Invention exhibit a K_(i) (nM) of about 10,000 or less for binding to μ-opioid receptors. Typically, Compounds of the Invention exhibit a K_(i) (nM) of about 1000 or less for binding to μ-opioid receptors. In one embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 300 or less for binding to μ-opioid receptors. In another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 100 or less for binding to μ-opioid receptors. In another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 10 or less for binding to μ-opioid receptors. In still another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 1 or less for binding to μ-opioid receptors. In still another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 0.1 or less for binding to μ-opioid receptors.

μ-Opioid Receptor Functional Assay Procedures: [³⁵S]GTPγS functional assays were conducted using freshly thawed μ-receptor membranes prepared in-house from a cell line expressing recombinant μ opioid receptor in a HEK-293, CHO or U-2 OS cell background, or purchased from a commercial source (Perkin Elmer, Shelton, Conn.; or DiscovRx, Fremont, Calif.). Assay reactions were prepared by sequentially adding the following reagents to binding buffer (100 mM NaCl, 10 mM MgCl₂, 20 mM HEPES, pH 7.4) on ice (final concentrations indicated): membrane protein (0.026 mg/mL), saponin (10 mg/mL), GDP (3 mM) and [³⁵S]GTPγS (0.20 nM; Perkin Elmer, Shelton, Conn.). The prepared membrane solution (190 μl/well) was transferred to 96-shallow well polypropylene plates containing 10 μl of 20× concentrated stock solutions of the agonist [D-Ala², N-methyl-Phe⁴ Gly-ol⁵]-enkephalin (DAMGO) prepared in dimethyl sulfoxide (DMSO). Plates were incubated for 30 min at about 25° C. with shaking Reactions were terminated by rapid filtration onto 96-well Unifilter GF/B filter plates (Perkin Elmer, Shelton, Conn.) using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by three filtration washes with 200 μl of ice-cold wash buffer (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, pH 7.4). Filter plates were subsequently dried at 50° C. for 2-3 hours. BetaScint scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added (50 μl/well) and plates were counted using a Packard Top-Count for 1 min/well. Data were analyzed using the sigmoidal dose-response curve fitting functions in GraphPad PRISM v. 3.0, or an in-house function for non-linear, sigmoidal dose-response curve-fitting. [³⁵S]GTPγS functional assays can also be conducted using freshly thawed μ-receptor membranes prepared from a cell line expressing recombinant μ opioid receptor in a CHO-K1 cell background.

μ-Opioid Receptor Functional Data: μ GTP EC₅₀ is the concentration of a compound providing 50% of the maximal response for the compound at a μ-opioid receptor. Typically, Compounds of the Invention exhibit a μ GTP EC₅₀ (nM) of about 5000 or less. In certain embodiments, Compounds of the Invention exhibit a μ GTP EC₅₀ (nM) of about 2000 or less; or about 1000 or less; or about 100 or less; or about 10 or less; or about 1 or less; or about 0.1 or less.

μ GTP E_(max) (%) is the maximal effect elicited by a compound relative to the effect elicited by DAMGO, a standard μ agonist. Generally, the μ GTP E_(max) (%) value measures the efficacy of a compound to treat or prevent pain or other Conditions. Typically, Compounds of the Invention exhibit a μ GTP E_(max) (%) of greater than about 10%; or greater than about 20%. In certain embodiments, Compounds of the Invention exhibit a μ GTP Emax (%) of greater than about 50%; or greater than about 65%; or greater than about 75%; or greater than about 85%; or greater than about 100%.

κ-Opioid Receptor Binding Assay Procedures: Membranes from HEK-293 cells, CHO cells or U-2 OS cells expressing the recombinant human kappa opioid receptor (κ) were prepared by lysing cells in ice cold hypotonic buffer (2.5 mM MgCl₂, 50 mM HEPES, pH 7.4) (10 mL/10 cm dish) followed by homogenization with a tissue grinder/Teflon pestle. Membranes from a cell line naturally expressing kappa opioid receptor can also be used. Membranes were collected by centrifugation at 30,000×g for 15 min at 4° C. and pellets were resuspended in hypotonic buffer to a final concentration of 1-3 mg/mL. Protein concentrations were determined using the BioRad protein assay reagent with bovine serum albumen as standard. Aliquots of κ receptor membranes were stored at −80° C.

Radioligand dose displacement assays used 0.4 nM [³H]-U69,593 (GE Healthcare, Piscataway, N.J.; 40 Ci/mmole) with 15 μg membrane protein (recombinant opioid receptor expressed in HEK 293 cells; in-house prep) in a final volume of 200 μl binding buffer (5% DMSO, 50 mM Trizma base, pH 7.4). Non-specific binding was determined in the presence of 10 μM unlabeled naloxone or U69,593. All reactions were performed in 96-well polypropylene plates for 1 hour at a temperature of about 25° C. Binding reactions were terminated by rapid filtration onto 96-well Unifilter GF/C filter plates (Perkin Elmer, Shelton, Conn.) presoaked in 0.5% polyethylenimine (Sigma). Harvesting was performed using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by five filtration washes with 200 μl ice-cold binding buffer. Filter plates were subsequently dried at 50° C. for 1-2 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added and plates were counted in a Packard Top-Count for 1 min/well.

κ-Opioid Receptor Binding Data: In certain embodiments, Compounds of the Invention exhibit a K_(i) (nM) for κ receptors of about 10,000 or more (which, for purposes of this invention, is interpreted as having no binding to the κ receptors). Certain Compounds of the Invention exhibit a K_(i) (nM) of about 20,000 or less for κ receptors. In certain embodiments, Compounds of the Invention exhibit a K_(i) (nM) of about 10,000 or less; or about 5000 or less; or about 1000 or less; or about 500 or less; or about 450 or less; or about 350 or less; or about 200 or less; or about 100 or less; or about 50 or less; or about 10 or less; or about 1 or less; or about 0.1 or less for κ receptors.

κ-Opioid Receptor Functional Assay Procedures: Functional [³⁵S]GTPγS binding assays were conducted as follows. κ opioid receptor membrane solution was prepared by sequentially adding final concentrations of 0.026 μg/μl κ membrane protein (in-house), 10 μg/mL saponin, 3 μM GDP and 0.20 nM [³⁵S]GTPγS to binding buffer (100 mM NaCl, 10 mM MgCl₂, 20 mM HEPES, pH 7.4) on ice. The prepared membrane solution (190 μl/well) was transferred to 96-shallow well polypropylene plates containing 10 μl of 20× concentrated stock solutions of agonist prepared in DMSO. Plates were incubated for 30 min at a temperature of about 25° C. with shaking. Reactions were terminated by rapid filtration onto 96-well Unifilter GF/B filter plates (Perkin Elmer, Shelton, Conn.) using a 96-well tissue harvester (Packard) and followed by three filtration washes with 200 μl ice-cold binding buffer (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, pH 7.4). Filter plates were subsequently dried at 50° C. for 2-3 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added and plates were counted in a Packard Top-Count for 1 min/well.

κ-Opioid Receptor Functional Data: κ GTP EC₅₀ is the concentration of a compound providing 50% of the maximal response for the compound at a κ receptor. Certain Compounds of the Invention exhibit a κ GTP EC₅₀ (nM) of about 20,000 or less to stimulate κ opioid receptor function. In certain embodiments, Compounds of the Invention exhibit a κ GTP EC₅₀ (nM) of about 10,000 or less; or about 5000 or less; or about 2000 or less; or about 1500 or less; or about 1000 or less; or about 600 or less; or about 100 or less; or about 50 or less; or about 25 or less; or about 10 or less; or about 1 or less; or about 0.1 or less.

κ GTP E_(max) (%) is the maximal effect elicited by a compound relative to the effect elicited by U69,593. Certain Compounds of the Invention exhibit a κ GTP E_(max) (%) of greater than about 1%; or greater than about 5%; or greater than about 10%; or greater than about 20%. In certain embodiments, Compounds of the Invention exhibit a κ GTP E_(max) (%) of greater than about 50%; or greater than about 75%; or greater than about 90%; or greater than about 100%.

δ-Opioid Receptor Binding Assay Procedures: δ-Opioid Receptor Binding Assay Procedures were conducted as follows. Radioligand dose-displacement assays used 0.3 nM [³H]-Naltrindole (Perkin Elmer, Shelton, Conn.; 33.0 Ci/mmole) with 5 μg membrane protein (Perkin Elmer, Shelton, Conn.) in a final volume of 500 μl binding buffer (5 mM MgCl₂, 5% DMSO, 50 mM Trizma base, pH 7.4). Non-specific binding was determined in the presence of 25 μM unlabeled naloxone. All reactions were performed in 96-deep well polypropylene plates for 1 hour at a temperature of about 25° C. Binding reactions were terminated by rapid filtration onto 96-well Unifilter GF/C filter plates (Perkin Elmer, Shelton, Conn.) presoaked in 0.5% polyethylenimine (Sigma). Harvesting was performed using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by five filtration washes with 500 μl ice-cold binding buffer. Filter plates were subsequently dried at 50° C. for 1-2 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added and plates are counted in a Packard Top-Count for 1 min/well.

δ-Opioid Receptor Binding Data: In certain embodiments, Compounds of the Invention exhibit a K_(i) (nM) for δ receptors of about 10,000 or more (which, for the purposes of this invention, is interpreted as having no binding to the δ receptors). Certain Compounds of the Invention exhibit a K_(i) (nM) of about 20,000 or less for δ receptors. In one embodiment, Compounds of the Invention exhibit a Ki (nM) of about 10,000 or less; or of about 9000 or less for δ receptors. In another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 7500 or less; or of about 6500 or less; or of about 5000 or less; or of about 3000 or less; or of about 2500 or less for δ receptors. In another embodiment, Compounds of the Invention exhibit a K_(i) (nM) of about 1000 or less; or of about 500 or less; or of about 350 or less; or of about 250 or less; or of about 100 or less; or of about 10 or less for δ receptors.

δ-Opioid Receptor Functional Assay Procedures: Functional [³⁵S]GTPγS binding assays were conducted as follows. δ opioid receptor membrane solution was prepared by sequentially adding final concentrations of 0.026 μg/μl δ membrane protein (Perkin Elmer, Shelton, Conn.), 10 μg/mL saponin, 3 μM GDP and 0.20 nM [³⁵S]GTPγS to binding buffer (100 mM NaCl, 10 mM MgCl₂, 20 mM HEPES, pH 7.4) on ice. The prepared membrane solution (190 μl/well) was transferred to 96-shallow well polypropylene plates containing 10 μl of 20× concentrated stock solutions of agonist prepared in DMSO. Plates were incubated for 30 min at a temperature of about 25° C. with shaking Reactions were terminated by rapid filtration onto 96-well Unifilter GF/B filter plates (Perkin Elmer, Shelton, Conn.) using a 96-well tissue harvester (Packard) and followed by three filtration washes with 200 μl ice-cold binding buffer (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, pH 7.4). Filter plates were subsequently dried at 50° C. for 1-2 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added and plates were counted in a Packard Top-count for 1 min/well.

δ-Opioid Receptor Functional Data: δ GTP EC₅₀ is the concentration of a compound providing 50% of the maximal response for the compound at a δ receptor. Certain Compounds of the Invention exhibit a δ GTP EC₅₀ (nM) of about 20,000 or less; or about 10,000 or less. In certain embodiments, Compounds of the Invention exhibit a δ GTP EC₅₀ (nM) of about 3500 or less; or of about 1000 or less; or of about 500 or less; or of about 100 or less; or of about 90 or less; or of about 50 or less; or of about 25 or less; or of about 10 or less.

δ GTP E_(max) (%) is the maximal effect elicited by a compound relative to the effect elicited by met-enkephalin. Certain Compounds of the Invention exhibit a δ GTP E_(max) (%) of greater than about 1%; or of greater than about 5%; or of greater than about 10%. In one embodiment, Compounds of the Invention exhibit a δ GTP E_(max) (%) of greater than about 30%. In another embodiment, Compounds of the Invention exhibit a δ GTP E_(max) (%) of greater than about 50%; or of greater than about 75%; or of greater than about 90%. In another embodiment, Compounds of the Invention exhibit a δ GTP E_(max) (%) of greater than about 100%.

ORL-1 Receptor Binding Assay Procedure: Membranes from recombinant HEK-293 cells expressing the human opioid receptor-like receptor (ORL-1) (Perkin Elmer, Shelton, Conn.) was prepared by lysing cells in ice-cold hypotonic buffer (2.5 mM MgCl₂, 50 mM HEPES, pH 7.4) (10 ml/10 cm dish) followed by homogenization with a tissue grinder/Teflon pestle. Membranes were collected by centrifugation at 30,000×g for 15 min at 4° C. and pellets resuspended in hypotonic buffer to a final concentration of 1-3 mg/ml. Protein concentrations were determined using the BioRad protein assay reagent with bovine serum albumen as standard. Aliquots of the ORL-1 receptor membranes were stored at −80° C.

Radioligand binding assays (screening and dose-displacement) use 0.1 nM [³H]-nociceptin (Perkin Elmer, Shelton, Conn.; 87.7 Ci/mmole) with 12 μg membrane protein in a final volume of 500 μl binding buffer (10 mM MgCl₂, 1 mM EDTA, 5% DMSO, 50 mM HEPES, pH 7.4). Non-specific binding was determined in the presence of 10 nM unlabeled nociceptin (American Peptide Company). All reactions were performed in 96-deep well polypropylene plates for 1 h at room temperature. Binding reactions were terminated by rapid filtration onto 96-well Unifilter GF/C filter plates (Perkin Elmer, Shelton, Conn.) presoaked in 0.5% polyethylenimine (Sigma). Harvesting was performed using a 96-well tissue harvester (Perkin Elmer, Shelton, Conn.) followed by three filtration washes with 500 μl ice-cold binding buffer. Filter plates were subsequently dried at 50° C. for 2-3 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) was added and plates are counted in a Packard Top-Count for 1 min/well. The data from screening and dose-displacement experiments were analyzed using Microsoft Excel and the curve fitting functions in GraphPad PRISM™, v. 3.0 or higher, respectively, or an in-house function for one-site competition curve-fitting.

ORL-1 Receptor Binding Data: Certain Compounds of the Invention can have a K_(i) (nM) of about 5000 or less. In one embodiment, certain Compounds of the Invention can have a K_(i) (nM) of about 1000 or less. In one embodiment, certain Compounds of the Invention can have a K_(i) (nM) of about 500 or less. In other embodiments, the Compounds of the Invention can have a K_(i) (nM) of about 300 or less; or of about 100 or less; or of about 50 or less; or of about 20 or less. In yet other embodiments, the Compounds of the Invention can have a K_(i) (nM) of about 10 or less; or of about 1 or less; or of about 0.1 or less.

ORL-1 Receptor Functional Assay Procedure: Membranes from recombinant HEK-293 cells expressing the human opioid receptor-like (ORL-1) (Perkin Elmer, Shelton, Conn.) can be prepared by lysing cells in ice-cold hypotonic buffer (2.5 mM Mg Cl₂, 50 mM HEPES, pH 7.4) (10 ml/10 cm dish) followed by homogenization with a tissue grinder/Teflon pestle. Membranes are collected by centrifugation at 30,000×g for 15 min at 4° C., and pellets resuspended in hypotonic buffer to a final concentration of 1-3 mg/ml. Protein concentrations are determined using the BioRad protein assay reagent with bovine serum albumen as standard. Aliquots of the ORL-1 receptor membranes are stored at −80° C.

Functional [³⁵S]GTPγS binding assays are conducted as follows. ORL-1 membrane solution is prepared by sequentially adding final concentrations of 0.026 μg/μl ORL-1 membrane protein, 10 μg/ml saponin, 3 μM GDP and 0.20 nM [³⁵S]GTPγS to binding buffer (100 mM NaCl, 10 mM MgCl₂, 20 mM HEPES, pH 7.4) on ice. The prepared membrane solution (190 μl/well) is transferred to 96-shallow well polypropylene plates containing 10 μl of 20× concentrated stock solutions of agonist/nociceptin prepared in DMSO. Plates are incubated for 30 min at room temperature with shaking. Reactions are terminated by rapid filtration onto 96-well Unifilter GF/B filter plates (Perkin Elmer, Shelton, Conn.) using a 96-well tissue harvester (Packard) and followed by three filtration washes with 200 μl ice-cold binding buffer (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, pH 7.4). Filter plates are subsequently dried at 50° C. for 2-3 hours. Fifty μl/well scintillation cocktail (Perkin Elmer, Shelton, Conn.) is added and plates are counted in a Packard Top-Count for 1 min/well. Data are analyzed using the sigmoidal dose-response curve fitting functions in GraphPad PRISM v. 3.0 or higher, or an in-house function for non-linear, sigmoidal dose-response curve-fitting.

ORL-1 Receptor Functional Data: ORL-1 GTP EC₅₀ is the concentration of a compound providing 50% of the maximal response for the compound at an ORL-1 receptor. In certain embodiments, the Compounds of the Invention that have a high binding affinity (i.e. low K_(i) value) can have an ORL-1 GTP EC₅₀ (nM) of greater than about 10,000 (i.e. will not stimulate at therapeutic concentrations) In certain embodiments Compounds of the Invention can have an ORL-1 GTP EC₅₀ (nM) of about 20,000 or less. In one embodiment, the Compounds of the Invention can have an ORL-1 GTP EC₅₀ (nM) of about 10,000 or less; or of about 5000 or less; or of about 1000 or less. In still other embodiments, the Compounds of the Invention can have an ORL-1 GTP EC₅₀ (nM) of about 100 or less; or of about 10 or less; or of about 1 or less; or of about 0.1 or less.

ORL-1 GTP E_(max)% is the maximal effect elicited by a compound relative to the effect elicited by nociceptin, a standard ORL-1 agonist. In certain embodiments, Compounds of the Invention can have an ORL-1 GTP E_(max) of less than 10% (which, for the purposes of this invention, is interpreted as having antagonist activity at ORL-1 receptors). Certain Compounds of the Invention can have an ORL-1 GTP E_(max) (%) of greater than 1%; or of greater than 5%; or of greater than 10%. In other embodiments, Compounds of the Invention can have an ORL-1 GTP E_(max) of greater than 20%; or of greater than 50%; or of greater than 75%; or of greater than 88%; or of greater than 100%.

In Vivo Assays for Pain

Test Animals: Each experiment uses rats weighing between 200-260 g at the start of the experiment. The rats are group-housed and have free access to food and water at all times, except prior to oral administration of a Compound of the Invention when food is removed for about 16 hours before dosing. A control group acts as a comparison to rats treated with a Compound of the Invention. The control group is administered the carrier for the Compound of the Invention. The volume of carrier administered to the control group is the same as the volume of carrier and Compound of the Invention administered to the test group.

Acute Pain: To assess the actions of a Compound of the Invention for the treatment or prevention of acute pain, the rat tail flick can be used. Rats are gently restrained by hand and the tail exposed to a focused beam of radiant heat at a point 5 cm from the tip using a tail flick unit (Model 7360, commercially available from Ugo Basile of Italy). Tail flick latencies are defined as the interval between the onset of the thermal stimulus and the flick of the tail. Animals not responding within 20 seconds are removed from the tail flick unit and assigned a withdrawal latency of 20 seconds. Tail flick latencies are measured immediately before (pre-treatment) and 1, 3, and 5 hours following administration of a Compound of the Invention. Data are expressed as tail flick latency(s) and the percentage of the maximal possible effect (% MPE), i.e., 20 seconds, is calculated as follows:

${\%\mspace{14mu} M\; P\; E} = {\frac{\left\lbrack {\left( {{post}\mspace{14mu}{administration}\mspace{14mu}{latency}} \right) - \left( {{pre}\text{-}{administration}\mspace{14mu}{latency}} \right)} \right\rbrack}{\left( {{20\mspace{14mu} s} - {{pre}\text{-}{administration}\mspace{14mu}{latency}}} \right)} \times 100}$ The rat tail flick test is described in F. E. D'Amour et al., “A Method for Determining Loss of Pain Sensation,” J. Pharmacol. Exp. Ther. 72:74-79 (1941).

To assess the actions of a Compound of the Invention for the treatment or prevention of acute pain, the rat hot plate test can also be used. Rats are tested using a hot plate apparatus consisting of a clear plexiglass cylinder with a heated metal floor maintained at a temperature of 48-52° C. (Model 7280, commercially available from Ugo Basile of Italy). A rat is placed into the cylinder on the hot plate apparatus for a maximum duration of 30 s, or until it exhibits a nocifensive behavior (behavioral endpoint), at which time it is removed from the hot plate, and the response latency recorded. Hot plate latencies are measured immediately before (pre-treatment) and 1, 3, and 5 hours following administration of a Compound of the Invention. The nocifensive behavioral endpoint is defined as any of the following: 1) paw withdrawal, either as a sustained lift or with shaking or licking; 2) alternating foot lifting; 3) excape or attempted escape from the testing device; or 4) vocalization. Data are expressed as response latency(s) and the percentage of the maximal possible effect is calculated as described above for the tail flick test. The hot plate test is described in G. Woolfe and A. D. MacDonald, J. Pharmacol. Exp. Ther. 80:300-307 (1944).

Inflammatory Pain: To assess the actions of a Compound of the Invention for the treatment or prevention of inflammatory pain, the Freund's complete adjuvant (“FCA”) model of inflammatory pain can be used. FCA-induced inflammation of the rat hind paw is associated with the development of persistent inflammatory mechanical hyperalgesia and provides reliable prediction of the anti-hyperalgesic action of clinically useful analgesic drugs (L. Bartho et al., “Involvement of Capsaicin-sensitive Neurones in Hyperalgesia and Enhanced Opioid Antinociception in Inflammation,” Naunyn-Schmiedeberg's Archives of Pharmacol. 342:666-670 (1990)). The left hind paw of each animal is administered a 50 μL intraplantar injection of 50% FCA. Prior to injection of FCA (baseline) and 24 hour post injection, the animal is assessed for response to noxious mechanical stimuli by determining the PWT, as described below. Rats are then administered a single injection of 1, 3, or 10 mg/kg of either a Compound of the Invention; 30 mg/kg of a control drug selected from Celebrex, indomethacin or naproxen; or carrier. Responses to noxious mechanical stimuli are determined 1, 3, 5 and 24 hours post administration. Percentage reversal of hyperalgesia for each animal is defined as:

${\%\mspace{14mu}{Reversal}} = {\frac{\left\lbrack {\left( {{post}\mspace{14mu}{administration}\mspace{14mu} P\; W\; T} \right) - \left( {{pre}\text{-}{administration}\mspace{14mu} P\; W\; T} \right)} \right\rbrack}{\left\lbrack {\left( {{baseline}\mspace{14mu} P\; W\; T} \right) - \left( {{pre}\text{-}{administration}\mspace{14mu} P\; W\; T} \right)} \right\rbrack} \times 100}$

Neuropathic Pain: To assess the actions of a Compound of the Invention for the treatment or prevention of neuropathic pain, either the Seltzer model or the Chung model can be used.

In the Seltzer model, the partial sciatic nerve ligation model of neuropathic pain is used to produce neuropathic hyperalgesia in rats (Z. Seltzer et al., “A Novel Behavioral Model of Neuropathic Pain Disorders Produced in Rats by Partial Sciatic Nerve Injury,” Pain 43:205-218 (1990)). Partial ligation of the left sciatic nerve is performed under isoflurane/O₂ inhalation anaesthesia. Following induction of anesthesia, the left thigh of the rat is shaved and the sciatic nerve exposed at high thigh level through a small incision and is carefully cleared of surrounding connective tissues at a site near the trocanther just distal to the point at which the posterior biceps semitendinosus nerve branches off of the common sciatic nerve. A 7-0 silk suture is inserted into the nerve with a ⅜ curved, reversed-cutting mini-needle and tightly ligated so that the dorsal ⅓ to ½ of the nerve thickness is held within the ligature. The wound is closed with a single muscle suture (4-0 nylon (Vicryl)) and vetbond tissue glue. Following surgery, the wound area is dusted with antibiotic powder. Sham-treated rats undergo an identical surgical procedure except that the sciatic nerve is not manipulated. Following surgery, animals are weighed and placed on a warm pad until they recover from anesthesia. Animals are then returned to their home cages until behavioral testing begins. The animal is assessed for response to noxious mechanical stimuli by determining PWT, as described below, prior to surgery (baseline), then immediately prior to and 1, 3, and 5 hours after drug administration. Percentage reversal of neuropathic hyperalgesia is defined as:

${\%\mspace{14mu}{Reversal}} = {\frac{\left\lbrack {\left( {{post}\mspace{14mu}{administration}\mspace{14mu} P\; W\; T} \right) - \left( {{pre}\text{-}{administration}\mspace{14mu} P\; W\; T} \right)} \right\rbrack}{\left\lbrack {\left( {{baseline}\mspace{14mu} P\; W\; T} \right) - \left( {{pre}\text{-}{administration}\mspace{14mu} P\; W\; T} \right)} \right\rbrack} \times 100}$

In the Chung model, the spinal nerve ligation model of neuropathic pain is used to produce mechanical hyperalgesia, thermal hyperalgesia and tactile allodynia in rats. Surgery is performed under isoflurane/O₂ inhalation anaesthesia. Following induction of anaesthesia, a 3 cm incision is made and the left paraspinal muscles are separated from the spinous process at the L₄-S₂ levels. The L₆ transverse process is carefully removed with a pair of small rongeurs to identify visually the L₄-L₆ spinal nerves. The left L₅ (or L₅ and L₆) spinal nerve(s) is isolated and tightly ligated with silk thread. A complete hemostasis is confirmed and the wound is sutured using non-absorbable sutures, such as nylon sutures or stainless steel staples. Sham-treated rats undergo an identical surgical procedure except that the spinal nerve(s) is not manipulated. Following surgery animals are weighed, administered a subcutaneous (s.c.) injection of saline or ringers lactate, the wound area is dusted with antibiotic powder and they are kept on a warm pad until they recover from the anesthesia. Animals are then returned to their home cages until behavioral testing begins. The animals are assessed for response to noxious mechanical stimuli by determining PWT, as described below, prior to surgery (baseline), then immediately prior to and 1, 3, and 5 hours after being administered a Compound of the Invention. The animal can also be assessed for response to noxious thermal stimuli or for tactile allodynia, as described below. The Chung model for neuropathic pain is described in S. H. Kim, “An Experimental Model for Peripheral Neuropathy Produced by Segmental Spinal Nerve Ligation in the Rat,” Pain 50(3):355-363 (1992).

Response to Mechanical Stimuli as an Assessment of Mechanical Hyperalgesia: The paw pressure assay can be used to assess mechanical hyperalgesia. For this assay, hind paw withdrawal thresholds (PWT) to a noxious mechanical stimulus are determined using an analgesymeter (Model 7200, commercially available from Ugo Basile of Italy) as described in C. Stein, “Unilateral Inflammation of the Hindpaw in Rats as a Model of Prolonged Noxious Stimulation: Alterations in Behavior and Nociceptive Thresholds,” Pharmacol. Biochem. and Behavior 31:451-455 (1988). The rat is gently restrained, its hindpaw is placed on a small round platform, and punctate pressure is applied to the dorsal surface of the hindpaw in a graded manner. The maximum weight that is applied to the hind paw is set at 250 g and the end point is taken as complete withdrawal of the paw. PWT is determined once for each rat at each time point and either only the affected (ipsilateral; same side as the injury) rear paw is tested, or both the ipsilateral and contralateral (non-injured; opposite to the injury) rear paw are tested.

Response to Thermal Stimuli as an Assessment of Thermal Hyperalgesia: The plantar test can be used to assess thermal hyperalgesia. For this test, hind paw withdrawal latencies to a noxious thermal stimulus applied to the plantar surface of the hindpaw are determined using a plantar test apparatus (commercially available from Ugo Basile of Italy) following the technique described by K. Hargreaves et al., “A New and Sensitive Method for Measuring Thermal Nociception in Cutaneous Hyperalgesia,” Pain 32(1):77-88 (1988). The maximum exposure time is set at 32 seconds to avoid tissue damage and any directed paw withdrawal from the heat source is taken as the end point. Three latencies are determined at each time point and averaged. Either only the affected (ipsilateral) paw is tested, or both the ipsilateral and contralateral (non-injured) paw are tested.

Assessment of Tactile Allodynia: To assess tactile allodynia, rats are placed in clear, plexiglass compartments with a wire mesh floor and allowed to habituate for a period of at least 15 minutes. After habituation, a series of von Frey monofilaments are presented to the plantar surface of the affected (ipsilateral) foot of each rat. The series of von Frey monofilaments consists of six monofilaments of increasing diameter, with the smallest diameter fiber presented first. Five trials are conducted with each filament with each trial separated by approximately 2 minutes. Each presentation lasts for a period of 4-8 seconds or until a nociceptive withdrawal behavior is observed. Flinching, paw withdrawal or licking of the paw are considered nociceptive behavioral responses.

Assessment of Respiratory Depression: To assess respiratory depression, rats can be prepared by implanting a femoral artery cannula via which blood samples are taken. Blood samples are taken prior to drug administration, then 1, 3, 5 and 24 hours post-treatment. Blood samples are processed using an arterial blood gas analyzer (e.g., IDEXX VetStat with Respiratory/Blood Gas test cartridges). Comparable devices are a standard tool for blood gas analysis (e.g., D. Torbati et al., Intensive Care Med. (26): 585-591 (2000).

Assessment of Gastric Motility: Animals are treated with vehicle, reference compound or test article by oral gavage at a volume of 10 mL/kg. At one hour post-dose, all animals are treated with charcoal meal solution (5% non-activated charcoal powder in a solution of 1% carboxymethylcellulose in water) at a volume of 10 mL/kg. At two hours post-dose (one hour post-charcoal), animals are sacrificed by carbon dioxide inhalation or isoflurane overdose and the transit of charcoal meal identified. The stomach and small intestine are removed carefully and each placed on a saline-soaked absorbent surface. The distance between the pylorus and the furthest progression of charcoal meal is measured and compared to the distance between the pylorus and the ileocecal junction. The charcoal meal transit is expressed as a percentage of small intestinal length traveled.

Pharmaceutical Compositions

Due to their activity, the Compounds of the Invention are advantageously useful in human and veterinary medicine. As described above, the Compounds of the Invention are useful for treating or preventing a Condition in a patient in need thereof. The Compounds of the Invention can be administered to any patient requiring modulation of the opioid receptors. The term “patient” as used herein refers to any animal that may experience the beneficial effects of a Compound of the Invention. Foremost such animals are mammals, e.g., humans and companion animals, although the invention is not intended to be so limited.

When administered to a patient, a Compound of the Invention can be administered as a component of a composition that comprises a pharmaceutically acceptable carrier or excipient. A Compound of the Invention can be administered by any appropriate route, as determined by the medical practitioner. Methods of administration may include intradermal, intramuscular, intraperitoneal, parenteral, intravenous, subcutaneous, intranasal, epidural, oral, sublingual, buccal, intracerebral, intravaginal, transdermal, transmucosal, rectal, by inhalation, or topical (particularly to the ears, nose, eyes, or skin). Delivery can be either local or systemic. In certain embodiments, administration will result in the release of a Compound of the Invention into the bloodstream.

Pharmaceutical compositions of the invention can take the form of solutions, suspensions, emulsions, tablets, pills, pellets, powders, multi-particulates, capsules, capsules containing liquids, capsules containing powders, capsules containing multi-particulates, lozenges, sustained-release formulations, suppositories, transdermal patches, transmucosal films, sub-lingual tablets or tabs, aerosols, sprays, or any other form suitable for use. In one embodiment, the composition is in the form of a tablet. In another embodiment, the composition is in the form of a capsule (see, e.g., U.S. Pat. No. 5,698,155). Other examples of suitable pharmaceutical excipients are described in Remington's Pharmaceutical Sciences 1447-1676 (Alfonso R. Gennaro ed., 19th ed. 1995), incorporated herein by reference.

Pharmaceutical compositions of the invention preferably comprise a suitable amount of a pharmaceutically acceptable excipient so as to provide the form for proper administration to the patient. Such a pharmaceutical excipient can be a diluent, suspending agent, solubilizer, binder, disintegrant, preservative, coloring agent, lubricant, and the like. The pharmaceutical excipient can be a liquid, such as water or an oil, including those of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil, and the like. The pharmaceutical excipient can be saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea, and the like. In addition, auxiliary, stabilizing, thickening, lubricating, and coloring agents can be used. In one embodiment, the pharmaceutically acceptable excipient is sterile when administered to a patient. Water is a particularly useful excipient when a Compound of the Invention is administered intravenously. Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid excipients, particularly for injectable solutions. Suitable pharmaceutical excipients also include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene glycol, water, ethanol, and the like. The invention compositions, if desired, can also contain minor amounts of wetting or emulsifying agents, or pH buffering agents. Specific examples of pharmaceutically acceptable carriers and excipients that can be used to formulate oral dosage forms are described in the Handbook of Pharmaceutical Excipients, American Pharmaceutical Association (1986).

In certain embodiments, the Compounds of the Invention are formulated for oral administration. A Compound of the Invention to be orally delivered can be in the form of tablets, capsules, gelcaps, caplets, lozenges, aqueous or oily solutions, suspensions, granules, powders, emulsions, syrups, or elixirs, for example. When a Compound of the Invention is incorporated into oral tablets, such tablets can be compressed, tablet triturates, enteric-coated, sugar-coated, film-coated, multiply compressed or multiply layered.

An orally administered Compound of the Invention can contain one or more additional agents such as, for example, sweetening agents such as fructose, aspartame or saccharin; flavoring agents such as peppermint, oil of wintergreen, or cherry; coloring agents; and preserving agents, and stabilizers, to provide stable, pharmaceutically palatable dosage forms. Techniques and compositions for making solid oral dosage forms are described in Pharmaceutical Dosage Forms: Tablets (Lieberman, Lachman and Schwartz, eds., 2nd ed.) published by Marcel Dekker, Inc. Techniques and compositions for making tablets (compressed and molded), capsules (hard and soft gelatin) and pills are also described in Remington's Pharmaceutical Sciences 1553-1593 (Arthur Osol, ed., 16^(th) ed., Mack Publishing, Easton, Pa. 1980). Liquid oral dosage forms include aqueous and nonaqueous solutions, emulsions, suspensions, and solutions and/or suspensions reconstituted from non-effervescent granules, optionally containing one or more suitable solvents, preservatives, emulsifying agents, suspending agents, diluents, sweeteners, coloring agents, flavoring agents, and the like. Techniques and compositions for making liquid oral dosage forms are described in Pharmaceutical Dosage Forms: Disperse Systems, (Lieberman, Rieger and Banker, eds.) published by Marcel Dekker, Inc.

When a Compound of the Invention is formulated for parenteral administration by injection (e.g., continuous infusion or bolus injection), the formulation can be in the form of a suspension, solution, or emulsion in an oily or aqueous vehicle, and such formulations can further comprise pharmaceutically necessary additives such as one or more stabilizing agents, suspending agents, dispersing agents, and the like. When a Compound of the Invention is to be injected parenterally, it can be, e.g., in the form of an isotonic sterile solution. A Compound of the Invention can also be in the form of a powder for reconstitution as an injectable formulation.

In certain embodiments, a Compound of the Invention is formulated into a pharmaceutical composition for intravenous administration. Typically, such compositions comprise sterile isotonic aqueous buffer. Where necessary, the compositions can also include a solubilizing agent. A Compound of the Invention for intravenous administration can optionally include a local anesthetic such as benzocaine or prilocaine to lessen pain at the site of the injection. Generally, the ingredients are supplied either separately or mixed together in unit dosage form, for example, as a dry lyophilized powder or water free concentrate in a hermetically sealed container such as an ampule or sachette indicating the quantity of active agent. Where a Compound of the Invention is to be administered by infusion, it can be dispensed, for example, with an infusion bottle containing sterile pharmaceutical grade water or saline. Where a Compound of the Invention is administered by injection, an ampule of sterile water for injection or saline can be provided so that the ingredients can be mixed prior to administration.

When a Compound of the Invention is to be administered by inhalation, it can be formulated into a dry aerosol, or an aqueous or partially aqueous solution.

In another embodiment, a Compound of the Invention can be delivered in a vesicle, in particular a liposome (see Langer, Science 249:1527-1533 (1990); and Treat et al., Liposomes in the Therapy of Infectious Disease and Cancer 317-327 and 353-365 (1989)).

In certain embodiments, a Compound of the Invention is administered locally. This can be achieved, for example, by local infusion during surgery, topical application, e.g., in conjunction with a wound dressing after surgery, by injection, by means of a catheter, by means of a suppository or enema, or by means of an implant, said implant being of a porous, non-porous, or gelatinous material, including membranes, such as sialastic membranes, or fibers.

In certain embodiments, a Compound of the Invention can be delivered in an immediate release form. In other embodiments, a Compound of the Invention can be delivered in a controlled-release system or sustained-release system. Controlled- or sustained-release pharmaceutical compositions can have a common goal of improving drug therapy over the results achieved by their non-controlled or non-sustained-release counterparts. In one embodiment, a controlled- or sustained-release composition comprises a minimal amount of a Compound of the Invention to treat or prevent the Condition (or a symptom thereof) in a minimum amount of time. Advantages of controlled- or sustained-release compositions include extended activity of the drug, reduced dosage frequency, and increased compliance. In addition, controlled- or sustained-release compositions can favorably affect the time of onset of action or other characteristics, such as blood levels of the Compound of the Invention, and can thus reduce the occurrence of adverse side effects.

Controlled- or sustained-release compositions can initially immediately release an amount of a Compound of the Invention that promptly produces the desired therapeutic or prophylactic effect, and gradually and continually release other amounts of the Compound of the Invention to maintain a level of therapeutic or prophylactic effect over an extended period of time. To maintain a constant level of the Compound of the Invention in the body, the Compound of the Invention can be released from the dosage form at a rate that will replace the amount of Compound of the Invention being metabolized and excreted from the body. Controlled- or sustained-release of an active ingredient can be stimulated by various conditions, including but not limited to, changes in pH, changes in temperature, concentration or availability of enzymes, concentration or availability of water, or other physiological conditions or compounds.

Controlled-release and sustained-release means for use according to the present invention may be selected from those known in the art. Examples include, but are not limited to, those described in U.S. Pat. Nos. 3,845,770; 3,916,899; 3,536,809; 3,598,123; 4,008,719; 5,674,533; 5,059,595; 5,591,767; 5,120,548; 5,073,543; 5,639,476; 5,354,556; and 5,733,566, each of which is incorporated herein by reference. Such dosage forms can be used to provide controlled- or sustained-release of one or more active ingredients using, for example, hydroxypropylmethyl cellulose, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, multiparticulates, liposomes, microspheres, or a combination thereof to provide the desired release profile in varying proportions. Suitable controlled- or sustained-release formulations known in the art, including those described herein, can be readily selected for use with the active ingredients of the invention in view of this disclosure. See also Goodson, “Dental Applications” (pp. 115-138) in Medical Applications of Controlled Release, Vol. 2, Applications and Evaluation, R. S. Langer and D. L. Wise eds., CRC Press (1984). Other controlled- or sustained-release systems that are discussed in the review by Langer, Science 249:1527-1533 (1990) can be selected for use according to the present invention. In one embodiment, a pump can be used (Langer, Science 249:1527-1533 (1990); Sefton, CRC Crit. Ref. Biomed. Eng. 14:201 (1987); Buchwald et al., Surgery 88:507 (1980); and Saudek et al., N. Engl. J. Med. 321:574 (1989)). In another embodiment, polymeric materials can be used (see Medical Applications of Controlled Release (Langer and Wise eds., 1974); Controlled Drug Bioavailability, Drug Product Design and Performance (Smolen and Ball eds., 1984); Ranger and Peppas, J. Macromol. Sci. Rev. Macromol. Chem. 23:61 (1983); Levy et al., Science 228:190 (1985); During et al., Ann. Neurol. 25:351 (1989); and Howard et al., J. Neurosurg. 71:105 (1989)). In yet another embodiment, a controlled- or sustained-release system can be placed in proximity of a target of a Compound of the Invention, e.g., the spinal column, brain, or gastrointestinal tract, thus requiring only a fraction of the systemic dose.

When in tablet or pill form, a pharmaceutical composition of the invention can be coated to delay disintegration and absorption in the gastrointestinal tract, thereby providing a sustained action over an extended period of time. Selectively permeable membranes surrounding an osmotically active driving compound are also suitable for orally administered compositions. In these latter platforms, fluid from the environment surrounding the capsule is imbibed by the driving compound, which swells to displace the agent or agent composition through an aperture. These delivery platforms can provide an essentially zero order delivery profile as opposed to the spiked profiles of immediate release formulations. A time-delay material such as glycerol monostearate or glycerol stearate can also be used. Oral compositions can include standard excipients such as mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, and magnesium carbonate. In one embodiment, the excipients are of pharmaceutical grade.

Pharmaceutical compositions of the invention include single unit dosage forms suitable for oral administration such as, but not limited to, tablets, capsules, gelcaps, and caplets that are adapted for controlled- or sustained-release.

The amount of the Compound of the Invention that is effective for the treatment or prevention of a condition can be determined by standard clinical techniques. In addition, in vitro and/or in vivo assays can optionally be employed to help identify optimal dosage ranges. The precise dose to be employed will also depend on, e.g., the route of administration and the extent of the Condition to be treated, and can be decided according to the judgment of a practitioner and/or each patient's circumstances. Variations in dosing may occur depending upon typical factors such as the weight, age, gender and physical condition (e.g., hepatic and renal function) of the patient being treated, the affliction to be treated, the severity of the symptoms, the frequency of the dosage interval, the presence of any deleterious side-effects, and the particular compound utilized, among other things.

Suitable effective dosage amounts can range from about 0.01 mg/kg of body weight to about 3000 mg/kg of body weight of the patient per day, although they are typically from about 0.01 mg/kg of body weight to about 2500 mg/kg of body weight of the patient per day or from about 0.01 mg/kg of body weight to about 1000 mg/kg of body weight of the patient per day. In one embodiment, the effective dosage amount is about 100 mg/kg of body weight of the patient per day or less. In another embodiment, the effective dosage amount ranges from about 0.01 mg/kg of body weight to about 100 mg/kg of body weight of the patient per day of a Compound of the Invention, in another embodiment, about 0.02 mg/kg of body weight to about 50 mg/kg of body weight of the patient per day, and in another embodiment, about 0.025 mg/kg of body weight to about 20 mg/kg of body weight of the patient per day.

Administration can be as a single dose or as a divided dose. In one embodiment, an effective dosage amount is administered about every 24 hours until the Condition is abated. In another embodiment, an effective dosage amount is administered about every 12 hours until the Condition is abated. In another embodiment, an effective dosage amount is administered about every 8 hours until the Condition is abated. In another embodiment, an effective dosage amount is administered about every 6 hours until the Condition is abated. In another embodiment, an effective dosage amount is administered about every 4 hours until the Condition is abated. The effective dosage amounts described herein refer to total amounts administered; that is, if more than one Compound of the Invention is administered, the effective dosage amounts correspond to the total amount administered.

Where a cell capable of expressing the t-opioid receptors is contacted with a Compound of the Invention in vitro, the amount effective for inhibiting or activating the μ-opioid receptors function in a cell can typically range from about 10⁻¹² mol/L to about 10⁻⁴ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁵ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁶ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁹ mol/L of a solution or suspension of the Compound of the Invention in a pharmaceutically acceptable carrier or excipient. In one embodiment, the volume of solution or suspension comprising the Compound of the Invention can be from about 0.01 μL to about 1 mL. In another embodiment, the volume of solution or suspension can be about 200 μL.

Where a cell capable of expressing the δ-opioid receptors is contacted with a Compound of the Invention in vitro, the amount effective for inhibiting or activating the δ-opioid receptors function in a cell can typically range from about 10⁻¹² mol/L to about 10⁻⁴ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁵ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁶ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁹ mol/L of a solution or suspension of the Compound of the Invention in a pharmaceutically acceptable carrier or excipient. In one embodiment, the volume of solution or suspension comprising the Compound of the Invention can be from about 0.01 μL to about 1 mL. In another embodiment, the volume of solution or suspension can be about 200 μL.

Where a cell capable of expressing the κ-opioid receptors is contacted with a Compound of the Invention in vitro, the amount effective for inhibiting or activating the κ-opioid receptors function in a cell can typically range from about 10⁻¹² mol/L to about 10⁴ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁵ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁶ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁹ mol/L of a solution or suspension of the Compound of the Invention in a pharmaceutically acceptable carrier or excipient. In one embodiment, the volume of solution or suspension comprising the Compound of the Invention can be from about 0.01 μL to about 1 mL. In another embodiment, the volume of solution or suspension can be about 200 μL.

Where a cell capable of expressing the ORL-1 receptor is contacted with a Compound of the Invention in vitro, the amount effective for inhibiting or activating the ORL-1 receptor function in a cell can typically range from about 10⁻¹² mol/L to about 10⁴ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁵ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁶ mol/L, or from about 10⁻¹² mol/L to about 10⁻⁹ mol/L of a solution or suspension of the compound in a pharmaceutically acceptable carrier or excipient. In one embodiment, the volume of solution or suspension comprising the Compound of the Invention can be from about 0.01 μL to about 1 mL. In another embodiment, the volume of solution or suspension can be about 200 μL.

Compounds of the Invention can be assayed in vitro or in vivo for the desired therapeutic or prophylactic activity prior to use in humans. Animal model systems can be used to demonstrate safety and efficacy. Certain Compounds of the Invention are expected to have an ED₅₀ for treating inflammatory pain ranging from about 0.5 mg/kg to about 20 mg/kg. Certain Compounds of the Invention are expected to produce significant analgesia and/or anti-hyperalgesia at doses that do not induce respiratory depression. In contrast, oxygen tension, oxygen saturation and pH are significantly decreased, while carbon dioxide is significantly increased, in blood samples from rats given effective doses of conventional opioids, such as morphine.

According to the present invention, methods for treating or preventing a Condition in apatient in need thereof can further comprise co-administering to the patient an effective amount of a second therapeutic agent in addition to a Compound of the Invention (i.e., a first therapeutic agent). An effective amount of the second therapeutic agent can be known or determinable by a medical practitioner in view of this disclosure and published clinical studies. In one embodiment of the invention, where a second therapeutic agent is administered to a patient for treatment of a Condition (e.g., pain), the minimal effective amount of the Compound of the Invention (i.e., the first therapeutic agent) will be less than its minimal effective amount would be in circumstances where the second therapeutic agent is not administered. In this embodiment, the Compound of the Invention and the second therapeutic agent can act either additively or synergistically to treat or prevent a Condition. Alternatively, the second therapeutic agent may be used to treat or prevent a disorder that is different from the Condition for which the first therapeutic agent is being administered, and which disorder may or may not be a Condition as defined hereinabove. In one embodiment, a Compound of the Invention is administered concurrently with a second therapeutic agent as a single composition comprising an effective amount of a Compound of the Invention and an effective amount of the second therapeutic agent. Alternatively, a composition comprising an effective amount of a Compound of the Invention and a second composition comprising an effective amount of the second therapeutic agent are concurrently administered. In another embodiment, an effective amount of a Compound of the Invention is administered prior or subsequent to administration of an effective amount of the second therapeutic agent. In this embodiment, the Compound of the Invention is administered while the second therapeutic agent exerts its therapeutic effect, or the second therapeutic agent is administered while the Compound of the Invention exerts its therapeutic effect for treating or preventing a Condition.

The second therapeutic agent can be, but is not limited to, an opioid agonist, a non-opioid analgesic, a non-steroidal anti-inflammatory agent, an antimigraine agent, a Cox-IA inhibitor, a 5-lipoxygenase inhibitor, an anti-emetic, a β-adrenergic blocker, an anticonvulsant, an antidepressant, a Ca²⁺-channel blocker, an anti-cancer agent, an agent for treating or preventing UI, an agent for treating or preventing anxiety, an agent for treating or preventing a memory disorder, an agent for treating or preventing obesity, an agent for treating or preventing constipation, an agent for treating or preventing cough, an agent for treating or preventing diarrhea, an agent for treating or preventing high blood pressure, an agent for treating or preventing epilepsy, an agent for treating or preventing anorexia/cachexia, an agent for treating or preventing drug abuse, an agent for treating or preventing an ulcer, an agent for treating or preventing IBD, an agent for treating or preventing IBS, an agent for treating or preventing addictive disorder, an agent for treating or preventing Parkinson's disease and parkinsonism, an agent for treating or preventing a stroke, an agent for treating or preventing a seizure, an agent for treating or preventing a pruritic condition, an agent for treating or preventing psychosis, an agent for treating or preventing Huntington's chorea, an agent for treating or preventing ALS, an agent for treating or preventing a cognitive disorder, an agent for treating or preventing a migraine, an agent for treating, preventing or inhibiting vomiting, an agent for treating or preventing dyskinesia, an agent for treating or preventing depression, or any mixture thereof.

A composition of the invention is prepared by a method comprising admixing a Compound of the Invention with a pharmaceutically acceptable carrier or excipient. Admixing can be accomplished using methods known for admixing a compound (or derivative) and a pharmaceutically acceptable carrier or excipient. In one embodiment, the Compound of the Invention is present in the composition in an effective amount.

The present invention also relates to a kit, comprising a sterile container containing an effective amount of a Compound of the Invention and instructions for therapeutic use.

The following examples are illustrative, but not limiting, of the compounds, compositions and methods of the present invention. Suitable modifications and adaptations of the variety of conditions and parameters normally encountered in clinical therapy and which are obvious to those skilled in the art in view of this disclosure are within the spirit and scope of the invention.

EXAMPLES

The following abbreviations are used:

ACN acetonitrile

AcOH acetic acid

aq. aqueous

atm atmosphere(s)

Bn benzyl

° C. degrees Celcius

conc. concentrated

DBU 1,8-diazabicyclo[5.4.0]undec-7-ene

DCE dichloroethane

DCM dichloromethane

DIPEA diisopropylethylamine

DME 1,2-dimethoxyethane

DMF dimethylformamide

DMSO dimethylsulfoxide

Et₂O diethyl ether

EtOAc ethyl acetate

EtOH ethanol

h hour(s)

HPLC high pressure liquid chromatography

i-PrOH iso-propanol

MeOH methanol

min minute(s)

MPLC medium pressure liquid chromatography

psi pounds per square inch

RT room temperature

satd. saturated

Tf trifluoromethanesulfonyl

TFA trifluoroacetic acid

TFAA trifluoroacetic anhydride

THF tetrahydrofuran

Example 1

DIPEA (13.3 mL, 76 mmol) was added to Compound 1 (27.4 g, 72.5 mmol) in Ac₂O (68.4 mL, 725 mmol) and the solution was heated at 120° C. for 2 h. The reaction mixture was diluted with EtOAc, washed with two portions of satd. aq. NaHCO₃, dried over Na₂SO₄, and concentrated. ACN (300 mL) and 4-acetamidobenzenesulfonyl azide (34.8 g, 145 mmol) were added, followed by DBU (32.8 mL, 218 mmol) at 0° C. The reaction was allowed to warm to RT over 18 h and concentrated. EtOAc was added, washed with two portions of 1M aq. NaOH, dried over Na₂SO₄ and concentrated. The resulting brown solid was triturated with acetone, filtered, and carried on without further purification to yield 17.28 g of Compound 2 as a yellow solid. LC/MS, m/z=410 [M+H]⁺ (Calc: 409).

The starting Compound 1 can be prepared as described in, for example, Hupp C. D., et al., Tetrahedron Letters 51:2359-2361 (2010).

Example 2 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2-guanidinoethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (6) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(4-guanidinobutyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (7)

(a) A solution of Compound 2 (0.5 g, 1.2 mmol) in 3 mL DCE was added slowly to tert-butyl (2-aminoethyl)carbamate (0.21 mL, 1.3 mmol) and Rh₂(OAc)₄ (27 mg, 0.6 mmol) in 2 mL toluene at 80° C. The solution was heated at reflux for 21 h, adding 2 mL toluene after 4 h, and concentrated. The resulting material was purified by MPLC (0-8% MeOH/DCM) to yield Compound 3 (mixture of diastereomers) as a brown oil.

(b) DCM (10 mL) was added to Compound 3. A 1M solution of BBr₃ in DCM (6.1 mL, 6.1 mmol) was added at 0° C. and the solution stirred at 0° C. for 30 min. The reaction was quenched with MeOH followed by 7M NH₃/MeOH and concentrated. A 25% MeOH/DCM solution was added to the resulting material, the solid removed by filtration, and the filtrate purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield 350 mg of Compound 4 (mixture of diastereomers) as a brown oil. MeOH (5 mL) was added followed by 5 drops of AcOH and the resulting solution heated at reflux for 16 h. Concentration led to Compound 5 (mixture of diastereomers) which was carried on without further purification.

(c) DIPEA (0.419 mL, 2.4 mmol) was added to a solution of Compound 5 (308 mg, 0.8 mmol) and 1H-pyrazole-1-carboxamidine hydrochloride (141 mg, 0.96 mmol) in 4 mL DMF. The reaction mixture was stirred at 80° C. for 16 h, concentrated, and triturated with DCM. The resulting solid was purified by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield Compound 6 as its TFA salt.

Compound 6 TFA salt: ¹H NMR (MeOH-d₄) δ: 7.91 (br. s., 1H), 6.93 (d, J=8.4 Hz, 1H), 6.66 (d, J=2.4 Hz, 1H), 6.56 (dd, J=8.1, 2.4 Hz, 1H), 4.12 (d, J=4.6 Hz, 1H), 2.89-3.30 (m, 9H), 2.80 (dd, J=13.5, 7.6 Hz, 1H), 2.58 (dd, J=13.2, 2.2 Hz, 1H), 2.31-2.48 (m, 3H), 2.14 (dd, J=13.4, 8.4 Hz, 1H), 1.86 (dd, J=13.5, 8.7 Hz, 1H), 1.39-1.54 (m, 1H), 0.95-1.08 (m, 1H), 0.68-0.79 (m, 1H), 0.58-0.68 (m, 1H), 0.34-0.46 (m, 2H); LC/MS, m/z=428 [M+H]⁺ (Calc: 427).

(d) Compound 7 TFA salt was prepared in an analogous fashion from Compound 2 and tert-butyl (4-aminobutyl)carbamate:

Compound 7 TFA salt: ¹H NMR (MeOH-d₄) δ: 7.62-7.69 (m, 1H), 6.93 (d, J=8.4 Hz, 1H), 6.66 (d, J=2.4 Hz, 1H), 6.56 (dd, J=8.4, 2.4 Hz, 1H), 4.11 (d, J=4.6 Hz, 1H), 3.19-3.31 (m, 3H), 2.88-3.17 (m, 6H), 2.79 (dd, J=13.4, 7.7 Hz, 1H), 2.56 (dd, J=13.1, 2.5 Hz, 1H), 2.31-2.47 (m, 3H), 2.10 (dd, J=13.4, 8.6 Hz, 1H), 1.86 (dd, J=13.5, 8.7 Hz, 1H), 1.47 (d, J=8.8 Hz, 1H), 1.28-1.42 (m, 4H), 0.95-1.08 (m, 1H), 0.69-0.78 (m, 1H), 0.60-0.68 (m, 1H), 0.35-0.46 (m, 2H).

Example 3 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (11)

Rh₂(OAc)₄ (62 mg, 0.14 mmol) was added to a solution of Compound 2 (5.79 g, 14.1 mmol) and 2,4-dimethoxybenzylamine (2.84 g, 17.0 mmol) in 75 mL toluene. The solution was heated at reflux for 17 h and concentrated to yield Compound 8 as a mixture of diastereomers. MeOH (34 mL) was added followed by 2.5M aq. NaOH (17 mL, 42.4 mmol). The solution was heated at 80° C. for 3 h. MeOH was removed under vacuum, satd. aq. NaHCO₃ was added and the aqueous layer washed with two portions of DCM. The combined organic layers were dried over Na₂SO₄ and concentrated to yield Compound 9 (mixture of diastereomers) as a brown foam. DCM (25 mL) was added followed by TFA (25 mL) and the solution stirred at RT for 2 h. An additional 25 mL aliquot of TFA was added and the solution stirred at RT for 17 h. An additional 30 mL aliquot of 1:1 DCM:TFA was added and the solution stirred at RT for 8 h and concentrated. DCM was added, washed with two portions of 10% aq. NH₄OH, one portion of brine, dried over Na₂SO₄, and concentrated. The resulting material was purified by MPLC (0-20% MeOH/DCM) to yield 1.57 g of Compound 10 and 2.54 g of Compound 11. Compound 11 was purified by preparatory HPLC [0-60% ACN/H₂O (0.01% TFA)] and isolated as its TFA salt.

Compound 11 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.73 (br. s, 1H), 7.06 (d, J=8.6 Hz, 2H), 6.83 (d, J=2.4 Hz, 1H), 6.77 (dd, J=8.4, 2.6 Hz, 1H), 6.61 (br. s., 1H), 5.85 (s, 1H), 4.07 (d, J=4.6 Hz, 1H), 3.24-3.38 (m, 2H), 3.10-3.23 (m, 1H), 2.93-3.08 (m, 2H), 2.77-2.86 (m, 1H), 2.63 (dd, J=13.1, 2.5 Hz, 1H), 2.15-2.36 (m, 3H), 1.84-1.97 (m, 2H), 1.49 (d, J=11.4 Hz, 1H), 1.02-1.14 (m, 1H), 0.63-0.73 (m, 1H), 0.55-0.63 (m, 1H), 0.45-0.53 (m, 1H), 0.37-0.45 (m, 1H); LC/MS, m/z=357 [M+H]⁺ (Calc: 356).

Example 4 (2R,3 aS,4R,9bS)-methyl 12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylate (12)

Conc. HCl (4 mL, 48 mmol) was added to Compound 11 (1.33 g, 3.88 mmol) in 4 mL water and the resulting suspension heated at 80° C. for 18 h. MeOH was added to break up the resulting sludge and the material was concentrated to yield 1.28 g of a brown solid that was carried on without further purification. To a solution of this solid (1.17 g, 3.4 mmol) in 18 mL MeOH was added SOCl₂ (0.81 g, 6.8 mmol) at 0° C. The solution was heated at reflux for 2 h and concentrated. DCM was added and the solution washed with 10% aq. NH₄OH. The resulting sludge was decanted off, the organic layer dried over Na₂SO₄, and concentrated to yield crude Compound 12 as a brown oil. Compound 12 was purified by preparatory HPLC [0-60% ACN/H2O (0.01% TFA)] and isolated as its TFA salt.

TFA salt of Compound 12: ¹H NMR (DMSO-d₆) δ: 9.28 (br. s., 1H), 8.74 (br. s., 1H), 6.93 (d, J=8.4 Hz, 1H), 6.68 (d, J=2.4 Hz, 1H), 6.60 (dd, J=8.3, 2.3 Hz, 1H), 5.96 (br. s., 1H), 4.07 (d, J=4.8 Hz, 1H), 3.40 (s, 3H), 3.23-3.37 (m, 2H), 3.09-3.23 (m, 2H), 2.92-3.03 (m, 1H), 2.76-2.87 (m, 1H), 2.66 (d, J=13.2 Hz, 1H), 2.19-2.36 (m, 3H), 1.98-2.10 (m, 1H), 1.87-1.98 (m, 1H), 1.39-1.53 (m, 1H), 0.98-1.12 (m, 1H), 0.52-0.73 (m, 2H), 0.34-0.52 (m, 2H); LC/MS, m/z=358 [M+H]⁺ (Calc: 357).

Example 5 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylic acid (14) (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylic acid (15)

Conc. HCl (3.7 mL, 44 mmol) was added to a diastereomeric mixture of Compound 10 and Compound 11 (1.57 g, 4.4 mmol) in 8 mL of 1:1 MeOH:water and the resulting suspension heated at 80° C. for 21.5 h. The reaction mixture was concentrated and purified by MPLC (0-15% (10% NH₄OH/MeOH)/DCM) to yield 1.11 g of Compound 13 as a 2:1 α:β diastereomeric mixture and 218 mg of a 2:1 α:β diastereomeric mixture of Compound 14 and Compound 15 which were separated by prep-HPLC to yield each as its TFA salt.

Compound 13: LC/MS, m/z=472 [M+H]⁺ (Calc: 471).

Compound 14 TFA salt: ¹H NMR (DMSO-d₆) δ: 11.95 (br. s., 1 H), 8.75 (br. s., 1 H), 7.07 (d, J=8.4 Hz, 1 H), 6.89 (d, J=2.2 Hz, 1 H), 6.78 (dd, J=8.5, 2.3 Hz, 1 H), 5.91 (s, 1 H), 4.08 (d, J=4.6 Hz, 1 H), 3.91 (s, 1 H), 3.71 (s, 3 H), 3.27-3.36 (m, 2 H), 3.15-3.27 (m, 2 H), 3.09 (q, J=9.2 Hz, 1 H), 2.98 (d, J=8.4 Hz, 1 H), 2.74-2.92 (m, 2 H), 2.19-2.35 (m, 3 H), 1.87-2.09 (m, 2 H), 1.52 (d, J=9.5 Hz, 1 H), 1.03-1.12 (m, 1 H), 0.65-0.73 (m, 1 H), 0.55-0.64 (m, 1 H), 0.45-0.52 (m, 1 H), 0.37-0.45 (m, 1 H). LC/MS, m/z=458 [M+H]⁺ (Calc: 457).

Compound 15 TFA salt: ¹H NMR (DMSO-d₆) δ: 12.07 (br. s., 1 H), 8.69 (br. s., 1 H), 7.03 (d, J=8.6 Hz, 1 H), 6.90 (d, J=2.4 Hz, 1 H), 6.77 (dd, J=8.6, 2.4 Hz, 1 H), 5.74 (s, 1 H), 4.01 (d, J=4.6 Hz, 2 H), 3.68 (s, 3 H), 3.11-3.31 (m, 4 H), 2.90 (d, J=10.3 Hz, 1 H), 2.72-2.82 (m, 1 H), 2.57 (dd, J=12.0, 8.3 Hz, 1 H), 2.04-2.32 (m, 4 H), 1.75-1.85 (m, 1 H), 1.44 (d, J=11.4 Hz, 1 H), 0.91-1.04 (m, 1 H), 0.57-0.66 (m, 1 H), 0.48-0.56 (m, 1 H), 0.29-0.44 (m, 2H). LC/MS, m/z=358 [M+H]⁺ (Calc: 357).

Example 6 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylic acid (16)

A 10% aq. solution of NaOH (0.9 mL, 2.3 mmol) was added to Compound 12 (270 mg, 0.76 mmol) in 4 mL MeOH and the resulting solution was stirred at RT for 90 min and at 80° C. for 6 h. The reaction was quenched with excess TFA, concentrated, and purified by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield the title Compound 16 as its TFA salt.

Compound 16 TFA salt: ¹H NMR (DMSO-d₆) δ: 11.90 (br. s., 1H), 9.19 (s, 1H), 8.65 (br. s., 1H), 6.88 (d, J=8.4 Hz, 1H), 6.64 (d, J=2.2 Hz, 1H), 6.52 (dd, J=8.4, 2.2 Hz, 1H), 5.81 (s, 1H), 3.99 (d, J=4.6 Hz, 1H), 3.17-3.28 (m, 3H), 2.99-3.13 (m, 2H), 2.90 (d, J=8.8 Hz, 1H), 2.70-2.79 (m, 1H), 2.59 (d, J=10.8 Hz, 1H), 2.12-2.28 (m, 3H), 1.88-1.97 (m, 1H), 1.79-1.88 (m, 1H), 1.38 (d, J=9.7 Hz, 1H), 0.94-1.05 (m, 1H), 0.56-0.65 (m, 1H), 0.47-0.56 (m, 1H), 0.40 (dt, J=9.4, 4.6 Hz, 1H), 0.29-0.37 (m, 1H); LC/MS, m/z=344 [M+H]⁺ (Calc: 343).

Example 7 ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-yl)(pyrrolidin-1-yl)methanone (19) ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-yl)(pyrrolidin-1-yl)methanone (20)

(a) Rh₂(OAc)₄ (88 mg, 0.2 mmol) was added to a solution of Compound 2 (4.09, 10 mmol) and pyrrolidine (0.85 g, 12.0 mmol) in 50 mL toluene. The solution was heated at reflux for 16 h, concentrated, and purified by MPLC (0-30% acetone/hexanes) to yield 1.56 g of Compound 17 and 0.86 g of Compound 18, both as brown foams.

Compound 17: LC/MS, m/z=453 [M+H]⁺ (Calc: 452).

Compound 18: LC/MS, m/z=453 [M+H]⁺ (Calc: 452).

(b) AcOH (4 drops) was added to Compound 17 (100 mg) in 1 mL MeOH and the solution heated at 60° C. for 26 h. The solution was concentrated and purified by preparatory HPLC [0-60% ACN/H₂O (0.01% TFA)] to yield the title Compound 19 as its TFA salt.

Compound 19 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.75 (br. s., 1H), 7.06 (d, J=8.6 Hz, 1 H), 6.83 (d, J=2.6 Hz, 1 H), 6.77 (dd, J=8.5, 2.5 Hz, 1 H), 5.85 (s, 1 H), 4.08 (d, J=4.0 Hz, 1 H), 3.70 (s, 3 H), 3.19-3.41 (m, 5 H), 3.07-3.19 (m, 3 H), 2.98 (d, J=12.1 Hz, 1 H), 2.77-2.87 (m, 1 H), 2.59 (dd, J=12.7, 3.2 Hz, 1 H), 2.16-2.38 (m, 3 H), 1.91-1.98 (m, 2 H), 1.81-1.91 (m, 2 H), 1.67-1.80 (m, 2 H), 1.49 (d, J=12.1 Hz, 1 H), 1.04-1.12 (m, 1 H), 0.64-0.74 (m, 1 H), 0.54-0.64 (m, 1 H), 0.46-0.53 (m, 1 H), 0.37-0.46 (m, 1 H); LC/MS, m/z=411 [M+H]⁺ (Calc: 410).

(c) Compound 20 was prepared as its TFA salt in an analogous fashion from Compound 18.

Compound 20 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.71 (br. s., 1H), 7.04 (d, J=8.6 Hz, 1 H), 6.90 (d, J=2.4 Hz, 1 H), 6.87 (br. s., 1 H), 6.77 (dd, J=8.5, 2.5 Hz, 1 H), 4.07 (d, J=5.1 Hz, 1 H), 3.69 (s, 3 H), 3.31-3.46 (m, 3 H), 3.21-3.31 (m, 4 H), 3.08-3.16 (m, 1 H), 2.84-2.97 (m, 2 H), 2.73-2.84 (m, 2 H), 2.14-2.35 (m, 2 H), 1.62-1.85 (m, 7 H), 1.46 (d, J=12.3 Hz, 1 H), 0.94-1.07 (m, 1 H), 0.58-0.67 (m, 1 H), 0.53 (s, 1 H), 0.29-0.44 (m, 2 H); LC/MS, m/z=411 [M+H]⁺ (Calc: 410).

Example 8 (2R,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a-ol (21) (2R,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a,8-diol (22)

(a) A solution of Compound 11 (0.61 g, 1.7 mmol) in 4 mL ACN was added to (bis(trifluoroacetoxy)iodo)benzene (736 mg, 1.7 mmol) in 4 mL water and the resulting mixture stirred at RT for 64.5 h. Another aliquot of (bis(trifluoroacetoxy)iodo)benzene (736 mg, 1.7 mmol) was added and the mixture stirred for an additional 26.5 h. A 10% aq. solution of HCl was added and washed with Et₂O. The aqueous layer was basified with 10% aq. NH₄OH to pH 10-11, and extracted with DCM followed by EtOAc. The DCM and EtOAc layers were combined, dried over Na₂SO₄, and concentrated. The resulting brown oil was purified by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield Compound 21 as its bis-TFA salt.

Compound 21 bis TFA salt: ¹H NMR (DMSO-d₆) δ: 8.93 (br. s., 1H), 7.72-7.86 (m, 3 H), 7.19 (d, J=8.4 Hz, 1 H), 6.88-7.00 (m, 2 H), 6.26 (br. s., 1 H), 4.17 (d, J=4.4 Hz, 1 H), 3.94 (d, J=5.7 Hz, 2 H), 3.82 (s, 3 H), 3.44 (d, J=19.6 Hz, 1 H), 3.30-3.41 (m, 1 H), 3.22 (dd, J=20.5, 5.7 Hz, 1 H), 3.08 (d, J=11.7 Hz, 1 H), 2.86-2.95 (m, 1 H), 2.46-2.53 (m, 2 H), 2.32-2.43 (m, 2 H), 2.21-2.32 (m, 1 H), 1.72 (dd, J=13.1, 8.5 Hz, 1 H), 1.60 (d, J=12.1 Hz, 1 H), 1.07-1.18 (m, 1 H), 0.69-0.79 (m, 1 H), 0.60-0.69 (m, 1 H), 0.42-0.59 (m, 2 H); LC/MS, m/z=329 [M+H]⁺ (Calc: 328).

(b) A 1M solution of BBr₃ (1.3 mL, 1.3 mmol) was added to Compound 21 (105 mg, 0.32 mmol) in 2 mL DCM and the resulting suspension stirred at RT for 75 min. The reaction was slowly quenched with MeOH, concentrated, and purified by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield Compound 22 as its bis-TFA salt.

Compound 22 bis TFA salt: ¹H NMR (DMSO-d₆) δ: 9.42 (s, 1 H), 8.81 (br. s., 1 H), 7.61-7.77 (m, 3 H), 7.01 (d, J=8.1 Hz, 1 H), 6.61-6.75 (m, 2 H), 6.10 (s, 1 H), 4.06 (d, J=3.7 Hz, 1 H), 3.80-3.92 (m, 1 H), 3.32 (d, J=19.8 Hz, 2 H), 3.11 (dd, J=19.4, 5.3 Hz, 1 H), 3.00 (d, J=10.8 Hz, 1 H), 2.77-2.89 (m, 1 H), 2.39-2.47 (m, 1 H), 2.17-2.37 (m, 4 H), 1.66 (dd, J=13.0, 8.6 Hz, 1 H), 1.47 (d, J=11.4 Hz, 1 H), 1.00-1.11 (m, 1 H), 0.63-0.72 (m, 1 H), 0.53-0.63 (m, 1 H), 0.37-0.53 (m, 2 H); LC/MS, m/z=315 [M+H]⁺ (Calc: 314).

Example 9 Preparation of Compound 23 and Compound 24

DCM (5 mL) and TFA (30 mL) were added to Compound 8 (4 g, crude) and the reaction mixture stirred at RT for 22 h. The solution was concentrated and the residue dissolved in EtOAc, washed with 10% aq. NH₄OH, dried over Na₂SO₄ and concentrated. Purification by MPLC (0-15% MeOH/DCM) yielded 1.57 g of Compound 23 as a brown foam and 1.01 g of Compound 24 as a brown oil.

Compound 23 LC/MS, m/z=399 [M+H]⁺ (Calc: 398).

Compound 24 LC/MS, m/z=399 [M+H]⁺ (Calc: 398).

Example 10 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carbonitrile (25) (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carbonitrile (26)

(a) TFAA (0.21 mL, 1.5 mmol) was added dropwise to Compound 23 (199 mg, 0.5 mmol) and DIPEA (0.26 mL, 1.5 mmol) in 2 mL THF. The solution was stirred at RT for 2.75 h and then concentrated. MeOH (2 mL) was added followed by 2.5M aq. NaOH (0.6 mL, 1.5 mmol) and the reaction heated at reflux for 4 h. EtOAc was added, washed with satd. aq. NaHCO₃, dried over Na₂SO₄, and concentrated. The resulting material was purified by preparatory HPLC [0-60% ACN/H₂O (0.01% TFA)] to yield Compound 25 as its TFA salt.

Compound 25 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.78 (bs, 1H), 7.06 (d, J=8.8 Hz, 1H), 7.00 (d, J=2.4 Hz, 1H), 6.80 (dd, J=8.8, 2.4 Hz, 1H), 6.05 (s, 1H), 4.05 (d, J=5.2 Hz, 1H), 3.69 (s, 3H), 3.37-3.15 (m, 4H), 2.94 (d, J=6.8 Hz, 1H), 2.85-2.76 (m, 1H), 2.69 (d, J=13.1 Hz, 1H), 2.36-2.13 (m, 4H), 1.77 (dd, J=13.1, 8.1 Hz, 1H), 1.50 (d, J=9.1 Hz, 1H), 1.05-0.92 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.48 (m, 1H), 0.44-0.30 (m, 2H); LC/MS, m/z=339 [M+H]⁺ (Calc: 338).

(b) Compound 26 was prepared analogously from Compound 24.

Compound 26 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.89 (bs, 1H), 7.10 (d, J=8.4 Hz, 1H), 6.94 (d, J=2.2 Hz, 1H), 6.84 (dd, J=8.4, 2.2 Hz, 1H), 6.26 (s, 1H), 4.11 (d, J=4.9 Hz, 1H), 3.75 (s, 3H), 3.39-3.26 (m, 2H), 3.19 (dd, J=20.4, 6.3 Hz, 1H), 3.05-2.81 (m, 4H), 2.40-2.20 (m, 3H), 2.11-1.99 (m, 2H), 1.55 (d, J=11.9 Hz, 1H), 1.12-1.00 (m, 1H), 0.72-0.64 (m, 1H), 0.64-0.55 (m, 1H), 0.51-0.36 (m, 2H); LC/MS, m/z=339 [M+H]⁺ (Calc: 338).

Example 11 (2S,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a-ol (28) (2S,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a,8-diol (29)

(a) (Bis(trifluoroacetoxy)iodo)benzene (1.51 g, 3.5 mmol) was added to Compound 24 (0.7 g, 1.76 mmol) in 10 mL 1:1 ACN:H₂O and the solution stirred at RT for 46 h. Another aliquot of (bis(trifluoroacetoxy)iodo)benzene (1.51 g, 3.5 mmol) was added and the reaction stirred for an additional 8 days 20 h. A 10% aq. solution of HCl was added and washed with Et₂O. The aqueous layer was basified with 10% aq. NH₄OH to pH 10-11, and extracted with EtOAc. The EtOAc layer was dried over Na₂SO₄, and concentrated to yield 480 mg of Compound 27 as a yellow oil. LC/MS, m/z=371 [M+H]⁺ (Calc: 370).

(b) AcOH (8 drops) was added to Compound 27 (190 mg, 0.5 mmol) in 2 mL MeOH and the solution heated at reflux for 24 h and stirred at RT until the total time stirring was 3.5 days. The reaction mixture was diluted with EtOAc, washed with 10% aq. NH₄OH, dried over Na₂SO₄, and concentrated to yield 220 mg of crude Compound 28 as a brown oil. A portion was purified by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield the title Compound 28 as its bis-TFA salt.

Compound 28 bis TFA salt: ¹H NMR (DMSO-d₆) δ: 8.87 (bs, 1H), 7.96 (bs, 3H), 7.11 (d, J=8.3 Hz, 1H), 6.97 (d, J=2.2 Hz, 1H), 6.87 (dd, J=8.3, 2.2 Hz, 1H), 6.23 (s, 1H), 4.18 (d, J=4.8 Hz, 2H), 3.75 (s, 3H), 3.38-3.19 (m, 3H), 3.02 (d, J=12.3 Hz, 1H), 2.94-2.80 (m, 2H), 2.42-2.19 (m, 2H), 2.11-2.02 (m, 2H), 1.85 (dd, J=14.9, 2.2 Hz, 1H), 1.57 (d, J=12.7 Hz, 1H), 1.10-1.00 (m, 1H), 0.73-0.64 (m, 1H), 0.64-0.56 (m, 2H), 0.51-0.37 (m, 2H); LC/MS, m/z=329 [M+H]⁺ (Calc: 328).

(c) Compound 29 was synthesized from Compound 28 as described in Example 8 and isolated as its bis-TFA salt.

Compound 29 his TFA salt: ¹H NMR (DMSO-d₆) δ: 9.45 (s, 1H), 8.82 (bs, 1H), 7.94 (bs, 3H), 6.99 (d, J=8.1 Hz, 1H), 6.77 (d, J=2.3 Hz, 1H), 6.68 (dd, J=8.1, 2.3 Hz, 1H), 6.16 (s, 1H), 4.15 (d, J=5.0 Hz, 1H), 3.33-3.13 (m, 4H), 3.01 (d, J=11.2 Hz, 1H), 2.93-2.84 (m, 1H), 2.71 (dd, J=12.0, 7.0 Hz, 1H), 2.31-2.23 (m, 2H), 2.13-2.01 (m, 2H), 1.83 (dd, J=15.1, 2.7 Hz, 1H), 1.51 (d, J=11.2 Hz, 1H), 1.10-0.99 (m, 1H), 0.72-0.64 (m, 1H), 0.64-0.55 (m, 1H), 0.51-0.36 (m, 2H); LC/MS, m/z=315 [M+H]⁺ (Calc: 314).

Example 12 (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (30)

A 2.5M aq. NaOH solution (5.4 mL, 13.5 mmol) was added to Compound 24 (1.79 g, 4.5 mmol) in 25 mL MeOH. The solution was heated at 80° C. for 1.75 h then concentrated. EtOAc was added, washed with satd. aq. NaHCO₃, dried over Na₂SO₄, and concentrated. DCM (10 mL) was added followed by the slow addition of a 1M solution of BBr₃ in DCM (18 mL, 18 mmol) at 0° C. The solution was stirred at 0° C. for 1 h, quenched with 7M NH₃/MeOH followed by MeOH and concentrated. DCM was added and washed with 10% aq. NH₄OH. The resulting sludge was decanted off and the organic layer was dried over Na₂SO₄ and concentrated. Purification by MPLC (0-15% (10% NH₄OH/MeOH)/DCM) yielded 330 mg of the title Compound 30 as a brown solid.

¹H NMR (DMSO-d₆) δ: 9.06 (s, 1H), 7.06 (bs, 1H), 6.87 (d, J=8.4, 1H), 6.72-6.66 (m, 2H), 6.52 (dd, J=8.4, 2.3, 1H), 4.56 (s, 1H), 3.22 (d, J=5.4, 1H), 2.96 (d, J=18.4, 1H), 2.68 (dd, J=18.0, 5.8, 1H), 2.57-2.52 (m, 1H), 2.42-2.21 (m, 4H), 2.11-1.96 (m, 2H), 1.81 (td, J=11.9, 3.1, 1H), 1.72 (d, J=6.9, 2H), 1.23 (d, J=11.9, 1H), 0.88-0.76 (m, 2H), 0.52-0.41 (m, 2H), 0.15-0.03 (m, 2H); LC/MS, m/z=343 [M+H]⁺ (Calc: 342).

Example 13 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (31)

To Compound 9 (1.66 g, 3.6 mmol, mixture of diastereomers) was added a 1M solution of BBr₃ in DCM (13.5 mL, 13.5 mmol) at 0° C. The solution was stirred at RT and after 2 h and 6 h additional aliquots of a 1M solution of BBr₃ in DCM (13.5 mL, 13.5 mmol) were added. After 22.5 h the reaction was slowly quenched with MeOH and concentrated. DCM (10 mL) followed by TFA (10 mL) was added and the solution stirred at RT for 90 min. Concentration followed by MPLC purification (0-15% MeOH/DCM) followed by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] yielded the title Compound 31 as its TFA salt.

Compound 31 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.20 (s, 1H), 8.67 (br. s, 1H), 7.05 (br. s., 1H), 6.93 (d, J=8.4 Hz, 1H), 6.66 (d, J=2.2 Hz, 1H), 6.55-6.62 (m, 2H), 5.69-5.82 (m, 1H), 4.04 (d, J=4.0 Hz, 1H), 3.21-3.33 (m, 3H), 2.90-3.18 (m, 4H), 2.75-2.90 (m, 1H), 2.15-2.36 (m, 3H), 1.90 (d, J=8.8 Hz, 2H), 1.42 (d, J=10.6 Hz, 1H), 1.08 (br. s., 1H), 0.62-0.72 (m, 1H), 0.52-0.62 (m, 1H), 0.44-0.52 (m, 1H), 0.36-0.44 (m, 1H); LC/MS, m/z=343 [M+H]⁺ (Calc: 342).

Example 14 (2R,3aS,4R,9bS)-N-benzyl-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (33) (2S,3aS,4R,9bS)-N-benzyl-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (34)

Rh₂(OAc)₄ (8 mg, 0.02 mmol) was added to a solution of Compound 2 (409 mg, 1 mmol) and benzylamine (129 mg, 1.2 mmol) in 5 mL toluene. The solution was heated at reflux for 16 h and concentrated. MeOH (5 mL) and AcOH (4 drops) were added and the solution heated at 60° C. for 20 h. Concentration followed by MPLC purification (0-15% MeOH/DCM) yielded 297 mg of Compound 32 as a brown oil (mixture of amide diasteromers). DCM (4 mL) was added followed by a 1M solution of BBr₃ in DCM (2.7 mL, 2.7 mmol) and the mixture stirred at RT for 90 min. The reaction was quenched with 7M NH₃/MeOH and concentrated. The resulting material was purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) followed by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield Compound 33 and Compound 34 as their TFA salts.

Compound 33 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.19 (bs, 1H), 8.65 (bs, 1H), 8.07 (t, J=5.9 Hz, 1H), 7.21 (t, J=7.6 Hz, 2H), 7.13 (t, J=6.9 Hz, 1H), 6.99 (d, J=6.9 Hz, 1H), 6.89 (d, J=8.4 Hz, 1H), 6.63 (d, J=2.0 Hz, 1H), 6.55 (dd, J=8.4, 2.0 Hz, 1H), 5.77 (s, 1H), 4.08 (d, J=5.2 Hz, 2H), 4.00 (d, J=4.4 Hz, 1H), 3.30-3.16 (m, 2H), 2.91 (d, J=10.1 Hz, 1H), 2.80-2.70 (m, 1H), 2.51 (dd, J=13.3, 2.9 Hz, 1H), 2.30-2.11 (m, 3H), 1.89 (d, J=7.8 Hz, 2H), 1.38 (d, J=11.3 Hz, 1H), 1.06-0.95 (m, 1H), 0.65-0.56 (m, 1H), 0.56-0.48 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.30 (m, 1H). LC/MS, m/z=433 [M+H]⁺ (Calc: 432).

Compound 34 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (bs, 1H), 8.75-8.63 (m, 2H), 7.30-7.24 (m, 2H), 7.22-7.15 (m, 3H), 6.92 (d, J=8.1 Hz, 1H), 6.71 (d, J=2.0 Hz, 1H), 6.59 (dd, J=8.1, 2.0 Hz, 1H), 6.53 (s, 1H), 4.24 (d, J=6.6 Hz, 2H), 4.04 (d, J=4.9 Hz, 1H), 3.31-3.18 (m, 2H), 3.09 (dd, J=18.9, 5.8 Hz, 1H), 2.92 (d, J=10.7 Hz, 1H), 2.81-2.72 (m, 1H), 2.70-2.52 (m, 2H), 2.35-2.15 (m, 2H), 1.94 (dd, J=12.4, 8.2 Hz, 1H), 1.80 (d, J=6.6 Hz, 2H), 1.42 (d, J=11.5 Hz, 1H), 1.06-0.94 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.48 (m, 1H), 0.44-0.29 (m, 1H). LC/MS, m/z=433 [M+H]⁺ (Calc: 432).

The following compounds were prepared in an analogous fashion. Purification was done via preparatory HPLC [0-60% ACN/H₂O (0.01% TFA)] or preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)]:

(a) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 35) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.19 (bs, 1H), 8.72 (s, 1H), 7.59 (t, J=5.6 Hz, 1H), 6.93 (d, J=8.4 Hz, 1H), 6.64 (d, J=2.2 Hz, 1H), 6.58 (dd, J=8.4, 2.2 Hz, 1H), 5.82 (s, 1H), 4.05 (d, J=3.9 Hz, 1H), 3.35-3.22 (m, 2H), 3.17-2.92 (m, 3H), 2.87-2.66 (m, 3H), 2.47 (dd, J=13.4, 2.8 Hz, 1H), 2.36-2.16 (m, 2H), 1.91 (d, J=8.4 Hz, 2H), 1.56 (sept., J=6.7 Hz, 1H), 2.43 (d, J=11.2 Hz, 1H), 1.14-1.02 (m, 1H), 0.74 (dd, J=6.7, 4.4 Hz, 6H), 0.71-0.63 (m, 1H), 0.63-0.53 (m, 1H), 0.52-0.45 (m, 1H), 0.44-0.31 (m, 1H); LC/MS, m/z=399 [M+H]⁺ (Calc: 398).

(b) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 36) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.30 (bs, 1H), 8.68 (bs, 1H), 8.18 (t, J=5.5 Hz, 1H), 6.91 (d, J=8.2 Hz, 1H), 6.75-6.66 (m, 2H), 6.59 (dd, J=8.2, 2.1 Hz, 1H), 4.04 (d, J=4.8 Hz, 1H), 3.31-3.18 (m, 2H), 3.07 (dd, J=19.3, 5.5 Hz, 1H), 2.96-2.72 (m, 4H), 2.68-2.49 (m, 2H), 2.34-2.14 (m, 2H), 1.90-1.79 m, 1H), 1.79-1.67 (m, 2H), 1.62 (sept., J=6.2 Hz, 1H), 1.42 (d, J=10.3 Hz, 1H), 1.06-0.94 (m, 1H), 0.78 (d, 6.2 Hz, 6H), 0.65-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.44-0.36 (m, 1H), 0.36-0.29 (m, 1H); LC/MS, m/z=399 [M+H]⁺ (Calc: 398).

(c) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 37) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.26 (bs, 1H), 8.72 (bs, 1H), 8.10 (t, J=6.4 Hz, 1H), 7.09 (d, J=7.9 Hz, 2H), 6.98-6.97 (m, 3H), 6.70 (d, J=2.6 Hz, 1H), 6.62 (dd, J=8.3, 2.6 Hz, 1H), 5.83 (s, 1H), 4.10 (d, J=6.4 Hz, 2H), 4.06 (d, J=4.9 Hz, 1H), 3.30-3.24 (m, 2H), 3.18-3.06 (m, 2H), 2.99 (d, J=9.1 Hz, 1H), 2.86-2.77 (m, 1H), 2.56 (dd, J=13.3, 2.8 Hz, 1H), 2.37-2.18 (m, 6H), 1.95 (d, J=8.4 Hz, 2H), 1.45 (d, J=11.2 Hz, 1H), 1.13-1.03 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.54 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=447 [M+H]⁺ (Calc: 446).

(d) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 38) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.36 (bs, 1H), 8.75 (bs, 1H), 8.71 (t, J=5.7 Hz, 1H), 7.16-7.11 (m, 4H), 6.99 (d, J=8.4 Hz, 1H), 6.78 (d, J=2.3 Hz, 1H), 6.66 (dd, J=8.4, 2.3 Hz, 1H), 6.64 (bs, 1H), 4.26 (d, J=5.7 Hz, 2H), 4.11 (d, J=4.4 Hz, 1H), 3.38-3.26 (m, 2H), 3.16 (dd, J=19.6, 5.8 Hz, 1H), 2.99 (d, J=9.4 Hz, 1H), 2.88-2.79 (m, 2H), 2.76-2.66 (m, 1H), 2.62 (dd, J=12.3, 8.0 Hz, 1H), 2.42-2.24 (m, 5H), 1.99 (dd, J=12.3, 8.0 Hz, 1H), 1.90-1.82 (m, 2H), 1.49 (d, J=10.9 Hz, 1H), 1.13-1.02 (m, 1H), 0.73-0.65 (m, 1H), 0.63-0.55 (m, 1H), 0.51-0.44 (m, 1H), 0.44-0.36 (m, 1H); LC/MS, m/z=447 [M+H]⁺ (Calc: 446).

(e) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 39) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.25 (bs, 2H), 8.72 (bs, 1H), 8.02 (t, J=5.7 Hz, 1H), 6.95 (d, J=8.6 Hz, 1H), 6.88 (d, J=7.7 Hz, 2H), 6.71-6.65 (m, 3H), 6.62 (dd, J=8.5, 2.1 Hz, 1H), 5.82 (s, 1H), 4.06 (d, J=3.5 Hz, 1H), 4.03 (d, J=5.7 Hz, 2H), 3.36-3.24 (m, 2H), 3.17-3.04 (m, 2H), 2.98 (d, J=9.8 Hz, 1H), 2.86-2.77 (m, 1H), 2.54 (dd, J=11.2, 2.8 Hz, 1H), 2.36-2.16 (m, 3H), 1.94 (d, J=8.4 Hz, 2H), 1.44 (d, J=11.2 Hz, 1H), 1.13-1.02 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=449 [M+H]⁺ (Calc: 448).

(f) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 40) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.36 (bs, 1H), 9.34 (bs, 1H), 8.76 (bs, 1H), 8.56 (t, J=5.7 Hz, 1H), 7.05 (d, J=8.3 Hz, 2H), 6.98 (d, J=8.3 Hz, 1H), 6.77 (d, J=2.4 Hz, 1H), 6.74-6.69 (m, 3H), 6.66 (dd, J=8.3, 2.4 Hz, 1H), 4.19 (dd, J=5.7 Hz, 2H), 4.11 (d, J=5.2 Hz, 1H), 3.38-3.26 (m, 2H), 3.15 (dd, J=19.3, 5.9 Hz, 1H), 2.99 (d, J=11.1 Hz, 1H), 2.88-2.79 (m, 1H), 2.75-2.66 (m, 1H), 2.61 (dd, J=11.9, 8.1 Hz, 1H), 2.42-2.20 (m, 2H), 1.97 (dd, J=11.9, 8.1 Hz, 1H), 1.88-1.80 (m, 2H), 1.49 (d, J=10.4 Hz, 1H), 1.13-1.02 (m, 1H), 0.73-0.64 (m, 1H), 0.64-0.55 (m, 1H), 0.51-0.44 (m, 1H), 0.44-0.36 (m, 1H); LC/MS, m/z=449 [M+H]⁺ (Calc: 448).

(g) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 41) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.32 (s, 1H), 9.24 (bs, 1H), 9.07 (bs, 1H), 8.71 (bs, 1H), 8.00 (t, J=5.8 Hz, 1H), 6.95 (s, J=8.2 Hz, 1H), 6.68 (d, J=2.2 Hz, 1H), 6.66 (d, J=8.3 Hz, 1H), 6.61 (dd, J=8.3, 2.2 Hz, 1H), 6.22 (d, J=2.4 Hz, 1H), 6.14 (dd, J=8.3, 2.2 Hz, 1H), 5.81 (s, 1H), 4.06 (d, J=3.7 Hz, 1H), 3.96 (t, J=4.8 Hz, 2H), 3.35-3.23 (m, 2H), 3.19-3.06 (m, 2H), 2.97 (d, J=10.3 Hz, 1H), 2.86-2.77 (m, 1H), 2.57-2.52 (m, 1H), 2.36-2.16 (m, 3H), 1.93 (d, J=8.8 Hz, 2H), 1.44 (d, J=11.0 Hz, 1H), 1.15-1.02 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.53-0.45 (m, 1H), 0.43-0.37 (m, 1H); LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

(h) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 42) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.41 (s, 1H), 9.36 (s, 1H), 9.16 (s, 1H), 8.76 (bs, 1H), 8.50 (t, J=5.4 Hz, 1H), 6.98 (d, J=8.4 Hz, 1H), 6.86 (d, J=8.4 Hz, 1H), 6.82 (s, 1H), 6.77 (d, J=2.1 Hz, 1H), 6.66 (dd, J=8.4, 2.1 Hz, 1H), 6.28 (d, J=2.1 Hz, 1H), 6.17 (dd, J=8.4, 2.1 Hz, 1H), 4.17-4.06 (m, 3H), 3.37-3.26 (m, 2H), 3.15 (dd, J=18.9, 5.5 Hz, 1H), 2.99 (d, J=10.2 Hz, 1H), 2.88-2.79 (m, 1H), 2.79-2.70 (m, 1H), 2.61 (dd, J=12.6, 7.9 Hz, 1H), 2.42-2.22 (m, 2H), 1.95 (dd, J=12.6, 7.9 Hz, 1H), 1.87-1.77 (m, 2H), 1.49 (d, J=11.8 Hz, 1H), 1.13-1.02 (m, 1H), 0.73-0.65 (m, 1H), 0.63-0.55 (m, 1H), 0.51-0.44 (m, 1H), 0.44-0.36 (m, 1H); LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

(i) (2R,3aS,4R,9bS)-N-(4-chlorobenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 43) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (bs, 1H), 8.73 (bs, 1H), 8.18 (t, J=5.9 Hz, 1H), 7.33 (d, J=8.5 Hz, 2H), 7.06 (d, J=8.5 Hz, 2H), 6.96 (d, J=8.5 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.63 (dd, J=8.5, 2.3 Hz, 1H), 5.85 (s, 1H), 4.14 (t, J=5.1 Hz, 2H), 4.07 (d, J=4.4 Hz, 1H), 3.36-3.24 (m, 2H), 3.19-3.07 (m, 2H), 2.99 (d, J=9.5 Hz, 1H), 2.87-2.77 (m, 1H), 2.58 (d, J=13.2 Hz, 1H), 2.36-2.18 (m, 3H), 1.96 (d, J=8.8 Hz, 2H), 1.45 (d, J=10.3 Hz, 1H), 1.13-1.03 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=467 [M+H]⁺ (Calc: 466).

(j) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 44) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.30 (bs, 1H), 8.74 (bs, 1H), 8.21 (t, J=6.0 Hz, 1H), 7.57 (d, J=2.0 Hz, 1H), 7.36 (dd, J=8.0, 2.0 Hz, 1H), 6.98-6.92 (m, 2H), 6.71 (d, J=2.0 Hz, 1H), 6.63 (dd, J=8.0, 2.0 Hz, 1H), 5.88 (s, 1H), 4.16 (d, J=6.0 Hz, 2H), 4.08 (d, J=3.9 Hz, 1H), 3.37-3.24 (m, 2H), 3.22-3.07 (m, 2H), 2.99 (d, J=9.4 Hz, 1H), 2.87-2.77 (m, 1H), 2.61 (dd, J=13.4, 2.8 Hz, 1H), 2.37-2.18 (m, 3H), 1.97 (d, J=9.5 Hz, 2H), 1.46 (d, J=10.3 Hz, 1H), 1.14-1.02 (m, 1H), 0.73-0.63 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

(k) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 45) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.39 (bs, 1H), 8.76 (bs, 1H), 8.70 (t, J=5.9 Hz, 1H), 7.63 (d, J=1.8 Hz, 1H), 7.44 (dd, J=8.3, 1.8 Hz, 1H), 7.33 (d, J=8.3 Hz, 1H), 6.99 (d, J=8.3 Hz, 1H), 6.79 (d, J=2.2 Hz, 1H), 6.67 (dd, J=8.3, 2.2 Hz, 1H), 6.37 (s, 1H), 4.40-4.29 (m, 2H), 4.11 (d, J=4.8 Hz, 1H), 3.37-3.26 (m, 2H), 3.16 (dd, J=19.2, 5.6 Hz, 1H), 2.99 (d, J=9.6 Hz, 1H), 2.89-2.79 (m, 1H), 2.78-2.67 (m, 1H), 2.62 (dd, J=12.8, 8.8 Hz, 1H), 2.41-2.23 (m, 2H), 2.02 (dd, J=12.8, 8.8 Hz, 1H), 1.94-1.85 (m, 2H), 1.49 (d, J=11.2 Hz, 1H), 1.13-1.01 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.51-0.43 (m, 1H), 0.43-0.35 (m, 1H); LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

(l) (2R,3 aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 46) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.27 (s, 1H), 8.73 (bs, 1H), 8.23 (t, J=6.1 Hz, 1H), 7.53 (d, J=8.4 Hz, 1H), 7.39 (d, J=1.9 Hz, 1H), 6.99 (dd, J=8.4, 1.9 Hz, 1H), 6.95 (d, J=8.4 Hz, 1H), 6.68 (d, J=2.3 Hz, 1H), 6.61 (dd, J=8.4, 2.3 Hz, 1H), 5.86 (s, 1H), 4.14 (d, J=6.1 Hz, 2H), 4.07 (d, J=3.9 Hz, 1H), 3.34-3.23 (m, 2H), 3.18-3.07 (m, 2H), 2.98 (d, J=10.0 Hz, 1H), 2.86-2.77 (m, 1H), 2.56 (dd, 13.1, 2.3 Hz, 1H), 2.37-2.17 (m, 3H), 2.01-1.88 (m, 2H), 1.45 (d, 11.6, 1H), 1.13-1.02 (m, 1H), 0.72-0.64 (m, 1H( ), 0.63-0.55 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.36 (m, 1H); LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

(m) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 47) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.36 (s, 1H), 8.80-8.69 (m, 2H), 7.61 (d, J=8.4 Hz, 1H), 7.50 (d, J=1.8 Hz, 1H), 7.25 (dd, J=8.4, 1.8 Hz, 1H), 6.99 (d, J=8.1 Hz, 1H), 6.78 (d, J=2.2 Hz, 1H), 6.67 (dd, J=8.1, 2.2 Hz, 1H), 6.39 (s, 1H), 4.30 (d, J=6.2 Hz, 2H), 4.11 (d, J=4.1 Hz, 1H), 3.37-3.24 (m, 2H), 3.16 (dd, J=20.2, 6.2 Hz, 1H), 2.99 (d, J=9.3 Hz, 1H), 2.88-2.78 (m, 1H), 2.74-2.57 (m, 2H), 2.41-2.22 (m, 2H), 2.02 (dd, J=11.7, 7.8 Hz, 1H), 1.92-1.85 (m, 2H), 1.49 (d, J=11.7 Hz, 1H), 1.12-1.01 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.51-0.44 (m, 1H), 0.44-0.36 (m, 1H); LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

(n) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(4-fluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 48) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (bs, 1H), 8.73 (bs, 1H), 8.16 (t, J=5.9 Hz, 1H), 7.14-7.04 (m, 4H), 6.96 (d, J=8.5 Hz, 1H), 6.70 (d, J=2.1 Hz, 1H), 6.63 (dd, J=8.5, 2.1 Hz, 1H), 5.85 (s, 1H), 4.13 (d, J=5.9 Hz, 1H), 4.07 (d, J=4.3 Hz, 1H), 3.36-3.24 (m, 2H), 3.18-3.07 (m, 2H), 2.99 (d, J=10.4 Hz, 1H), 2.87-2.77 (m, 1H), 2.57 (dd, J=13.2, 2.9 Hz, 1H), 2.37-2.17 (m, 3H), 1.96 (d, J=8.9 Hz, 2H), 1.45 (d, J=11.6 Hz, 1H), 1.13-1.03 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=451 [M+H]⁺ (Calc: 450).

(o) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,5-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 49) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.20 (bs, 1H), 8.74 (bs, 1H), 8.25 (t, J=5.9 Hz, 1H), 7.06 (tt, J=9.3, 2.3 Hz, 1H), 6.74 (d, J=8.4 Hz, 1H), 6.85-6.78 (m, 2H), 6.67 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.4, 2.3 Hz, 1H), 5.86 (s, 1H), 4.16 (dd, J=9.0, 5.9 Hz, 2H), 4.07 (d, J=4.5 Hz, 1H), 3.35-3.32 (m, 2H), 3.19-3.07 (m, 2H), 2.98 (d, J=10.8 Hz, 1H), 2.87-2.78 (m, 1H), 2.56 (dd, J=12.8, 2.8 Hz, 1H), 2.37-2.18 (m, 3H), 2.04-1.87 (m, 2H), 1.45 (d, J=11.2 Hz, 1H), 1.13-1.03 (m, 1H), 0.72-0.63 (m, 1H), 0.63-0.54 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.36 (m, 1H); LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

(p) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 50) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (s, 1H), 8.71 (bs, 1H), 7.70 (t, J=5.3 Hz, 1H), 7.27 (t, J=7.1 Hz, 2H), 7.22-7.16 (m, 1H), 7.10 (d, J=7.1 Hz, 2H), 6.95 (d, J=8.3 Hz, 1H), 6.67 (d, J=2.4 Hz, 1H), 6.60 (dd, J=8.3, 2.4 Hz, 1H), 5.82 (s, 1H), 4.05 (d, J=4.8 Hz, 1H), 3.35-3.24 (m, 2H), 3.16-3.06 (m, 3H), 3.06-2.92 (m, 2H), 2.86-2.77 (m, 1H), 2.59 (t, J=6.9 Hz, 2H), 2.35-2.13 (m, 3H), 1.89 (d, J=8.3 Hz, 2H), 1.43 (d, J=9.6 Hz, 1H), 1.13-1.03 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=447 [M+H]⁺ (Calc: 446).

(q) (2S,3 aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 51) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.38 (bs, 1H), 8.75 (bs, 1H), 8.36 (t, J=5.7 Hz, 1H), 7.33-7.26 (m, 2H), 2.24-7.17 (m, 3H), 6.98 (d, J=8.3 Hz, 1H), 6.76 (d, J=2.2 Hz, 1H), 6.70 (bs, 1H), 6.66 (dd, J=8.3, 2.2 Hz, 1H), 4.10 (d, J=5.6 Hz, 1H), 3.40-3.23 (m, 4H), 3.14 (dd, J=19.7, 5.6 Hz, 1H), 2.99 (d, J=9.8 Hz, 1H), 2.88-2.79 (m, 1H), 2.73 (t, J=7.0 Hz, 2H), 2.68-2.52 (m, 2H), 2.40-2.22 (m, 4H), 1.88 (dd, J=12.9, 8.3 Hz, 1H), 1.83-1.72 (m, 2H), 1.48 (d, J=11.1 Hz, 1H), 1.13-1.01 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.51-0.43 (m, 1H), 0.43-0.35 (m, 1H); LC/MS, m/z=447 [M+H]⁺ (Calc: 446).

(r) (2R,3 aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-3-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 89): LC/MS, m/z=434.2 [M+H]⁺ (Calc: 433.2).

(s) (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-4-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 90): LC/MS, m/z=434.2 [M+H]⁺ (Calc: 433.2).

(t) (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-3-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 92): LC/MS, m/z=434.2 [M+H]⁺ (Calc: 433.2).

Example 15 ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(piperidin-1-yl)methanone (56) ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(piperidin-1-yl)methanone (57)

Rh₂(OAc)₄ (8 mg, 0.02 mmol) was added to a solution of Compound 2 (409 mg, 1 mmol) and piperidine (102 mg, 1.2 mmol) in 5 mL toluene. The solution was heated at reflux for 16 h and concentrated. MeOH (5 mL) and AcOH (4 drops) were added and the solution heated at 60° C. for 20 h. Concentration followed by MPLC purification (0-15% MeOH/DCM) yielded 141 mg of Compound 52 as a brown oil and Compound 53. MeOH (2 mL) and AcOH (5 drops) were added to Compound 52 and the solution heated at reflux for 3.5 days and concentrated to yield 109 mg of Compound 54 as a brown oil. Similarly Compound 53 was converted to Compound 55. DCM (4 mL) was added separately to Compound 54 and Compound 55 followed by a 1M solution of BBr₃ in DCM (4 equiv.) and each mixture stirred at RT for 90 min Each reaction was quenched with 7M NH₃/MeOH and concentrated. The resulting material from Compound 54 was purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield the title Compound 56 as its HBr salt. The resulting material from Compound 55 was purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) followed by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield the title Compound 57 as its TFA salt.

Compound 56 HBr salt: ¹H NMR (DMSO-d₆/DCl) δ: 6.89 (d, J=8.3 Hz, 1H, partially obscurred by DCl), 6.63 (d, J=2.1 Hz, 1H), 6.56 (dd, J=8.3, 2.1 Hz, 1H), 4.09 (d, J=5.2 Hz, 1H), 3.43-3.15 (m, 7H), 3.07 (dd, J=19.1, 5.2 Hz, 1H), 2.94 (dd, J=12.5, 3.5 Hz, 1H), 2.81 (dd, J=13.2, 7.3 Hz, 1H), 2.39-2.09 (m, 4H), 1.98-1.85 (m, 2H), 1.57-1.21 (m, 7H), 1.08-0.98 (m, 1H), 0.66-0.57 (m, 1H), 0.57-0.42 (m, 2H), 0.39-0.31 (m, 1H). LC/MS, m/z=411 [M+H]⁺ (Calc: 410).

Compound 57 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.12 (bs, 1H), 8.49 (bs, 1H), 6.74 (d, J=8.1 Hz, 1H), 6.55 (d, J=2.5 Hz, 1H), 6.41 (dd, J=8.1, 2.5 Hz, 1H), 6.32 (s, 1H), 3.85 (d, J=5.6 Hz, 1H), 3.32-3.01 (m, 6H), 2.96-2.80 (m, 2H), 2.74 (d, J=10.6 Hz, 1H), 2.63-2.54 (m, 1H), 2.34 (dd, J=12.6, 8.6 Hz, 1H), 2.13-1.97 (m, 2H), 1.74 (dd, J=12.6, 8.6 Hz, 1H), 1.69-1.59 (m, 2H), 1.40-1.28 (m, 2H), 1.27-1.13 (m, 5H), 0.87-0.76 (m, 1H), 0.48-0.39 (m, 1H), 0.38-0.30 (m, 1H), 0.26-0.19 (m, 1H), 0.19-0.10 (m, 1H). LC/MS, m/z=411 [M+H]⁺ (Calc: 410).

The following compounds were prepared in an analogous manner:

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(morpholino)methanone (Compound 58) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.22 (s, 1H), 8.73 (bs, 1H), 6.94 (d, J=8.4 Hz, 1H), 6.68 (d, J=2.2 Hz, 1H), 6.58 (dd, J=8.4, 2.2 Hz, 1H), 5.83 (s, 1H), 4.07 (d, J=4.5 Hz, 1H), 3.68-3.32 (m, 12H, overlapping with H₂O), 3.14 (dd, J=19.1, 5.1 Hz, 1H), 2.97 (d, J=10.1 Hz, 1H), 2.86-2.76 (m, 1H), 2.44 (dd, J=13.0, 3.4 Hz, 1H), 2.36-2.18 (m, 3H), 2.06 (dd, J=13.0, 9.0 Hz, 1H), 1.88 (dd, J=13.0, 7.9 Hz, 1H), 1.45 (d, J=11.3 Hz, 1H), 1.12-1.01 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.52-0.45 (m, 1H), 0.45-0.36 (m, 1H). LC/MS, m/z=413 [M+H]⁺ (Calc: 412).

((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(thiomorpholino)-methanone (Compound 87): LC/MS, m/z=429.1 [M+H]⁺ (Calc: 428.1).

((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(1,1-dioxido-thiomorpholino)methanone (Compound 94): LC/MS, m/z=461.2 [M+H]⁺ (Calc: 460.2).

(2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N,N-diethyl-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (Compound 59) TFA salt: ¹H NMR (DMSO-d₆) δ: 9.18 (s, 1H), 8.74 (bs, 1H), 6.94 (d, J=7.9 Hz, 1H), 6.63 (d, J=2.1 Hz, 1H), 6.58 (dd, J=7.9, 2.1 Hz, 1H), 5.88 (s, 1H), 4.07 (d, J=4.8 Hz, 1H), 3.43-3.19 (m, 7H, overlapping with H₂O), 3.13 (dd, J=18.7, 5.4 Hz, 1H), 3.09-2.93 (m, 2H), 2.86-2.77 (m, 1H), 2.40-2.17 (m, 4H), 2.06 (dd, J=13.9, 10.9 Hz, 1H), 1.92-1.83 (m, 1H), 1.43 (d, J=11.5 Hz, 1H), 1.13 (t, J=7.2 Hz, 3H), 1.10-1.03 (m, 1H), 0.91 (t, J=7.2 Hz, 3H), 0.72-0.63 (m, 1H), 0.63-0.54 (m, 1H), 0.54-0.46 (m, 1H), 0.45-0.37 (m, 1H). LC/MS, m/z=399 [M+H]⁺ (Calc: 398).

Example 16 (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N,N-dimethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (62) (2R,3aS,4R,9bS)-N-(2-amino-2-oxoethyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (64) 2-((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamido)acetic acid (66)

(a) Two drops of DMF were added to Compound 14 (1.42 g, 3.97 mmol) and oxalyl chloride (1.01 g, 7.95 mmol) in 20 mL DCM and the solution was stirred at RT for 2 h. The solution was concentrated to yield 1.46 g of Compound 60 as a brown solid that was carried on without further purification.

(b) Compound 60 (0.2 g, 0.53 mmol) was added to a 2M solution of dimethylamine in THF (1.33 mL, 2.66 mmol) and the mixture stirred at RT for 75 min DCM was added, washed with satd aq. NaHCO₃, passed through a phase separation column, and concentrated to yield 156 mg of Compound 61. Compound 61 was O-demethylated as described in Example 14 and purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield the title Compound 62 as its HBr salt: ¹H NMR (DMSO-d₆) δ: 9.23 (s, 1H), 8.76 (bs, 1H), 6.93 (d, J=8.3 Hz, 1H), 6.65 (s, 1H), 6.59 (d, J=8.3 Hz, 1H), 5.90 (s, 1H), 4.08 (s, 1H), 3.46-3.22 (m, 4H, overlapping with H₂O), 3.13 (dd, J=18.8, 4.2 Hz, 1H), 2.99 (s, 1H), 2.93-2.88 (m, 1H), 2.87-2.76 (m, 1H), 2.71 (s, 3H), 2.47-2.16 (m, 4H), 2.05-1.86 (m, 2H), 1.44 (d, J=12.5 Hz, 1H), 1.13-1.02 (m, 1H), 0.73-0.63 (m, 1H), 0.63-0.54 (m, 1H), 0.54-0.46 (m, 1H). 0.46-0.36 (m, 1H); LC/MS, m/z=371 [M+H]⁺ (Calc: 370).

(c) Compound 60 (0.2 g, 0.53 mmol) was added to glycinamide-HCl (71 mg, 0.63 mmol) and DIPEA (0.28 mL, 1.60 mmol) in 2 mL DCM and the solution stirred at RT for 75 min. DCM was added, washed with satd. aq. NaHCO₃, passed through a phase separation column, and concentrated to yield 144 mg of Compound 63. Compound 63 was O-demethylated as described in Example 14 and purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield the title Compound 64 as its HBr salt.

Compound 64 HBr salt: ¹H NMR (DMSO-d₆/DCl) δ: 6.89 (d, J=8.2 Hz, 1H), 6.62 (d, J=2.1 Hz, 1H), 6.55 (dd, J=8.2, 2.1 Hz, 1H), 4.10 (d, J=5.4 Hz, 1H), 3.45 (s, 2H), 3.26-2.99 (m, 5H), 2.94 (d, J=11.7 Hz, 1H), 2.82 (dd, J=13.4, 7.3 Hz, 1H), 2.44 (d, J=11.7 Hz, 1H), 2.27 (td, J=12.9, 4.5 Hz, 1H), 1.98 (dd, J=12.9, 8.4 Hz, 1H), 1.77 (dd, J=12.9, 9.5 Hz, 1H), 1.34 (d, J=11.9 Hz, 1H), 1.24 (dd, J=14.3, 6.4 Hz, 1H), 1.08-0.97 (m, 1H), 0.65-0.57 (m, 1H), 0.57-0.50 (m, 1H). 0.50-0.42 (m, 1H), 0.39-0.30 (m, 1H); LC/MS, m/z=400 [M+H]⁺ (Calc: 399).

(d) Compound 60 (0.2 g, 0.53 mmol) was added to sarcosine ethyl ester-HCl (98 mg, 0.63 mmol) and DIPEA (0.28 mL, 1.60 mmol) in 2 mL DCM and the solution stirred at RT for 75 min. DCM was added, washed with satd. aq. NaHCO₃, passed through a phase separation column, and concentrated to yield 206 mg of Compound 65. DCM (2 mL) was added followed by a 1M solution of BBr₃ in DCM (1.8 mL, 1.8 mmol) and the mixture stirred at RT for 90 min. The reaction was quenched with MeOH and concentrated. EtOH (2 mL) was added followed by 2.5M NaOH (0.9 mmol, 2.25 mmol) and the reaction mixture stirred at RT for 5 days followed by 1 h at reflux. The solution was concentrated and purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) followed by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] to yield the title Compound 66 as its TFA salt.

Compound 66 TFA salt: ¹H NMR (DMSO-d₆) δ: 12.96 (bs, 0.35H), 12.52 (bs, 0.65H), 9.25 (s, 0.35H), 9.19 (s, 0.65H), 8.72 (bs, 1H), 6.97-6.90 (m, 1H), 6.66-6.61 (m, 1H), 6.61-6.56 (m, 1H), 5.87 (s, 0.65H), 5.82 (s, 0.35H), 4.14 (d, J=7.3 Hz, 0.65H), 4.10-4.03 (m, 1H), 3.87 (dd, J=17.2, 23.1 Hz, 1.35H), 3.34-3.22 (m, 4H), 3.18-3.06 (m, 1H), 3.04 (s, 2H), 2.98 (d, J=11.2 Hz, 1H), 2.86-2.76 (m, 1H), 2.71 (s, 1H), 2.49-2.42 (m, 1H), 2.39-2.17 (m, 3H), 2.02-1.82 (m, 2H), 1.49-1.39 (m, 1H), 1.13-1.02 (m, 1H), 0.72-0.63 (m, 1H), 0.63-0.54 (m, 1H), 0.53-0.45 (m, 1H), 0.45-0.37 (m, 1H); LC/MS, m/z=415 [M+H]⁺ (Calc: 414).

Example 17 Preparation of Compounds 71-74

(a) Ac₂O (12.5 mL, 132 mmol) was added to Compound 67 (5.0 g, 13.25 mmol) and the solution heated at 120° C. for 24 h. The reaction mixture was diluted with EtOAc, washed with two portions of satd. aq. NaHCO₃, dried over Na₂SO₄, and concentrated. ACN (75 mL) and 4-acetamidobenzenesulfonyl azide (6.36 g, 26.5 mmol) were added, followed by DBU (6.0 mL, 39.7 mmol) at 0° C. The reaction was allowed to warm to RT over 18 h and 4-acetamidobenzenesulfonyl azide (3.1 g, 13.25 mmol) was added, followed by DBU (3 mL, 19.9 mmol). The reaction was stirred for an additional 3 days and concentrated. EtOAc was added, washed with two portions of 1M aq. NaOH, dried over Na₂SO₄ and concentrated. The resulting material was carried on without further purification to yield 7.47 g of Compound 68 as a brown foam. LC/MS, m/z=446 [M+H]⁺ (Calc: 445).

(b) Rh₂(OAc)₄ (117 mg, 0.27 mmol) was added to a solution of Compound 68 (5.90 g, 13.25 mmol) and 2,4-dimethoxybenzylamine (2.44 g, 14.6 mmol) in 75 mL toluene. The solution was heated at reflux for 15 h and concentrated to yield Compound 69 as a mixture of diastereomers. DCM (15 mL) followed by TFA (60 mL) were added and the solution stirred at RT for 27 h then concentrated. EtOAc was added, washed with two portions of 10% aq. NH₄OH, dried over Na₂SO₄, and concentrated. The crude material was purified by MPLC (0-10% MeOH/DCM) to yield 6.14 g of Compound 70 as a diastereomeric mixture that was carried on without further purification. LC/MS, m/z=435 [M+H]⁺ (Calc: 434).

(c) MeOH (32 mL) was added to Compound 70 (6.14 g, 13.25 mmol) followed by a 2.5M aq. NaOH solution (15.9 mL, 39.8 mmol). The reaction mixture was heated at 80° C. for 1 h and concentrated. EtOAc was added, washed with satd. aq. NaHCO₃, dried over Na₂SO₄, and concentrated. Purification by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) yielded 1.21 g of Compound 71 as a brown oil and 2.89 g of Compound 72 as a brown oil.

Compound 71: ¹H NMR (DMSO-d₆) δ: 7.40-7.35 (m, 2H), 7.35-7.29 (m, 2H), 7.28-7.22 (m, 1H), 7.01 (d, J=8.4 Hz, 1H), 6.96 (s, 1H), 6.76 (d, J=2.6 Hz, 1H), 6.66 (dd, J=8.4, 2.6 Hz, 1H), 6.49 (s, 1H), 4.35 (s, 1H), 3.75-3.59 (m, 5H), 3.22-3.14 (m, 1H), 3.01 (d, J=4.9 Hz, 1H), 3.00-2.90 (m, 1H), 2.69 (dd, J=18.1, 5.3 Hz, 1H), 2.53 (dd, J=12.3, 3.1 Hz, 1H), 2.38-2.30 (m, 1H), 2.12-1.97 (m, 2H), 1.86 (td, J=11.5, 3.1 Hz, 1H), 1.72-1.58 (m, 2H), 1.27 (d, J=12.3 Hz, 1H). LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

Compound 72: ¹H NMR (DMSO-d₆) δ: 7.40-7.35 (m, 2H), 7.35-7.30 (m, 2H), 7.28-7.21 (m, 1H), 7.08 (s, 1), 7.05 (d, J=8.3 Hz, 1H), 6.86 (d, J=2.8 Hz, 1H), 6.76-6.69 (m, 2H), 4.60 (s, 1H), 3.73 (s, 3H), 3.71-3.63 (m, 2H), 3.21-3.13 (m, 1H), 3.02 (d, J=5.5 Hz, 1H), 2.73 (dd, J=18.0, 5.5 Hz, 1H), 2.47 (dd, J=12.0, 7.8 Hz, 1H), 2.39-2.31 (m, 1H), 2.31-2.23 (m, 1H), 2.08-1.98 (m, 2H), 1.93-1.84 (m, 1H), 1.69 (d, J=7.8 Hz, 2H), 1.27 (d, J=12.0 Hz, 1H). LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

(d) AcOH (70 mL) was added to Compound 71 (2.89 g, 7.4 mmol). Pd(OH₂) on carbon (0.3 g, ˜10% w/w) was added and the reaction mixture shaken on the Parr shaker at 50 psi of H₂ for 69 h. The mixture was filtered through Celite, the solids washed with MeOH, and the filtrate concentrated. EtOAc was added to the resulting oil, washed with three portions of 10% aq. NH₄OH, dried over Na₂SO₄ and concentrated to yield a brown oil. The combined aqueous layers were concentrated, combined with the material from the organic layer, and purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield Compound 73 as a brown oil. LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

Compound 74 was prepared analogously from Compound 72. LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

Example 18 (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-(2,2,2-trifluoroethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (75) (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-(2,2,2-trifluoroethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (76)

(a) 2,2,2-Trifluoroethyl trifluoromethanesulfonate (154 mg, 0.66 mmol) was added to Compound 74 (0.2 g, 0.66 mmol) and DIPEA (0.35 mL, 1.98 mmol) in 3 mL ACN and the solution was heated at reflux. After 16 h 2,2,2-trifluoroethyl trifluoromethanesulfonate (383 mg, 1.65 mmol) was added and the solution heated at reflux for an additional 5 h. The reaction mixture was concentrated and the resulting material suspended in DCM (2 mL). A 1M solution of BBr₃ in DCM (2.65 mL, 2.65 mmol) was added and the mixture stirred at RT for 2 h. The solution was quenched by the addition of 1.5 mL 7M NH₃/MeOH and concentrated. Salts were removed by filtration and the resulting material was purified by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) to yield the title Compound 76.

Compound 76: ¹H NMR (DMSO-d₆) δ: 9.20 (bs, 1H), 7.43 (bs, 1H), 7.01 (bs, 1H), 6.91 (d, J=8.5 Hz, 1H), 6.72 (d, J=2.2 Hz, 1H), 6.59 (dd, J=8.5, 2.2 Hz, 1H), 3.76 (bs, 1H), 3.58 (bs, 1H), 3.34 (bs, 1H), 3.13-2.94 (m, 2H), 2.67 (bs 1H), 2.48-2.40 (m, 2H), 2.36-2.19 (m, 2H), 2.00-1.89 (m, 1H), 1.77-1.68 (m, 2H), 1.26 (d, J=11.0 Hz, 1H); LC/MS, m/z=371 [M+H]⁺ (Calc: 370).

(b) Compound 75 was prepared in an analogous manner:

Compound 75: ¹H NMR (DMSO-d₆) δ: 8.98 (bs, 1H), 6.92 (bs, 1H), 6.78 (d, J=8.3 Hz, 1H), 6.55 (d, J=2.4 Hz, 1H), 6.45 (bs, 1H), 6.43 (dd, J=8.3, 2.4 Hz, 1H), 3.48 (bs, 1H), 3.27 (bs, 1H), 3.12 (bs, 1H), 3.01-2.84 (m, 2H), 2.78 (d, J=18.4 Hz, 1H), 2.54-2.45 (m, 1H), 2.39 (dd, J=12.8, 3.3 Hz, 1H). 2.17-1.95 (m, 3H), 1.64 (d, J=9.2 Hz, 2H), 1.18 (d, J=11.4 Hz, 1H); LC/MS, m/z=371 [M+H]⁺ (Calc: 370).

Example 19 (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (77) (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide (78)

(a) Formaldehyde (37% in H₂O, 0.15 mL, 1.98 mmol) was added to Compound 74 (0.2 g, 0.66 mmol) in 3 mL DCM and the solution stirred at RT for 10 min. NaBH(OAc)₃ (280 mg, 1.32 mmol) was added and the solution stirred at RT for 4 h. DCM was added, washed with satd. aq. NaHCO₃, passed through a phase separation column, and concentrated. O-Demethylation was done as described in Example 17. Purification by MPLC (0-20% (10% NH₄OH/MeOH)/DCM) followed by preparatory HPLC [0-40% ACN/H₂O (0.01% TFA)] yielded the title Compound 78 as its TFA salt.

Compound 78 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.36 (s, 1H), 9.03 (bs, 1H, 7.71 (s, 1H), 7.25 (s, 1H), 6.99 (d, J=8.4 Hz, 1H), 6.76 (d, J=2.4 Hz, 1H), 6.66 (dd, J=8.4, 2.4 Hz, 1H), 6.58 (s, 1H), 3.78 (d, J=4.6 Hz, 1H), 3.30 (d, J=19.4 Hz, 1H), 3.11 (dd, J=19.4, 5.7 Hz, 1H), 2.99 (d, J=7.6 Hz, 1H), 2.80 (d, J=4.8 Hz, 3H), 2.69-2.55 (m, 2H), 2.36-2.24 (m, 2H), 1.98-1.89 (m, 2H), 1.83-1.76 (m, 2H), 1.47 (d, J=9.6 Hz, 1H).

LC/MS, m/z=303 [M+H]⁺ (Calc: 302).

(b) Compound 77 was prepared in an analogous manner from Compound 73.

Compound 77 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.24 (s, 1H), 9.02 (bs, 1H), 7.06 (s, 1H), 6.95 (d, J=8.1 Hz, 1H), 6.66 (d, J=2.5 Hz, 1H), 6.61 (s, 1H), 6.58 (dd, J=8.1, 2.5 Hz, 1H), 5.72 (s, 1H), 3.72 (d, J=4.7 Hz, 1H), 3.25 (d, J=19.3 Hz, 1H), 3.12-2.94 (m, 4H), 2.78 (d, J=4.7 Hz, 3H), 2.56-2.53 (m, 1H), 2.31-2.13 (m, 3H), 1.93-1.84 (m, 2H), 1.42 (d, J=9.1 Hz, 1H); LC/MS, m/z=303 [M+H]⁺ (Calc: 302).

Example 20 Preparation of Compounds 118, 119, 122, and 123

(a) N-Phenyl-bis(trifluoromethanesulfonimide) (4.45 g, 12.45 mmol) was added to Compound 0 (3.78 g, 8.3 mmol, prepared by a method that is analogous to those described above) and Cs₂CO₃ (8.11 g, 24.9 mmol) in 45 mL THF and the solution was stirred at 60° C. for 15 h. The reaction mixture was concentrated and DCM was added, washed with 1M NaOH, dried with Na₂SO₄ and concentrated. Purification by MPLC (0-10% MeOH/DCM) led to the isolation of Compound P as a yellow oil (2.88 g, 59%, mixture of amide diastereomers). LC/MS, m/z=588 [M+H]⁺ (Calc: 587).

(b) PdCl₂(dppf) (110 mg, 0.15 mmol) was added to a solution of Compound P (1.76 g, 3 mmol), zinc cyanide (211 mg, 1.8 mmol), and zinc dust (49 mg, 0.75 mmol) in 15 mL DMF and the solution was heated at 120° C. for 15.5 h. The solution was cooled and sparged with nitrogen for 5 min PdCl₂(dppf) (110 mg, 0.15 mmol) was added and the solution was heated at 120° C. for 8 h. PdCl₂(dppf) (110 mg, 0.15 mmol), zinc cyanide (211 mg, 1.8 mmol), and zinc dust (49 mg, 0.75 mmol) were added and the solution heated at 120° C. for 24 h. Additional PdCl₂(dppf) (110 mg, 0.15 mmol), zinc cyanide (211 mg, 1.8 mmol), and zinc dust (49 mg, 0.75 mmol) were added and the solution heated at 120° C. for 24 h. The reaction mixture was concentrated and purified by MPLC (0-20% MeOH/DCM) to yield Compound Q (1.88 g, 88%). A portion was purified by preparatory HPLC [0-40% MeCN/H₂O (0.01% TFA)] to yield Compound 122 and Compound 123 as their bis-TFA salts.

In a similar manner, the following compounds were synthesized:

Compound 84: LC/MS, m/z=456.3 [M+H]⁺ (Calc: 455.3).

Compound 85: LC/MS, m/z=470.2 [M+H]⁺ (Calc: 469.3).

Compound 91: LC/MS, m/z=470.2 [M+H]⁺ (Calc: 469.3).

Compound 93: LC/MS, m/z=456.20 [M+H]⁺ (Calc: 455.59).

Compound 122 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.71 (br. s., 1H), 8.84 (br. s., 1H), 8.05 (br. s., 1H), 7.86 (s, 1H), 7.64 (dd, J=8.0, 1.5 Hz, 1H), 7.34 (d, J=8.1 Hz, 1H), 6.04 (s, 1H), 4.15 (d, J=5.0 Hz, 1H), 3.97 (d, J=11.8 Hz, 2H), 3.67-3.18 (m, 7H, overlapped with water), 3.13-2.96 (m, 7H), 2.91-2.80 (m, 1H), 2.63 (d, J=13.5 Hz, 1H), 2.39-2.11 (m, 4H), 2.01-1.91 (m, 1H), 1.81 (dd, J=13.3, 8.6 Hz, 1H), 1.57 (d, J=12.5 Hz, 1H), 1.08 (br. s., 1H), 0.74-0.65 (m, 1H), 0.65-0.56 (m, 1H), 0.54-0.38 (m, 2H). LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

Compound 123 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.86 (br. s., 1H), 8.86 (br. s., 1H), 8.32 (br. s., 1H), 8.01 (s, 1H), 7.72 (dd, J=8.0, 1.5 Hz, 1H), 7.39 (d, J=8.1 Hz, 1H), 6.33 (br. s., 1H), 4.17 (d, J=4.8 Hz, 1H), 3.97 (br. s., 2H), 3.72-3.25 (m, 9H, overlapped with water), 3.24-3.06 (m, 5H), 3.02 (d, J=12.7 Hz, 1H), 2.93-2.84 (m, 1H), 2.76 (dd, J=12.6, 8.2 Hz, 1H), 2.42-2.31 (m, 1H), 2.29-2.14 (m, 1H), 2.08-2.02 (m, 1H), 1.95 (d, J=13.1 Hz, 1H), 1.84-1.73 (m, 1H), 1.59 (d, J=11.7 Hz, 1H), 1.07 (br. s., 1H), 0.73-0.65 (m, 1H), 0.64-0.55 (m, 1H), 0.52-0.32 (m, 2H) LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

(c) Hydrido(dimethylphosphinous acid-kP)[Hydrogen bis(dimethyl-phosphinito-kP)]platinum (II) (0.041 g, 0.097 mmol) was added to Compound Q (0.9 g, 1.9 mmol) in 10 mL of 1:1 EtOH/H₂O. The solution was heated at reflux for 2.5 h and concentrated. Purification by MPLC (0-20% MeOH/DCM) followed by preparatory HPLC [0-40% MeCN/H₂O (0.01% TFA)] to yield Compound 118 and Compound 119 as their bis-TFA salts.

Compound 118 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.69 (br. s., 1H), 8.81 (br. s., 1H), 8.01 (t, J=5.3 Hz, 1H), 7.95 (s, 1H), 7.85 (s, 1H), 7.69 (dd, J=8.0, 1.4 Hz, 1H), 7.38 (s, 1H), 7.21 (d, J=8.0 Hz, 1H), 5.96 (s, 1H), 4.14 (d, J=4.7 Hz, 1H), 3.95 (d, J=11.8 Hz, 2H), 3.71-3.24 (m, 6H, overlapped with water), 3.23-3.12 (m, 2H), 3.10-2.92 (m, 7H), 2.86 (d, J=7.2 Hz, 1H), 2.70-2.63 (m, 1H), 2.39-2.14 (m, 2H), 2.00-1.86 (m, 2H), 1.51 (d, J=11.2 Hz, 1H), 1.14-1.02 (m, 1H), 0.75-0.65 (m, 1H), 0.65-0.56 (m, 1H), 0.54-0.38 (m, 2H). LC/MS, m/z=483 [M+H]⁺ (Calc: 482).

Compound 119 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.79 (br. s., 1H), 8.84 (br. s., 1H), 8.33 (t, J=5.3 Hz, 1H), 8.04-7.93 (m, 2H), 7.75 (dd, J=8.0, 1.5 Hz, 1H), 7.39 (s, 1H), 7.27 (d, J=8.1 Hz, 1H), 6.30 (br. s., 1H), 4.17 (d, J=5.2 Hz, 1H), 3.99 (d, J=11.6 Hz, 2H), 3.45-3.26 (m, 8H, overlapped with water), 3.23-3.06 (m, 5H), 3.01 (d, J=12.3 Hz, 1H), 2.91-2.82 (m, 1H), 2.78 (dd, J=12.3, 8.1 Hz, 1H), 2.60-2.54 (m, 1H), 2.44-2.34 (m, 1H), 2.29-2.16 (m, 1H), 2.13-2.04 (m, 1H), 1.97-1.90 (m, 1H), 1.87-1.79 (m, 1H), 1.56 (d, J=12.1 Hz, 1H), 1.13-1.02 (m, 1H), 0.76-0.65 (m, 1H), 0.65-0.55 (m, 1H), 0.52-0.37 (m, 2H). LC/MS, m/z=483 [M+H]⁺ (Calc: 482).

The following compounds were prepared in an analogous fashion from the appropriate phenol:

Compound 120 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.83 (br. s., 1H), 8.17 (t, J=6.0 Hz, 1H), 7.86 (d, J=1.1 Hz, 1H), 7.65 (dd, J=8.0, 1.5 Hz, 1H), 7.35 (d, J=8.0 Hz, 1H), 7.10 (d, J=7.9 Hz, 2H), 6.96 (d, J=8.0 Hz, 2H), 5.99 (s, 1H), 4.17-3.98 (m, 4H), 3.51 (d, J=23.0 Hz, 1H), 3.44-3.31 (m, 2H, overlapped with water), 3.15-2.94 (m, 2H), 2.90-2.79 (m, 1H), 2.63 (d, J=15.6 Hz, 1H), 2.36-2.28 (m, 2H), 2.26 (s, 3H), 2.25-2.09 (m, 1H), 2.03-1.92 (m, 1H), 1.90-1.82 (m, 1H), 1.58 (d, J=12.8 Hz, 1H), 1.08 (br. s., 1H), 0.74-0.65 (m, 1H), 0.64-0.56 (m, 1H), 0.53-0.37 (m, 2H). LC/MS, m/z=456 [M+H]⁺ (Calc: 455).

Compound 121 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.81 (br. s., 1H), 8.57 (t, J=5.9 Hz, 1H), 7.96 (d, J=1.3 Hz, 1H), 7.64 (dd, J=8.0, 1.5 Hz, 1H), 7.31 (d, J=8.1 Hz, 1H), 7.12-7.01 (m, 4H), 6.62 (s, 1H), 4.27-4.10 (m, 3H), 3.53-3.19 (m, 3H, overlapped with water), 2.94 (d, J=13.3 Hz, 1H), 2.78 (dd, J=12.8, 8.4 Hz, 2H), 2.58 (d, J=10.3 Hz, 1H), 2.37-2.23 (m, 2H), 2.21 (s, 3H), 2.13 (d, J=13.2 Hz, 1H), 1.95 (dd, J=13.0, 8.6 Hz, 1H), 1.87-1.67 (m, 2H), 1.52 (d, J=12.2 Hz, 1H), 1.06-0.95 (m, 1H), 0.67-0.58 (m, 1H), 0.58-0.48 (m, 1H), 0.43-0.28 (m, 2H). LC/MS, m/z=456 [M+H]⁺ (Calc: 455).

Compound 117 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.80 (br. s., 1H), 8.11 (t, J=5.9 Hz, 1H), 7.93 (s, 2H), 7.71 (dd, J=8.1, 1.3 Hz, 1H), 7.36 (s, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.08 (d, J=7.8 Hz, 2H), 6.93 (d, J=8.0 Hz, 2H), 5.91 (s, 1H), 4.17-3.97 (m, 3H), 3.38-3.17 (m, 3H, overlapped with water), 3.15-3.05 (m, 1H), 3.00 (d, J=12.8 Hz, 1H), 2.87-2.79 (m, 1H), 2.73-2.66 (m, 1H), 2.41-2.29 (m, 2H), 2.26 (s, 3H), 2.23-2.11 (m, 1H), 1.94 (d, J=8.7 Hz, 2H), 1.52 (d, J=11.7 Hz, 1H), 1.14-1.04 (m, 1H), 0.75-0.65 (m, 1H), 0.64-0.55 (m, 1H), 0.54-0.38 (m, 2H). LC/MS, m/z=474 [M+H]⁺ (Calc: 473).

Compound 116 TFA salt: ¹H NMR (DMSO-d₆) δ: 8.85 (br. s., 1H), 8.69 (t, J=5.9 Hz, 1H), 8.02-7.95 (m, 2H), 7.74 (dd, J=8.1, 1.4 Hz, 1H), 7.37 (s, 1H), 7.26 (d, J=8.1 Hz, 1H), 7.17-7.09 (m, 4H), 6.72 (s, 1H), 4.33-4.15 (m, 3H), 3.53-3.44 (m, 3H), 3.01 (d, J=11.2 Hz, 1H), 2.92-2.80 (m, 2H), 2.73-2.63 (m, 1H), 2.45-2.36 (m, 1H), 2.28 (s, 3H), 2.26-2.16 (m, 1H), 2.04 (dd, J=12.9, 8.5 Hz, 1H), 1.89-1.82 (m, 2H), 1.55 (d, J=11.3 Hz, 1H), 1.12-1.04 (m, 1H), 0.75-0.66 (m, 1H), 0.65-0.56 (m, 1H), 0.51-0.37 (m, 2H). LC/MS, m/z=474 [M+H]⁺ (Calc: 473).

Example 21

The following compounds were prepared by methods analogous to those described above:

Compound 79: ¹H NMR (DMSO-d₆) δ: 9.10 (s., 1H), 8.36-8.33 (d, 1H), 7.86-7.83 (m, 1H), 7.52-7.39 (m, 3H), 6.90 (d, 1H), 6.71 (d, 1H), 6.56 (dd, 1H), 4.58 (s, 1H), 3.96-3.87 (m, 1H), 3.31 (s, 1H), 3.22-3.16 (m, 1H), 2.99 (dd, 1H), 2.77 (d, 1H), 2.57-2.47 (m, 2H), 2.40-2.30 (m, 2H), 2.15-1.99 (m, 3H), 1.88-1.82 (m, 1H), 1.53-1.49 (d, 1H), 1.30 (m, 1H), 0.88-0.82 (m, 1H), 0.48-0.43 (m, 2H), 0.15-0.07 (m, 2H). LC/MS, m/z=419.2 [M+H]⁺ (Calc: 418.2).

Compound 80: δ: 9.46 (s, 1H), 8.85 (d, 1H), 7.43 (d, 2H), 7.02-7.00 (d, 1H), 6.75 (d, 1H), 6.70-6.67 (dd, 1H), 6.56 (dd, 1H), 6.16 (s, 1H), 4.09 (d, 1H), 4.03-4.00 (m, 2H), 3.92 (s, 1H), 3.57 (br, s, 2H), 3.36-3.27 (m, 2H), 3.12-3.00 (m, 2H), 2.85-2.79 (m, 1H), 2.53 (d, 1H), 2.43-2.36 (m, 1H), 2.33-2.28 (m, 2H), 2.26-2.17 (m, 1H), 1.84-1.79 (dd, 1H), 1.51-1.47 (d, 1H), 1.17-1.04 (m, 1H), 0.70-0.55 (m, 2H), 0.51-0.40 (m, 2H). LC/MS, m/z=405.3 [M+H]⁺ (Calc: 404.3).

Compound 81: δ: 9.98 (s, 1H), 9.51-9.36 (d, 1H), 8.87-8.72 (d, 1H), 7.45-7.16 (m, 6H), 7.08-6.97 (2H), 6.86 (s, 1H), 6.66 (m, 1H), 6.17 (s, 1H), 5.67 (br, s, 1H), 4.10-3.71 (m, 6H), 3.38-3.01 (m, 3H), 2.83 (m, 2H), 2.39-2.25 (m, 3H), 2.16-2.07 (m, 1H), 1.86 (m, 1H), 1.67-1.48 (m, 2H), 1.08 (m, 1H), 0.66-0.58 (d, 2H), 0.48-0.41 (d, 2H). LC/MS, m/z=495.3 [M+H]⁺ (Calc: 494.3).

Compound 82: ¹H NMR (DMSO-d₆) δ: 9.27 (s, 1H), 8.78 (s, 1H), 7.74-7.72 (m, 1H), 7.57-7.55 (m, 2H), 7.49-7.45 (m, 1H), 7.39-7.35 (m, 1H), 7.01 (d, 1H), 6.78-6.75 (d, 1H), 6.68-6.65 (dd, 1H), 4.55 (m, 1H), 4.07 (m, 1H), 3.82 (br, 1H), 3.36-3.30 (m, 2H), 3.16-3.10 (m, 1H), 2.99 (dd, 1H), 2.83-2.79 (m, 1H), 2.51-2.50 (m, 2H), 2.47-2.23 (m, 3H), 1.93-1.88 (m, 1H), 1.47-1.44 (m, 1H), 1.09 (m, 1H), 0.72-0.55 (m, 2H), 0.52-0.40 (m, 2H). LC/MS, m/z=419.2 [M+H]⁺ (Calc: 418.2).

Compound 83: ¹H NMR (DMSO-d₆) δ: 9.39 (s, 1H), 8.78 (s, 1H), 8.34 (s, 1H), 7.31-7.29 (d, 2H), 7.19 (m, 2H), 7.03-7.01 (d, 1H), 6.88-6.85 (m, 1H), 6.78 (d, 1H), 6.68-6.66 (dd, 1H), 5.93 (s, 1H), 5.66-5.64 (d, 1H), 4.56-4.48 (m, 1H), 4.02 (m, 1H), 3.36-3.27 (m, 2H), 3.19-3.13 (m, 1H), 3.00-2.97 (d, 1H), 2.85-2.79 (1H), 2.46-2.40 (m, 1H), 2.33-2.26 (m, 2H), 2.15-2.12 (d, 1H), 1.46-1.38 (m, 2H), 1.07 (m, 1H), 0.71-0.56 (m, 2H), 0.50-0.39 (m, 2H). LC/MS, m/z=434.2 [M+H]⁺ (Calc: 433.2).

Compound 86: LC/MS, m/z=488.3 [M+H]⁺ (Calc: 487.3).

Compound 88: LC/MS, m/z=480.3 [M+H]⁺ (Calc: 479.3).

Compound 95: ¹H NMR (DMSO-d₆) δ: 9.09 (s, 1H), 7.77-7.70 (m, 2H), 7.67-7.53 (m, 3H), 7.44 (d, J=5.3 Hz, 1H), 6.86 (d, J=8.4 Hz, 1H), 6.63 (d, J=2.2 Hz, 1H), 6.53 (dd, J=8.3, 2.3 Hz, 1H), 4.31 (d, J=2.0 Hz, 1H), 3.70-3.58 (m, 1H), 3.10 (d, J=4.8 Hz, 1H), 2.96 (d, J=18.0 Hz, 1H), 2.55 (dd, J=18.5, 5.5 Hz, 1H), 2.39-2.16 (m, 3H), 2.12-2.00 (m, 1H), 2.00-1.87 (m, 1H), 1.84-1.72 (m, 1H), 1.65-1.53 (m, 1H), 1.51-1.40 (m, 1H), 1.17 (d, J=12.3 Hz, 1H), 0.85-0.71 (m, 1H), 0.48-0.35 (m, 2H), 0.13-0.00 (m, 2H). LC/MS, m/z=455.1 [M+H]⁺ (Calc: 454.2).

Compound 96 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.36 (br. s., 1H), 8.75 (br. s., 1H), 7.28-7.19 (m, J=8.5, 7.4 Hz, 2H), 7.04-6.91 (m, 3H), 6.87-6.77 (m, 2H), 6.67 (dd, J=8.3, 2.4 Hz, 1H), 6.32 (s, 1H), 4.10 (d, J=4.7 Hz, 1H), 3.75-3.54 (m, 4H overlapped with water), 3.36-3.26 (m, 3H), 3.21-3.06 (m, 6H), 2.99 (d, J=10.0 Hz, 1H), 2.89-2.79 (m, 1H), 2.60 (dd, J=12.6, 8.3 Hz, 1H), 2.41-2.23 (m, 2H), 2.09 (dd, J=12.8, 8.6 Hz, 1H), 1.98-1.92 (m, 2H), 1.49 (d, J=10.8 Hz, 1H), 1.07 (br. s., 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.51-0.44 (m, 1H), 0.44-0.35 (m, 1H). LC/MS, m/z=488 [M+H]⁺ (Calc: 487).

Compound 97 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.30 (s, 1H), 8.99 (br. s., 1H), 8.64 (t, J=5.9 Hz, 1H), 7.13-7.02 (m, 4H), 6.93 (d, J=8.3 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.3, 2.4 Hz, 1H), 6.49 (s, 1H), 4.18 (t, J=5.2 Hz, 2H), 3.74 (d, J=4.9 Hz, 1H), 3.23 (d, J=19.5 Hz, 1H), 3.05 (dd, J=19.8, 5.7 Hz, 1H), 2.92 (d, J=8.7 Hz, 1H), 2.73 (d, J=5.0 Hz, 3H), 2.68-2.58 (m, 1H), 2.54 (dd, J=12.5, 8.4 Hz, 1H), 2.28-2.16 (m, 5H), 1.89 (dd, J=12.5, 8.2 Hz, 1H), 1.82-1.70 (m, 2H), 1.41 (d, J=10.1 Hz, 1H). LC/MS, m/z=407 [M+H]⁺ (Calc: 406).

Compound 98 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.25 (s, 1H), 9.18 (s, 1H), 9.00 (s, 1H), 8.95 (br. s, 1H), 7.93 (t, J=5.8 Hz, 1H), 6.89 (d, J=8.3 Hz, 1H), 6.61 (d, J=2.3 Hz, 1H), 6.58 (d, J=8.3 Hz, 1H), 6.54 (dd, J=8.3, 2.3 Hz, 1H), 6.14 (d, J=2.3 Hz, 1H), 6.07 (dd, J=8.2, 2.4 Hz, 1H), 6.10-6.04 (m, 1H), 5.66 (s, 1H), 3.88 (dd, J=5.6, 2.1 Hz, 2H), 3.65 (d, J=3.9 Hz, 1H), 3.19 (d, J=19.2 Hz, 1H), 3.03 (br. s., 2H), 2.92 (d, J=9.6 Hz, 1H), 2.71 (d, J=5.0 Hz, 3H), 2.48-2.45 (m, 1H), 2.29-2.07 (m, 3H), 1.84 (dd, J=8.7, 4.6 Hz, 2H), 1.36 (d, J=10.1 Hz, 1H). LC/MS, m/z=425 [M+H]⁺ (Calc: 424).

Compound 99 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.26 (br. s., 1H), 9.04 (br. s., 1H), 8.22 (t, J=5.9 Hz, 1H), 7.53 (d, J=8.3 Hz, 1H), 7.39 (d, J=1.9 Hz, 1H), 7.03-6.91 (m, 2H), 6.68 (d, J=2.3 Hz, 1H), 6.61 (dd, J=8.3, 2.4 Hz, 1H), 5.77 (s, 1H), 4.13 (d, J=5.9 Hz, 2H), 3.74 (d, J=3.7 Hz, 1H), 3.27 (d, J=19.0 Hz, 1H), 3.16-2.95 (m, 3H), 2.78 (d, J=5.0 Hz, 3H), 2.56 (dd, J=13.1, 2.8 Hz, 1H), 2.34-2.14 (m, 3H), 2.02-1.85 (m, 2H), 1.44 (d, J=10.0 Hz, 1H). LC/MS, m/z=461 [M+H]⁺ (Calc: 460).

Compound 100 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.25 (br. s., 1H), 9.04 (br. s., 1H), 7.68 (t, J=5.6 Hz, 1H), 7.30-7.22 (m, 2H), 7.22-7.16 (m, 1H), 7.09 (d, J=7.0 Hz, 2H), 6.96 (d, J=8.3 Hz, 1H), 6.67 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.3, 2.4 Hz, 1H), 5.75 (br. s., 2H), 3.73 (d, J=3.7 Hz, 1H), 3.26 (dd, J=17.6, 1.0 Hz, 1H), 3.23-3.06 (m, 3H), 3.06-2.91 (m, 2H), 2.78 (d, J=5.0 Hz, 3H), 2.61-2.55 (m, 2H), 2.34-2.20 (m, 2H), 2.15 (t, J=12.7 Hz, 1H), 1.93-1.80 (m, 2H), 1.42 (d, J=9.4 Hz, 1H). LC/MS, m/z=407 [M+H]⁺ (Calc: 406).

Compound 101 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.31 (br. s., 1H), 8.99 (br. s., 1H), 8.67 (t, J=5.9 Hz, 1H), 7.54 (d, J=8.3 Hz, 1H), 7.43 (d, J=1.9 Hz, 1H), 7.17 (dd, J=8.3, 2.0 Hz, 1H), 6.93 (d, J=8.4 Hz, 1H), 6.71 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.3, 2.3 Hz, 1H), 6.25 (s, 1H), 4.23 (d, J=6.3 Hz, 2H), 3.72 (d, J=4.9 Hz, 1H), 3.22 (d, J=19.5 Hz, 1H), 3.13-3.01 (m, 1H), 2.92 (d, J=8.5 Hz, 1H), 2.73 (d, J=4.9 Hz, 3H), 2.69-2.49 (m, 2H), 2.34-2.13 (m, 2H), 1.92 (dd, J=12.3, 8.3 Hz, 1H), 1.79 (d, J=6.7 Hz, 2H), 1.41 (d, J=9.7 Hz, 1H). LC/MS, m/z=461 [M+H]⁺ (Calc: 460).

Compound 102 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.35 (s, 1H), 9.04 (br. s., 1H), 8.25 (t, J=5.7 Hz, 1H), 6.99 (d, J=8.3 Hz, 1H), 6.77 (d, J=2.4 Hz, 1H), 6.68 (s, 1H), 6.66 (dd, J=8.3, 2.3 Hz, 1H), 3.81 (d, J=3.5 Hz, 1H), 3.31-3.25 (m, 1H, overlapped with water), 3.11 (dd, J=19.3, 5.4 Hz, 1H), 3.02-2.84 (m, 4H), 2.80 (d, J=4.9 Hz, 3H), 2.59 (dd, J=12.4, 8.4 Hz, 1H), 2.38-2.26 (m, 2H), 1.95-1.63 (m, 4H), 1.47 (d, J=9.1 Hz, 1H), 0.84 (d, J=6.7 Hz, 6H). LC/MS, m/z=359 [M+H]⁺ (Calc: 358).

Compound 103 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.30 (br. s., 1H), 9.05 (br. s., 1H), 8.19 (t, J=5.8 Hz, 1H), 7.56 (d, J=2.1 Hz, 1H), 7.35 (dd, J=8.3, 2.1 Hz, 1H), 6.97 (d, J=8.3 Hz, 1H), 6.92 (d, J=8.0 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.63 (dd, J=8.2, 2.3 Hz, 1H), 5.79 (s, 1H), 4.15 (d, J=5.8 Hz, 2H), 3.75 (d, J=4.2 Hz, 1H), 3.28 (d, J=19.4 Hz, 1H), 3.19-2.96 (m, 3H), 2.79 (d, J=5.0 Hz, 3H), 2.60 (dd, J=12.9, 2.6 Hz, 1H), 2.36-2.15 (m, 3H), 2.04-1.89 (m, 2H), 1.45 (d, J=9.8 Hz, 1H). LC/MS, m/z=461 [M+H]⁺ (Calc: 460).

Compound 104 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.20 (br. s., 1H), 8.97 (br. s., 1H), 8.02 (t, J=5.9 Hz, 1H), 7.01 (d, J=7.8 Hz, 2H), 6.93-6.84 (m, 3H), 6.62 (d, J=2.3 Hz, 1H), 6.55 (dd, J=8.3, 2.4 Hz, 1H), 5.68 (br. s., 1H), 4.03 (d, J=5.9 Hz, 2H), 3.67 (d, J=3.9 Hz, 1H), 3.25-3.15 (m, 1H), 3.08-2.89 (m, 3H), 2.71 (d, J=5.0 Hz, 3H), 2.48 (dd, J=13.1, 2.9 Hz, 1H), 2.26-2.10 (m, 7H), 1.86 (d, J=9.1 Hz, 2H), 1.37 (d, J=9.7 Hz, 1H). LC/MS, m/z=407 [M+H]⁺ (Calc: 406).

Compound 105 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.26 (s, 1H), 9.04 (br. s., 1H), 8.13 (t, J=5.9 Hz, 1H), 7.31-7.25 (m, 2H), 7.23-7.16 (m, 1H), 7.05 (d, J=7.2 Hz, 2H), 6.97 (d, J=8.3 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.62 (dd, J=8.3, 2.3 Hz, 1H), 5.75 (s, 1H), 4.15 (d, J=5.8 Hz, 2H), 3.74 (d, J=4.2 Hz, 1H), 3.27 (d, J=19.4 Hz, 1H), 3.15-3.05 (m, 2H), 2.99 (d, J=8.5 Hz, 1H), 2.78 (d, J=5.0 Hz, 3H), 2.57 (dd, J=12.9, 2.9 Hz, 1H), 2.35-2.18 (m, 3H), 1.94 (d, J=8.9 Hz, 2H), 1.44 (d, J=9.6 Hz, 1H). LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

Compound 106 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.37 (s, 1H), 9.07 (br. s., 1H), 8.75 (t, J=5.8 Hz, 1H), 7.36-7.30 (m, 2H), 7.30-7.21 (m, 3H), 7.00 (d, J=8.4 Hz, 1H), 6.77 (d, J=2.3 Hz, 1H), 6.66 (dd, J=8.3, 2.3 Hz, 1H), 6.53 (s, 1H), 4.31 (t, J=5.4 Hz, 2H), 3.81 (d, J=5.1 Hz, 1H), 3.30 (d, J=19.9 Hz, 1H), 3.17-3.08 (m, 1H), 2.99 (d, J=8.4 Hz, 1H), 2.80 (d, J=4.1 Hz, 3H), 2.76-2.66 (m, 1H), 2.62 (dd, J=12.4, 8.5 Hz, 1H), 2.38-2.24 (m, 2H), 1.98 (dd, J=12.6, 8.2 Hz, 1H), 1.91-1.79 (m, 2H), 1.48 (d, J=10.2 Hz, 1H). LC/MS, m/z=393 [M+H]⁺ (Calc: 392).

Compound 107 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.30 (br. s., 1H), 8.98 (br. s., 1H), 8.63 (t, J=5.7 Hz, 1H), 7.56 (d, J=2.1 Hz, 1H), 7.37 (dd, J=8.3, 2.1 Hz, 1H), 7.26 (d, J=8.4 Hz, 1H), 6.93 (d, J=8.4 Hz, 1H), 6.70 (d, J=2.4 Hz, 1H), 6.60 (dd, J=8.3, 2.4 Hz, 1H), 6.22 (s, 1H), 4.36-4.19 (m, 2H), 3.72 (d, J=5.0 Hz, 1H), 3.22 (d, J=19.5 Hz, 1H), 3.17-2.99 (m, 1H), 2.98-2.82 (m, 1H), 2.72 (d, J=5.0 Hz, 3H), 2.69-2.60 (m, 1H), 2.54 (dd, J=12.4, 8.2 Hz, 1H), 2.34-2.13 (m, 3H), 1.93 (dd, J=12.5, 8.6 Hz, 1H), 1.80 (d, J=6.8 Hz, 2H), 1.41 (d, J=9.5 Hz, 1H). LC/MS, m/z=461 [M+H]⁺ (Calc: 460).

Compound 108 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (s, 1H), 8.97 (br. s., 1H), 8.28 (t, J=5.6 Hz, 1H), 7.25-7.19 (m, 2H), 7.17-7.11 (m, 3H), 6.92 (d, J=8.3 Hz, 1H), 6.68 (d, J=2.4 Hz, 1H), 6.59 (dd, J=8.3, 2.3 Hz, 1H), 6.55 (s, 1H), 3.73 (d, J=4.8 Hz, 1H), 3.33-3.15 (m, 3H, overlapped by water), 3.09-3.00 (m, 1H), 2.92 (d, J=7.9 Hz, 1H), 2.72 (d, J=5.0 Hz, 3H), 2.65 (t, J=7.3 Hz, 2H), 2.58-2.45 (m, 2H), 2.29-2.16 (m, 2H), 1.85-1.62 (m, 3H), 1.40 (d, J=9.5 Hz, 1H). LC/MS, m/z=407 [M+H]⁺ (Calc: 406).

Compound 109 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.39 (br. s., 1H), 9.05 (br. s., 1H), 8.55 (d, J=7.3 Hz, 1H), 7.85-7.80 (m, 2H), 7.57-7.51 (m, 1H), 7.51-7.45 (m, 2H), 7.02 (d, J=8.4 Hz, 1H), 6.82 (d, J=2.3 Hz, 1H), 6.70 (dd, J=8.3, 2.3 Hz, 1H), 6.36 (s, 1H), 3.97-3.85 (m, 1H), 3.78 (d, J=4.7 Hz, 1H), 3.34-3.26 (m, 1H), 3.24-3.13 (m, 1H), 3.01 (d, J=8.1 Hz, 1H), 2.81 (d, J=5.0 Hz, 3H), 2.59 (dd, J=12.4, 7.6 Hz, 1H), 2.42-2.30 (m, 2H), 2.28-2.15 (m, 2H), 1.72 (dd, J=14.9, 3.1 Hz, 1H), 1.49 (d, J=9.7 Hz, 1H). LC/MS, m/z=379 [M+H]⁺ (Calc: 378).

Compound 110 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.26 (br. s., 1H), 9.08 (br. s., 1H), 7.72 (d, J=5.9 Hz, 1H), 7.58-7.53 (m, 2H), 7.50-7.44 (m, 1H), 7.41-7.33 (m, 2H), 7.02 (d, J=8.3 Hz, 1H), 6.75 (d, J=2.3 Hz, 1H), 6.66 (dd, J=8.3, 2.3 Hz, 1H), 5.85 (s, 1H), 4.58-4.48 (m, 1H), 3.74 (d, J=3.9 Hz, 1H), 3.32 (d, J=19.2 Hz, 1H), 3.13-2.97 (m, 2H), 2.79 (d, J=5.0 Hz, 3H), 2.46-2.20 (m, 5H), 1.87 (dd, J=13.5, 7.7 Hz, 1H), 1.45 (d, J=9.1 Hz, 1H). LC/MS, m/z=379 [M+H]⁺ (Calc: 378).

Compound 111 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (s, 1H), 9.04 (br. s., 1H), 6.95 (d, J=8.3 Hz, 1H), 6.68 (d, J=2.3 Hz, 1H), 6.58 (dd, J=8.3, 2.3 Hz, 1H), 5.75 (s, 1H), 3.73 (d, J=4.7 Hz, 1H), 3.64-3.30 (m, 9H, overlapped with water), 3.26 (d, J=19.7 Hz, 1H), 3.14-3.07 (m, 1H), 2.99 (d, J=6.5 Hz, 1H), 2.78 (d, J=5.0 Hz, 3H), 2.44 (dd, J=12.8, 3.2 Hz, 1H), 2.26 (d, J=10.9 Hz, 3H), 2.02 (dd, J=12.9, 9.5 Hz, 1H), 1.91-1.83 (m, 1H), 1.44 (d, J=10.1 Hz, 1H). LC/MS, m/z=373 [M+H]⁺ (Calc: 372).

Compound 112 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.73 (br. s., 1H), 9.26 (s, 1H), 9.03 (br. s., 1H), 7.97 (br. s., 1H), 6.95 (d, J=8.3 Hz, 1H), 6.65 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.2, 2.3 Hz, 1H), 5.79 (s, 1H), 3.96 (d, J=12.6 Hz, 2H), 3.74 (d, J=3.9 Hz, 1H), 3.65-3.55 (m, 2H), 3.33-3.17 (m, 3H, overlapped with water), 3.12-2.92 (m, 8H), 2.79 (d, J=5.0 Hz, 3H), 2.57-2.54 (m, 1H), 2.30-2.16 (m, 3H), 1.91 (d, J=8.6 Hz, 2H), 1.44 (d, J=9.1 Hz, 1H). LC/MS, m/z=416 [M+H]⁺ (Calc: 415).

Compound 113 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (s, 1H), 9.17 (br. s., 1H), 9.04 (br. s., 1H), 6.95 (d, J=8.3 Hz, 1H), 6.63 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.2, 2.3 Hz, 1H), 5.80 (s, 1H), 3.74 (br. s., 1H), 3.60-3.22 (m, 4H, overlapped with water), 3.21-3.03 (m, 3H), 3.00 (s, 3H), 2.86 (d, J=4.2 Hz, 1H), 2.79 (d, J=5.0 Hz, 3H), 2.74 (t, J=4.6 Hz, 6H), 2.42 (dd, J=12.8, 3.1 Hz, 1H), 2.36-2.30 (m, 1H), 2.27 (d, J=13.3 Hz, 2H), 2.06 (dd, J=12.7, 9.1 Hz, 1H), 1.86 (dd, J=13.0, 8.1 Hz, 1H), 1.44 (d, J=9.6 Hz, 1H). LC/MS, m/z=388 [M+H]⁺ (Calc: 387).

Compound 114 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.21 (s, 1H), 9.01 (br. s., 1H), 7.60 (t, J=5.6 Hz, 1H), 6.94 (d, J=8.3 Hz, 1H), 6.64 (d, J=2.3 Hz, 1H), 6.58 (dd, J=8.2, 2.3 Hz, 1H), 5.71 (s, 1H), 4.57 (br. s., 1H), 3.72 (d, J=3.7 Hz, 1H), 3.33-3.19 (m, 3H, overlapped with water), 3.13-2.89 (m, 5H), 2.77 (d, J=5.0 Hz, 3H), 2.46 (dd, J=10.3, 2.9 Hz, 1H), 2.24 (s, 3H), 1.88 (d, J=8.7 Hz, 2H), 1.42 (d, J=9.7 Hz, 1H). LC/MS, m/z=347 [M+H]⁺ (Calc: 346).

Compound 115 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.38 (br. s., 1H), 9.05 (br. s., 1H), 8.33 (t, J=5.6 Hz, 1H), 7.00 (d, J=8.3 Hz, 1H), 6.76 (d, J=2.4 Hz, 1H), 6.74 (s, 1H), 6.66 (dd, J=8.3, 2.3 Hz, 1H), 4.73 (br. s., 1H), 3.81 (d, J=4.8 Hz, 1H), 3.41 (t, J=5.9 Hz, 2H), 3.29 (d, J=19.4 Hz, 1H), 3.20-3.06 (m, 3H), 2.99 (d, J=8.3 Hz, 1H), 2.80 (d, J=4.9 Hz, 3H), 2.74-2.64 (m, 1H), 2.58 (dd, J=12.6, 8.6 Hz, 1H), 2.40-2.22 (m, 2H), 1.95-1.71 (m, 3H), 1.47 (d, J=10.0 Hz, 1H). LC/MS, m/z=347 [M+H]⁺ (Calc: 346).

Compound 124 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.17 (s, 1H), 8.96 (s, 1H), 8.65 (br. s., 1H), 8.55 (s, 1H), 8.08 (t, J=5.9 Hz, 1H), 6.88 (d, J=8.3 Hz, 1H), 6.62 (d, J=2.2 Hz, 1H), 6.58-6.51 (m, 2H), 6.48 (t, J=7.7 Hz, 1H), 6.29 (dd, J=7.6, 1.4 Hz, 1H), 5.75 (s, 1H), 4.05-3.93 (m, 3H), 3.27-3.16 (m, 2H, overlapped with water), 3.12-3.00 (m, 2H), 2.91 (d, J=10.6 Hz, 1H), 2.78-2.70 (m, 1H), 2.50-2.45 (m, 1H, overlapped with DMSO), 2.29-2.09 (m, 3H), 1.93-1.80 (m, 2H), 1.37 (d, J=11.1 Hz, 1H), 1.06-0.95 (m, 1H), 0.64-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.46-0.37 (m, 1H), 0.37-0.29 (m, 1H). LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

Compound 125 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.17 (br. s., 1H), 8.65 (br. s., 1H), 8.02 (t, J=5.6 Hz, 1H), 7.24 (d, J=7.8 Hz, 1H), 7.07 (t, J=7.8 Hz, 1H), 6.90-6.85 (m, 2H), 6.62 (d, J=2.3 Hz, 1H), 6.54 (dd, J=8.3, 2.4 Hz, 1H), 5.76 (s, 1H), 4.09 (d, J=5.6 Hz, 2H), 3.99 (d, J=4.2 Hz, 1H), 3.28-3.18 (m, 2H), 3.12-3.02 (m, 2H), 2.91 (d, J=9.9 Hz, 1H), 2.79-2.70 (m, 1H), 2.49 (dd, J=13.0, 2.7 Hz, 1H), 2.32-2.11 (m, 6H), 1.89 (d, J=9.4 Hz, 2H), 1.38 (d, J=10.9 Hz, 1H), 1.07-0.96 (m, 1H), 0.65-0.56 (m, 1H), 0.55-0.48 (m, 1H), 0.45-0.30 (m, 2H). LC/MS, m/z=481 [M+H]⁺ (Calc: 480).

Compound 126 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.16 (s, 1H), 8.65 (br. s., 1H), 8.10 (t, J=5.9 Hz, 1H), 7.18 (d, J=7.9 Hz, 1H), 7.11 (d, J=1.2 Hz, 1H), 6.88 (d, J=8.4 Hz, 1H), 6.84 (dd, J=7.8, 1.4 Hz, 1H), 6.61 (d, J=2.3 Hz, 1H), 6.54 (dd, J=8.3, 2.4 Hz, 1H), 5.75 (s, 1H), 4.09-4.01 (m, 2H), 3.99 (d, J=4.9 Hz, 1H), 3.27-3.17 (m, 2H, overlapped with water), 3.10-2.99 (m, 2H), 2.91 (d, J=10.2 Hz, 1H), 2.78-2.70 (m, 1H), 2.50-2.46 (m, 1H, overlapped with DMSO), 2.30-2.12 (m, 6H), 1.93-1.81 (m, 2H), 1.38 (d, J=11.2 Hz, 1H), 1.06-0.94 (m, 1H), 0.66-0.56 (m, 1H), 0.56-0.47 (m, 1H), 0.45-0.38 (m, 1H), 0.38-0.31 (m, 1H). LC/MS, m/z=481 [M+H]⁺ (Calc: 480).

Compound 127 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.68 (s, 1H), 9.18 (s, 1H), 8.65 (br. s., 1H), 7.96 (t, J=5.7 Hz, 1H), 6.88 (d, J=8.3 Hz, 1H), 6.76 (d, J=8.4 Hz, 1H), 6.70 (d, J=2.4 Hz, 1H), 6.64-6.59 (m, 2H), 6.54 (dd, J=8.3, 2.3 Hz, 1H), 5.75 (s, 1H), 4.02 (d, J=5.7 Hz, 2H), 3.99 (d, J=4.6 Hz, 1H), 3.28-3.18 (m, 2H, overlapped with water), 3.10-3.01 (m, 2H), 2.91 (d, J=9.5 Hz, 1H), 2.79-2.70 (m, 1H), 2.49 (dd, J=13.1, 2.6 Hz, 2H), 2.28-2.11 (m, 3H), 1.88 (d, J=8.8 Hz, 2H), 1.38 (d, J=11.2 Hz, 1H), 1.07-0.96 (m, 1H), 0.65-0.57 (m, 1H), 0.57-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.30 (m, 1H). LC/MS, m/z=483 [M+H]⁺ (Calc: 482).

Compound 128 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.93 (s, 1H), 9.16 (s, 1H), 8.64 (br. s., 1H), 8.02 (t, J=5.9 Hz, 1H), 7.03 (d, J=1.9 Hz, 1H), 6.87 (d, J=8.3 Hz, 1H), 6.83-6.74 (m, 2H), 6.61 (d, J=2.3 Hz, 1H), 6.53 (dd, J=8.3, 2.3 Hz, 1H), 5.74 (s, 1H), 4.01-3.92 (m, 3H), 3.27-3.17 (m, 2H, overlapped with water), 3.10-2.97 (m, 2H), 2.90 (d, J=9.9 Hz, 1H), 2.78-2.69 (m, 1H), 2.49-2.45 (m, 1H), 2.29-2.10 (m, 3H), 1.92-1.79 (m, 2H), 1.37 (d, J=11.3 Hz, 1H), 1.06-0.95 (m, 1H), 0.64-0.57 (m, 1H), 0.56-0.48 (m, 1H), 0.46-0.38 (m, 1H), 0.37-0.30 (m, 1H). LC/MS, m/z=483 [M+H]⁺ (Calc: 482).

Compound 129 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (br. s., 1H), 8.70 (br. s., 1H), 7.67 (t, J=5.6 Hz, 1H), 7.31 (d, J=8.4 Hz, 2H), 7.11 (d, J=8.4 Hz, 2H), 6.95 (d, J=8.3 Hz, 1H), 6.66 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.3, 2.3 Hz, 1H), 5.80 (s, 1H), 4.04 (d, J=4.3 Hz, 1H), 3.36-3.25 (m, 2H), 3.16-3.07 (m, 3H), 3.04-2.94 (m, 2H), 2.85-2.77 (m, 1H), 2.64-2.55 (m, 2H), 2.50-2.45 (m, 1H, overlapped with DMSO), 2.34-2.12 (m, 3H), 1.93-1.83 (m, 2H), 1.43 (d, J=10.2 Hz, 1H), 1.14-1.04 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.52-0.45 (m, 1H), 0.44-0.36 (m, 1H). LC/MS, m/z=481 [M+H]⁺ (Calc: 480).

Compound 130 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (br. s., 1H), 8.71 (br. s., 1H), 7.71 (t, J=5.7 Hz, 1H), 7.56 (d, J=2.1 Hz, 1H), 7.33 (dd, J=8.3, 2.1 Hz, 1H), 7.14 (d, J=8.3 Hz, 1H), 6.95 (d, J=8.4 Hz, 1H), 6.67 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.2, 2.3 Hz, 1H), 5.80 (s, 1H), 4.04 (d, J=4.1 Hz, 1H), 3.35-3.25 (m, 2H), 3.22-3.07 (m, 3H), 3.06-2.93 (m, 2H), 2.85-2.76 (m, 1H), 2.75-2.65 (m, 2H), 2.49-2.46 (m, 1H, overlapped with DMSO), 2.34-2.11 (m, 3H), 1.93-1.81 (m, 2H), 1.43 (d, J=10.3 Hz, 1H), 1.12-1.03 (m, 1H), 0.72-0.63 (m, 1H), 0.62-0.54 (m, 1H), 0.52-0.45 (m, 1H), 0.44-0.37 (m, 1H). LC/MS, m/z=515 [M+H]⁺ (Calc: 514).

Compound 131 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.23 (br. s., 1H), 8.71 (br. s., 1H), 7.68 (t, J=5.6 Hz, 1H), 7.51 (d, J=8.2 Hz, 1H), 7.40 (d, J=1.9 Hz, 1H), 7.07 (dd, J=8.2, 2.0 Hz, 1H), 6.95 (d, J=8.3 Hz, 1H), 6.65 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.2, 2.3 Hz, 1H), 5.80 (s, 1H), 4.04 (d, J=4.2 Hz, 1H), 3.35-3.24 (m, 2H), 3.20-3.09 (m, 3H), 3.09-2.93 (m, 3H), 2.85-2.76 (m, 1H), 2.65-2.55 (m, 2H), 2.50-2.44 (m, 1H, overlapped with DMSO), 2.34-2.10 (m, 4H), 1.92-1.81 (m, 2H), 1.43 (d, J=10.4 Hz, 1H), 1.13-1.04 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.52-0.46 (m, 1H), 0.45-0.37 (m, 1H). LC/MS, m/z=515 [M+H]⁺ (Calc: 514).

Compound 132 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.52 (br. s., 1H), 9.17 (s, 1H), 8.69 (br. s., 1H), 6.87 (d, J=7.8 Hz, 1H), 6.63 (d, J=37.2 Hz, 1H), 6.52 (dd, J=8.3, 2.4 Hz, 1H), 5.83 (br. s., 1H), 4.34-4.20 (m, 1H), 4.08-3.94 (m, 2H), 3.26-3.18 (m, 3H), 3.10-3.02 (m, 1H), 2.95-2.88 (m, 1H), 2.82-2.70 (m, 3H), 2.54-2.46 (m, 1H), 2.37-2.11 (m, 4H), 2.07-1.96 (m, 1H), 1.95-1.79 (m, 1H), 1.38 (d, J=11.7 Hz, 1H), 1.24-1.15 (m, 6H), 1.07-0.96 (m, 1H), 0.65-0.58 (m, 1H), 0.57-0.48 (m, 1H), 0.46-0.39 (m, 1H), 0.38-0.30 (m, 1H). LC/MS, m/z=454 [M+H]⁺ (Calc: 453).

Compound 133 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.38 (s, 1H), 9.15 (br. s., 1H), 8.73 (br. s., 1H), 8.36 (t, J=5.6 Hz, 1H), 6.99 (d, J=8.4 Hz, 1H), 6.77 (d, J=2.3 Hz, 1H), 6.67 (dd, J=8.3, 2.4 Hz, 1H), 6.24 (s, 1H), 4.09 (d, J=4.9 Hz, 1H), 3.36-3.27 (m, 2H, overlapped with water), 3.20-3.06 (m, 4H), 3.01-2.79 (m, 4H), 2.63-2.53 (m, 3H), 2.39-2.23 (m, 2H), 2.06-1.98 (m, 1H), 1.97-1.77 (m, 4H), 1.75-1.55 (m, 3H), 1.49 (d, J=10.3 Hz, 1H), 1.44-1.32 (m, 1H), 1.11-1.01 (m, 1H), 0.73-0.64 (m, 1H), 0.64-0.54 (m, 1H), 0.50-0.35 (m, 2H). LC/MS, m/z=454 [M+H]⁺ (Calc: 453).

Compound 134 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 10.03 (br. s., 1H), 9.29 (br. s., 1H), 8.73 (br. s., 1H), 8.05-7.96 (m, 1H), 6.94 (d, J=8.3 Hz, 1H), 6.65 (d, J=2.2 Hz, 1H), 6.60 (dd, J=8.2, 2.3 Hz, 1H), 5.90 (br. s., 1H), 4.07 (d, J=4.7 Hz, 1H), 3.82-3.71 (m, 3H), 3.49-3.36 (m, 3H), 3.35-3.24 (m, 4H), 3.15-2.90 (m, 5H), 2.87-2.79 (m, 1H), 2.66-2.54 (m, 2H), 2.35-2.17 (m, 3H), 1.99-1.87 (m, 2H), 1.44 (d, J=10.3 Hz, 1H), 1.12 (d, J=6.1 Hz, 6H), 1.10-1.03 (m, 1H), 0.73-0.64 (m, 1H), 0.63-0.54 (m, 1H), 0.53-0.45 (m, 1H), 0.44-0.36 (m, 1H). LC/MS, m/z=484 [M+H]⁺ (Calc: 483).

Compound 135 bis-TFA salt: ¹H NMR (DMSO-d₆) δ: 9.96 (br. s., 1H), 9.38 (s, 1H), 8.74 (br. s., 1H), 8.40-8.32 (m, 1H), 6.99 (d, J=8.4 Hz, 1H), 6.77 (d, J=2.3 Hz, 1H), 6.67 (dd, J=8.3, 2.3 Hz, 1H), 6.24 (br. s., 1H), 4.09 (d, J=5.3 Hz, 1H), 3.86-3.75 (m, 2H), 3.37-3.26 (m, 5H), 3.22-3.07 (m, 3H), 2.99 (d, J=8.9 Hz, 1H), 2.88-2.79 (m, 1H), 2.58 (br. s., 4H), 2.40-2.23 (m, 2H), 2.05-1.80 (m, 3H), 1.49 (d, J=9.9 Hz, 1H), 1.13 (d, J=5.6 Hz, 6H), 1.10-1.01 (m, 1H), 0.72-0.65 (m, 1H), 0.64-0.55 (m, 1H), 0.53-0.44 (m, 1H), 0.44-0.37 (m, 1H). LC/MS, m/z=484 [M+H]⁺ (Calc: 483).

Compound 136 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.21 (s, 1H), 8.65 (br. s., 1H), 8.12 (t, J=5.9 Hz, 1H), 7.29 (dd, J=10.0, 2.1 Hz, 1H), 7.14 (dd, J=8.3, 1.9 Hz, 1H), 6.93-6.86 (m, 2H), 6.62 (d, J=2.3 Hz, 1H), 6.55 (dd, J=8.2, 2.3 Hz, 1H), 5.77 (s, 1H), 4.07 (d, J=5.8 Hz, 2H), 3.99 (d, J=4.1 Hz, 1H), 3.28-3.18 (m, 2H, overlapped with water), 3.11-3.01 (m, 2H), 2.91 (d, J=9.5 Hz, 1H), 2.79-2.70 (m, 1H), 2.50 (dd, J=13.0, 2.5 Hz, 1H), 2.29-2.12 (m, 3H), 1.88 (d, J=8.8 Hz, 2H), 1.38 (d, J=10.7 Hz, 1H), 1.06-0.95 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.30 (m, 1H) LC/MS, m/z=485 [M+H]⁺ (Calc: 484).

Compound 137 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (br. s., 1H), 8.72-8.62 (m, 2H), 7.24 (d, J=7.9 Hz, 1H), 7.21 (d, J=1.3 Hz, 1H), 7.05 (dd, J=7.7, 1.5 Hz, 1H), 6.92 (d, J=8.4 Hz, 1H), 6.71 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.3, 2.4 Hz, 1H), 6.43 (s, 1H), 4.20 (d, J=5.9 Hz, 2H), 4.04 (d, J=5.0 Hz, 1H), 3.30-3.19 (m, 2H), 3.14-3.04 (m, 1H), 2.92 (d, J=9.8 Hz, 1H), 2.81-2.71 (m, 1H), 2.68-2.51 (m, 2H), 2.34-2.17 (m, 5H), 1.93 (dd, J=12.4, 8.2 Hz, 1H), 1.80 (d, J=6.5 Hz, 2H), 1.42 (d, J=10.9 Hz, 1H), 1.05-0.94 (m, 1H), 0.66-0.57 (m, 1H), 0.57-0.48 (m, 1H), 0.44-0.28 (m, 2H). LC/MS, m/z=481 [M+H]⁺ (Calc: 480).

Compound 138 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.28 (s, 1H), 8.68 (br. s., 1H), 8.55 (t, J=5.5 Hz, 1H), 7.30 (dd, J=7.0, 2.2 Hz, 1H), 7.16-7.09 (m, 2H), 6.91 (d, J=8.4 Hz, 1H), 6.71 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.3, 2.3 Hz, 1H), 6.44 (s, 1H), 4.32-4.21 (m, 2H), 4.04 (d, J=5.4 Hz, 1H), 3.29-3.20 (m, 2H, overlapped with water), 3.14-3.03 (m, 1H), 2.92 (d, J=10.0 Hz, 1H), 2.82-2.71 (m, 1H), 2.68-2.51 (m, 2H, overlapped with DMSO), 2.34-2.19 (m, 5H), 1.93 (dd, J=12.5, 8.2 Hz, 1H), 1.80 (d, J=6.6 Hz, 2H), 1.42 (d, J=11.3 Hz, 1H), 1.06-0.94 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.48 (m, 1H), 0.44-0.29 (m, 2H). LC/MS, m/z=481 [M+H]⁺ (Calc: 480).

Compound 139 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.15 (s, 1H), 9.05 (s, 1H), 8.69-8.61 (m, 1H), 8.02 (t, J=5.9 Hz, 1H), 6.87 (d, J=8.3 Hz, 1H), 6.60 (d, J=2.1 Hz, 1H), 6.56-6.47 (m, 1H), 6.00-5.94 (m, 2H), 5.73 (s, 1H), 3.98 (d, J=3.5 Hz, 1H), 3.95-3.82 (m, 2H), 3.24-3.17 (m, 2H, overlapped with water), 3.09-3.00 (m, 2H), 2.91 (d, J=9.8 Hz, 1H), 2.81-2.71 (m, 1H), 2.51-2.45 (m, 2H), 2.30-2.09 (m, 3H), 1.94-1.78 (m, 2H), 1.37 (d, J=11.4 Hz, 1H), 1.05-0.96 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.45-0.38 (m, 1H), 0.38-0.28 (m, 1H). LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

Compound 140 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.17 (s, 1H), 8.70 (s, 1H), 8.64 (br. s., 2H), 7.95 (t, J=5.9 Hz, 1H), 6.87 (d, J=8.3 Hz, 1H), 6.61 (d, J=2.3 Hz, 1H), 6.56 (d, J=8.0 Hz, 1H), 6.53 (dd, J=8.3, 2.3 Hz, 1H), 6.48 (d, J=1.9 Hz, 1H), 6.28 (dd, J=8.0, 1.9 Hz, 1H), 5.73 (s, 1H), 3.98 (d, J=4.3 Hz, 1H), 3.94-3.83 (m, 2H), 3.27-3.17 (m, 2H, overlapped with water), 3.10-2.97 (m, 2H), 2.90 (d, J=9.9 Hz, 1H), 2.79-2.69 (m, 1H), 2.50-2.45 (m, 1H, overlapped with DMSO), 2.28-2.09 (m, 3H), 1.85 (d, J=8.9 Hz, 2H), 1.37 (d, J=11.2 Hz, 1H), 1.06-0.95 (m, 1H), 0.65-0.57 (m, 1H), 0.55-0.46 (m, 1H), 0.45-0.37 (m, 1H), 0.36-0.30 (m, 1H). LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

Compound 141 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.28 (s, 1H), 9.18 (s, 1H), 8.69 (br. s., 1H), 8.55 (t, J=5.6 Hz, 1H), 8.50 (s, 1H), 6.91 (d, J=8.4 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.67-6.56 (m, 3H), 6.55-6.45 (m, 2H), 4.22-4.09 (m, 2H), 4.04 (d, J=3.9 Hz, 1H), 3.27-3.19 (m, 2H, overlapped with water), 3.13-3.04 (m, 1H), 2.92 (d, J=10.7 Hz, 1H), 2.81-2.72 (m, 1H), 2.71-2.64 (m, 1H), 2.59-2.51 (m, 1H), 2.34-2.15 (m, 2H), 1.91 (dd, J=12.8, 8.3 Hz, 1H), 1.82-1.72 (m, 2H), 1.42 (d, J=10.9 Hz, 1H), 1.05-0.94 (m, 1H), 0.66-0.58 (m, 1H), 0.57-0.48 (m, 1H), 0.44-0.36 (m, 1H), 0.35-0.28 (m, 1H). LC/MS, m/z=465 [M+H]⁺ (Calc: 464).

Compound 142 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.22 (s, 1H), 9.16 (s, 1H), 8.64 (br. s., 1H), 8.05 (t, J=5.9 Hz, 1H), 7.00 (t, J=7.8 Hz, 1H), 6.88 (d, J=8.3 Hz, 1H), 6.61 (d, J=2.3 Hz, 1H), 6.56-6.51 (m, 2H), 6.49 (s, 1H), 6.42 (d, J=7.6 Hz, 1H), 5.74 (s, 1H), 4.05-3.93 (m, 3H), 3.28-3.17 (m, 2H, overlapped with water), 3.10-2.99 (m, 2H), 2.91 (d, J=10.8 Hz, 1H), 2.78-2.70 (m, 1H), 2.50-2.46 (m, 1H, overlapped with DMSO), 2.29-2.10 (m, 3H), 1.93-1.82 (m, 2H), 1.38 (d, J=11.0 Hz, 1H), 1.01 (br. s., 1H), 0.66-0.56 (m, 1H), 0.56-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.28 (m, 1H). LC/MS, m/z=449 [M+H]⁺ (Calc: 448).

Compound 143 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (s, 1H), 8.73-8.62 (m, 2H), 7.05 (tt, J=9.4, 2.3 Hz, 1H), 6.94-6.85 (m, 3H), 6.72 (d, J=2.3 Hz, 1H), 6.60 (dd, J=8.3, 2.4 Hz, 1H), 6.29 (s, 1H), 4.26 (d, J=5.9 Hz, 2H), 4.04 (d, J=4.8 Hz, 1H), 3.28-3.20 (m, 2H, overlapped with water), 3.15-3.06 (m, 1H), 2.92 (d, J=9.4 Hz, 1H), 2.81-2.72 (m, 1H), 2.67-2.51 (m, 2H), 2.33-2.18 (m, 2H), 1.96 (dd, J=12.2, 8.4 Hz, 1H), 1.85-1.80 (m, 2H), 1.42 (d, J=11.0 Hz, 1H), 1.04-0.95 (m, 1H), 0.65-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.44-0.36 (m, 1H), 0.36-0.28 (m, 1H). LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

Compound 144 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (s, 1H), 8.72-8.62 (m, 2H), 7.33 (dt, J=10.8, 8.5 Hz, 1H), 7.22 (ddd, J=11.7, 7.9, 2.0 Hz, 1H), 7.06-7.00 (m, 1H), 6.92 (d, J=8.4 Hz, 1H), 6.71 (d, J=2.4 Hz, 1H), 6.59 (dd, J=8.3, 2.4 Hz, 1H), 6.36 (s, 1H), 4.22 (d, J=5.8 Hz, 2H), 4.03 (d, J=4.7 Hz, 1H), 3.29-3.19 (m, 2H, overlapped with water), 3.14-3.04 (m, 1H), 2.92 (d, J=9.8 Hz, 1H), 2.81-2.72 (m, 1H), 2.67-2.50 (m, 2H), 2.33-2.16 (m, 2H), 1.95 (dd, J=12.2, 8.3 Hz, 1H), 1.85-1.79 (m, 2H), 1.42 (d, J=11.2 Hz, 1H), 1.05-0.95 (m, 1H), 0.66-0.57 (m, 1H), 0.57-0.47 (m, 1H), 0.43-0.29 (m, 2H). LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

Compound 145 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.17 (br. s., 1H), 8.65 (br. s., 1H), 8.13 (t, J=6.0 Hz, 1H), 7.25 (dt, J=10.8, 8.5 Hz, 1H), 7.07 (ddd, J=11.6, 8.0, 2.0 Hz, 1H), 6.88 (d, J=8.3 Hz, 1H), 6.81-6.76 (m, 1H), 6.61 (d, J=2.3 Hz, 1H), 6.54 (dd, J=8.3, 2.3 Hz, 1H), 5.77 (s, 1H), 4.05 (d, J=5.8 Hz, 2H), 3.99 (d, J=4.2 Hz, 1H), 3.28-3.18 (m, 2H), 3.10-3.00 (m, 2H), 2.91 (d, J=9.6 Hz, 1H), 2.78-2.71 (m, 1H), 2.50 (dd, J=13.1, 2.5 Hz, 1H), 2.28-2.11 (m, 3H), 1.92-1.84 (m, 2H), 1.38 (d, J=10.7 Hz, 1H), 1.07-0.96 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.48 (m, 1H), 0.46-0.38 (m, 1H), 0.37-0.30 (m, 1H). LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

Compound 146 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.29 (s, 1H), 8.68 (br. s., 1H), 8.61 (t, J=5.7 Hz, 1H), 7.32-7.24 (m, 1H), 7.20-7.14 (m, 1H), 7.01 (td, J=8.6, 2.2 Hz, 1H), 6.91 (d, J=8.3 Hz, 1H), 6.70 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.3, 2.3 Hz, 1H), 6.37 (s, 1H), 4.29-4.18 (m, 2H), 4.03 (d, J=5.0 Hz, 1H), 3.28-3.18 (m, 2H, overlapped with water), 3.13-3.04 (m, 1H), 2.91 (d, J=9.7 Hz, 1H), 2.81-2.72 (m, 1H), 2.66-2.57 (m, 1H), 2.57-2.49 (m, 1H), 2.33-2.16 (m, 2H), 1.97-1.88 (m, 1H), 1.79 (d, J=6.6 Hz, 2H), 1.41 (d, J=10.7 Hz, 1H), 1.05-0.94 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.44-0.28 (m, 2H). LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

Compound 147 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.20 (br. s., 1H), 8.65 (br. s., 1H), 8.09 (t, J=5.8 Hz, 1H), 7.11 (t, J=9.9 Hz, 1H), 6.96-6.90 (m, 2H), 6.88 (d, J=8.4 Hz, 1H), 6.62 (d, J=2.3 Hz, 1H), 6.55 (dd, J=8.3, 2.3 Hz, 1H), 5.77 (s, 1H), 4.07 (d, J=5.7 Hz, 2H), 3.99 (d, J=4.3 Hz, 1H), 3.28-3.18 (m, 2H), 3.11-3.00 (m, 2H), 2.91 (d, J=9.6 Hz, 1H), 2.78-2.70 (m, 1H), 2.49 (dd, J=13.0, 2.6 Hz, 1H), 2.29-2.11 (m, 3H), 1.88 (d, J=9.0 Hz, 2H), 1.38 (d, J=11.1 Hz, 1H), 1.06-0.93 (m, 1H), 0.66-0.56 (m, 1H), 0.56-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.31 (m, 1H). LC/MS, m/z=469 [M+H]⁺ (Calc: 468).

Compound 148 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.18 (s, 1H), 8.66 (br. s., 1H), 8.10 (t, J=5.8 Hz, 1H), 7.37-7.29 (m, 1H), 7.29-7.13 (m, 2H), 6.93-6.85 (m, 2H), 6.64 (d, J=2.3 Hz, 1H), 6.55 (dd, J=8.3, 2.3 Hz, 1H), 5.78 (s, 1H), 4.13 (d, J=5.8 Hz, 2H), 4.00 (d, J=4.2 Hz, 1H), 3.29-3.18 (m, 2H), 3.15-3.02 (m, 2H), 2.92 (d, J=10.1 Hz, 1H), 2.80-2.69 (m, 1H), 2.54 (dd, J=13.0, 2.4 Hz, 1H), 2.30-2.12 (m, 3H), 1.91 (d, J=9.6 Hz, 2H), 1.39 (d, J=11.2 Hz, 1H), 1.07-0.96 (m, 1H), 0.65-0.57 (m, 1H), 0.57-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.28 (m, 1H). LC/MS, m/z=514 [M+H]⁺ (Calc: 514).

Compound 149 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.37 (s, 1H), 9.17 (s, 1H), 8.65 (br. s., 1H), 8.00 (t, J=5.9 Hz, 1H), 6.98-6.92 (m, 1H), 6.88 (d, J=8.4 Hz, 1H), 6.75 (d, J=6.3 Hz, 1H), 6.69-6.61 (m, 3H), 6.54 (dd, J=8.3, 2.3 Hz, 1H), 5.75 (s, 1H), 4.07-3.94 (m, 3H), 3.28-3.18 (m, 2H, overlapped with water), 3.14-3.02 (m, 2H), 2.91 (d, J=10.4 Hz, 1H), 2.81-2.69 (m, 1H), 2.50 (dd, J=13.1, 2.8 Hz, 1H, overlapped with DMSO), 2.29-2.12 (m, 3H), 1.94-1.81 (m, 2H), 1.38 (d, J=11.0 Hz, 1H), 1.08-0.91 (m, 1H), 0.66-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.46-0.38 (m, 1H), 0.38-0.30 (m, 1H). LC/MS, m/z=449 [M+H]⁺ (Calc: 448).

Compound 150 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.19 (s, 1H), 8.72 (br. s., 1H), 8.25 (t, J=5.9 Hz, 1H), 7.46 (t, J=1.9 Hz, 1H), 7.18 (d, J=1.8 Hz, 2H), 6.94 (d, J=8.3 Hz, 1H), 6.66 (d, J=2.3 Hz, 1H), 6.59 (dd, J=8.3, 2.4 Hz, 1H), 5.83 (s, 1H), 4.22-4.04 (m, 3H), 3.35-3.24 (m, 2H), 3.19-3.08 (m, 2H), 2.98 (d, J=10.3 Hz, 1H), 2.86-2.78 (m, 1H), 2.58-2.53 (m, 1H), 2.37-2.19 (m, 3H), 2.03-1.86 (m, 2H), 1.45 (d, J=11.5 Hz, 1H), 1.14-1.04 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.55 (m, 1H), 0.53-0.38 (m, 2H). LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

Compound 151 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.19 (s, 1H), 8.73 (br. s., 1H), 8.24 (t, J=5.7 Hz, 1H), 7.46 (d, J=8.5 Hz, 1H), 7.35 (dd, J=8.4, 2.6 Hz, 1H), 7.19 (d, J=2.5 Hz, 1H), 6.94 (d, J=8.4 Hz, 1H), 6.66 (d, J=2.3 Hz, 1H), 6.58 (dd, J=8.3, 2.4 Hz, 1H), 5.84 (s, 1H), 4.26-4.12 (m, 2H), 4.07 (d, J=4.3 Hz, 1H), 3.35-3.24 (m, 2H), 3.24-3.08 (m, 2H), 2.98 (d, J=10.7 Hz, 1H), 2.87-2.77 (m, 1H), 2.55 (dd, J=12.9, 3.1 Hz, 2H), 2.37-2.17 (m, 3H), 2.05-1.87 (m, 2H), 1.45 (d, J=11.5 Hz, 1H), 1.13-1.04 (m, 1H), 0.72-0.64 (m, 1H), 0.63-0.56 (m, 1H), 0.53-0.46 (m, 1H), 0.45-0.36 (m, 1H). LC/MS, m/z=501 [M+H]⁺ (Calc: 500).

Compound 152 TFA salt: ¹H NMR (DMSO-d₆) δ: 9.16 (s, 1H), 8.65 (br. s., 1H), 8.14 (t, J=6.0 Hz, 1H), 7.31-7.18 (m, 2H), 7.13 (s, 1H), 6.93 (d, J=7.4 Hz, 1H), 6.88 (d, J=8.3 Hz, 1H), 6.62 (d, J=2.3 Hz, 1H), 6.54 (dd, J=8.3, 2.4 Hz, 1H), 5.76 (s, 1H), 4.11-4.04 (m, 2H), 3.99 (d, J=4.6 Hz, 1H), 3.29-3.15 (m, 2H, overlapped with water), 3.11-3.00 (m, 2H), 2.91 (d, J=10.1 Hz, 1H), 2.79-2.69 (m, 1H), 2.49 (dd, J=12.9, 2.9 Hz, 1H), 2.29-2.11 (m, 3H), 1.96-1.81 (m, 2H), 1.38 (d, J=11.3 Hz, 1H), 1.06-0.93 (m, 1H), 0.64-0.57 (m, 1H), 0.56-0.47 (m, 1H), 0.45-0.38 (m, 1H), 0.37-0.28 (m, 1H). LC/MS, m/z=467 [M+H]⁺ (Calc: 466).

Example 22

The following Tables provide results on the efficacy of binding and activity response of exemplified Compounds of the Invention at the ORL-1, μ-, δ-, and κ-opioid receptors.

In TABLE 3, binding affinity of certain Compounds of the Invention to the ORL-1, μ-, δ-, and κ-opioid receptors was determined as described above.

In TABLE 4, activity response of certain Compounds of the Invention to the μ- and κ-opioid receptors was determined as described above for functional assays using HEK-293 or CHO cells.

TABLE 3 Binding Affinity of Certain Compounds of the Invention K_(i) (nM) Compd. Opioid Receptor No. ORL-1 μ κ δ 6 10273 ± 2145 19.7 ± 4.68 0.098 ± 0.032 7714 ± 1278 7 23.6 ± 5.56  0.13 ± 0.018 11  2982 ± 466.1 182.0 ± 29.2  12  0.53 ± 0.076  0.30 ± 0.056 16 55.6 ± 11.0 17.3 ± 6.19 31 >20 μM 10.2 ± 3.58 0.84 ± 0.26 33  1.39 ± 0.082 0.75 ± 0.13 34 0.083 ± 0.014 38  0.16 ± 0.044 42  0.40 ± 0.024 0.37 ± 0.13 93  0.64 ± 0.071 108 9.29 ± 2.14

TABLE 4 Activity Response of Certain Compounds of the Invention Opioid Receptor μ κ δ Compd EC₅₀ E_(max) EC₅₀ E_(max) EC₅₀ E_(max) No. (nM) (%) (nM) (%) (nM) (%) 6 >20 μM 0.00 0.86 ± 0.16 48.6 ± 0.98 7 >20 μM 1.00 ± 0.00 0.80 ± 0.26 57.5 ± 1.66 12 >20 μM 1.00 ± 0.00 2.95 ± 1.06 82.7 ± 1.20 15 >20 μM 7.00 16 >20 μM 1.00 ± 0.00 12.4 ± 3.66 38.0 ± 2.04 22 >20 μM 1.00 ± 0.00 25.7 ± 4.99 77.0 ± 1.00 25 616.2 ± 40.9  12.0 ± 2.08 28 >20 μM 7.50 30 >20 μM 1.00 ± 0.00 2.49 ± 0.090 50.7 ± 0.33 31 >20 μM 1.00 ± 0.00 6.95 ± 0.97 35.3 ± 2.19 75.5 ± 13.6  32.0 ± 3.49 33 >20 μM 1.00 7.03 ± 0.82 43.3 ± 0.33 34 3.75 ± 0.66 40.0 ± 1.53 0.094 ± 0.011 101.7 ± 2.73  36 >20 μM 1.00 ± 0.00 2.24 ± 0.46 94.7 ± 3.71 37 >20 μM 1.00 1.74 ± 0.39 32.5 ± 2.10 38 0.62 ± 0.12 56.5 ± 2.60 0.43 ± 0.048 96.3 ± 3.18 1.56 ± 0.055 50.1 ± 1.25 39 37.5 ± 12.6 15.0 ± 1.73 40 >20 μM 1.00 41 >20 μM 1.00 1.74 ± 0.47 35.8 ± 3.07 42 >20 μM 1.00 0.32 ± 0.059 98.3 ± 1.33 43 8.35 ± 1.80 11.0 ± 0.82 45  0.38 ± 0.044 43.3 ± 4.84 46 >20 μM 0.00 0.20 ± 0.072 32.0 ± 5.00 47  2.76 ± 0.082 86.0 ± 3.46 0.030 ± 0.01 88.8 ± 10.0 48 >20 μM −1.00 ± 0.00  49 6.17 ± 1.98 10.5 ± 0.29 51  0.49 ± 0.040 43.0 ± 3.06 64 >20 μM 3.50 77 580.3 ± 131.4 19.6 ± 5.65 78 308.0 ± 93.0  27.4 ± 1.03 79 >20 μM −1.00  80 >20 μM 1.00 81 >20 μM 19.0 ± 1.53 82 >20 μM 1.00 ± 0.00 83 >20 μM 0.00 84 71.7 ± 23.7 19.7 ± 1.67 85 >20 μM −1.00  86 28.8 ± 10.5 12.8 ± 0.63 87 >20 μM 1.00 88 >20 μM 0.00 89 >20 μM 4.50 90 >20 μM 8.14 ± 1.30 91 >20 μM −1.00 ± 0.00  92 >20 μM −1.00  93 3.95 ± 0.65 28.8 ± 4.91 1.52 ± 0.17 81.5 ± 1.27 94 >20 μM 10.6 ± 3.41

The in vitro test results of Tables 3 and 4 show that representative Compounds of the Invention generally have high binding affinity for opioid receptors, and that these compounds activate these receptors as partial to full agonists. Compounds of the Invention are therefore expected to be useful to treat Conditions, particularly pain, that are responsive to the activation of one or more opioid receptors.

Example 23

The following TABLE 5 provides results on the activity response of certain Compounds of the Invention at the μ- and κ-opioid receptors determined as described above using U-2 OS cells.

TABLE 5 Activity Response of Certain Compounds of the Invention Opioid Receptor Compd. μ κ No. EC₅₀ (nM) E_(max) (%) EC₅₀ (nM) E_(max) (%) 6 16.0 ± 0.91 57.0 ± 2.08 0.87 ± 0.16  98.0 ± 3.21 7 8.94 ± 1.31 56.3 ± 2.60 1.16 ± 0.16 101.3 ± 3.67 11 1358 ± 49.8  103.3 ± 1.67 12 1.22 ± 0.27 55.6 ± 3.83 1.70 ± 0.26 108.0 ± 3.51 14 125.8 ± 37.7  26.7 ± 2.40 147.3 ± 11.6  100.0 ± 2.52 15 342.6 ± 110.4 31.6 ± 3.21 2896 ± 82.3   79.7 ± 5.81 16 4.75 ± 0.62 31.8 ± 3.07 13.2 ± 1.86  90.7 ± 5.81 19  2965 ± 863.8 29.3 ± 5.93  4721 ± 150.1  97.7 ± 0.67 20 1233 ± 83.5  43.3 ± 2.40  4398 ± 357.1  87.7 ± 4.33 21 >20 μM 8.67 ± 1.76  5594 ± 738.2  90.0 ± 2.08 22 110.6 ± 14.1  20.7 ± 2.40 25.4 ± 2.09 100.7 ± 1.76 25 227.5 ± 25.4  54.3 ± 4.10 234.1 ± 28.5  107.0 ± 4.16 26  1131 ± 116.8 63.0 ± 5.77 828.9 ± 100.4 106.3 ± 2.03 28 325.7 ± 77.9  35.7 ± 5.49 178.6 ± 16.4  106.7 ± 0.88 29 7.78 ± 1.63 22.0 ± 1.73  0.62 ± 0.040 106.7 ± 3.18 30 1.62 ± 0.41 32.0 ± 1.15  1.26 ± 0.050 100.3 ± 1.20 31 2.35 ± 0.32 49.0 ± 3.76  2.62 ± 0.024  87.5 ± 5.04 33 3.84 ± 0.21 45.7 ± 0.67 3.18 ± 0.46 102.7 ± 3.48 34  0.25 ± 0.027 94.0 ± 0.58 0.020 ± 0.00   93.3 ± 3.18 35 19.7 ± 5.27 29.7 ± 0.33 7.75 ± 0.41 110.7 ± 2.96 36 1.96 ± 0.27 47.7 ± 3.18  0.27 ± 0.019 108.3 ± 4.10 37 8.48 ± 2.28 30.7 ± 2.67 0.97 ± 0.11 105.3 ± 0.88 38  0.25 ± 0.024 77.7 ± 2.40 0.065 ± 0.01  105.3 ± 3.18 39 11.1 ± 0.28 42.0 ± 1.00  1.85 ± 0.097  91.7 ± 1.86 40  1.45 ± 0.068 29.3 ± 1.33  0.73 ± 0.070  97.7 ± 1.86 41 4.19 ± 1.02 25.3 ± 1.33  1.02 ± 0.080  86.7 ± 6.74 42 1.33 ± 0.12 35.0 ± 1.15  1.27 ± 0.076  87.7 ± 4.63 43 4.55 ± 0.14 41.3 ± 2.03  0.67 ± 0.020 101.7 ± 5.90 44 3.75 ± 0.35 28.3 ± 1.86  0.26 ± 0.033  94.7 ± 7.13 45  0.14 ± 0.018 87.0 ± 0.58 0.018 ± 0.00  100.0 ± 4.62 46  0.91 ± 0.050 38.0 ± 1.73 0.11 ± 0.01 106.3 ± 4.26 47 0.050 ± 0.01  98.8 ± 1.49 0.015 ± 0.00   95.0 ± 5.29 48 3.12 ± 0.27 40.0 ± 1.53 1.46 ± 0.29 106.3 ± 2.33 49 3.73 ± 0.23 42.3 ± 1.45  0.82 ± 0.099 108.3 ± 4.70 50 6.90 ± 0.54 23.3 ± 1.86 1.95 ± 0.33 107.7 ± 0.88 51  0.13 ± 0.015 87.7 ± 1.20 0.047 ± 0.00  102.0 ± 2.31 56 10.4 ± 2.03 16.0 ± 1.53 20.8 ± 1.79  97.8 ± 3.67 57 2.79 ± 0.76 37.7 ± 6.69 5.88 ± 0.79  90.3 ± 2.60 58 14.3 ± 5.43 21.7 ± 5.49 24.3 ± 5.67  93.7 ± 4.48 59 22.6 ± 6.31 25.7 ± 0.33 26.0 ± 2.61 105.3 ± 4.33 62 17.0 ± 3.31 25.0 ± 0.58 23.6 ± 1.09 108.7 ± 3.67 64 6.96 ± 0.27 48.7 ± 0.33 3.51 ± 0.16 100.7 ± 4.98 66 29.0 ± 4.29 15.5 ± 1.50 167.9 ± 8.15   77.3 ± 0.88 75 >20 μM 2.00 1261 ± 74.6   69.0 ± 6.03 76 >20 μM 2.00 922.9 ± 31.9   81.0 ± 6.66 77 388.9 ± 74.0  85.0 ± 2.52  1148 ± 220.5  65.3 ± 6.44 78 89.3 ± 14.6 94.7 ± 2.40 973.1 ± 135.7  89.7 ± 4.84

The in vitro test results of Table 5 show that representative Compounds of the Invention activate these receptors as partial to full agonists. Compounds of the Invention are therefore expected to be useful to treat Conditions, particularly pain, that are responsive to the activation of one or more opioid receptors.

Example 24

The following TABLE 6 provides results on the activity response of certain Compounds of the Invention at the μ- and κ-opioid receptors determined as described above using HEK-293 cells.

TABLE 6 Activity Response of Certain Compounds of the Invention Opioid Receptor Compd. μ κ No. EC₅₀ (nM) E_(max) (%) EC₅₀ (nM) E_(max) (%) 95 >20 μM  1.00 96 14.4 ± 2.55 14.2 ± 2.50 97 28.1 ± 1.54 90.3 ± 0.88 98  1295 ± 222.9 26.7 ± 0.67 99 243.6 ± 29.5  73.3 ± 1.20 100  1095 ± 187.8 50.3 ± 0.33 101 1.58 ± 0.22 96.7 ± 2.19 102 655.4 ± 128.3 49.5 ± 4.94 103 389.3 ± 73.3  68.0 ± 9.54 104 2932 ± 1083 52.0 ± 3.51 107 10.8 ± 0.83 94.7 ± 0.33 108 12.4 ± 2.76 83.3 ± 5.17 137.6 ± 35.0 40.6 ± 3.30 109 80.4 ± 14.1 70.4 ± 0.98 110  8413 ± 955.8 35.2 ± 0.76 111  3167 ± 492.8 35.1 ± 1.06 112 10172 ± 241.1  38.8 ± 1.54 113 >20 μM −1.00 ± 0.00  114 8437 ± 1544 23.0 ± 1.54 115 917.0 ± 131.3 35.0 ± 1.14 116 3.45 ± 0.16 23.6 ± 1.05 117 >20 μM −1.21 ± 0.21  118 174.5 ± 9.90  13.2 ± 1.17 119 13.4 ± 4.19 18.4 ± 1.55 120 631.3 ± 152.0 22.7 ± 1.92 121 1088 ± 70.2  29.3 ± 1.78 122 >20 μM 0.33 ± 0.67 123  2919 ± 377.8 10.5 ± 1.07 124 >20 μM 1.93 ± 2.93 125 >20 μM −1.00 126 >20 μM −1.00 127 >20 μM −1.00 128 >20 μM −1.00 129 >20 μM  1.00 130 >20 μM  0.00 131 >20 μM  1.00 132 >20 μM −1.00 133 14.2 ± 3.73 29.8 ± 1.22 134 92.0 ± 19.7 9.53 ± 0.90 135 10.3 ± 1.03 19.7 ± 0.89 136 >20 μM −0.33 ± 0.67  137  0.45 ± 0.061 55.5 ± 0.31 138  0.71 ± 0.068 52.4 ± 1.72 139 >20 μM −1.00 140 >20 μM 0.33 ± 0.67 141 1.17 ± 0.10 47.4 ± 0.83 142 >20 μM  0.00 143 2.09 ± 0.45 39.9 ± 1.01 145 >20 μM −1.00 146 2.22 ± 0.41 28.3 ± 1.13 147 >20 μM −1.00 148 >20 μM  1.00 149 >20 μM  0.00 150 >20 μM −1.00 151 >20 μM −1.00 152 >20 μM 4.45 ± 3.18

The in vitro test results of Table 6 show that representative Compounds of the Invention activate these receptors as partial to full agonists. Compounds of the Invention are therefore expected to be useful to treat Conditions, particularly pain, that are responsive to the activation of one or more opioid receptors.

Having now fully described this invention, it will be understood by those of ordinary skill in the art that the same can be performed within a wide and equivalent range of conditions, formulations and other parameters without affecting the scope of the invention or any embodiment thereof.

Other embodiments of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the invention being indicated by the following claims.

All patents, patent applications, and publications cited herein are fully incorporated by reference herein in their entirety. 

What is claimed is:
 1. A compound of Formula I:

or a pharmaceutically acceptable salt or solvate thereof, wherein: R¹ is hydrogen, hydroxy, halo, cyano, carboxy, or aminocarbonyl; or alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; or —O-PG, wherein PG is a hydroxyl protecting group; R² is (a) hydrogen or carboxamido; or (b) alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, alkylcarbonyl, alkoxycarbonyl, (arylalkoxy)carbonyl, or (heteroarylalkoxy)carbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; R³ is hydrogen, hydroxy, or halo; or alkoxy, alkylamino, or dialkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; each R⁴ is independently selected from the group consisting of hydroxy, halo, alkyl, haloalkyl, cyano, nitro, amino, alkylamino, dialkylamino, carboxy, alkoxy, and alkoxycarbonyl; G is selected from the group consisting of G¹, G², G³, and G⁴, wherein G¹ is —C(═O)OR⁵, G² is —C(═O)NR⁶R⁷, G³ is —NR⁸R⁹, and G⁴ is —CN, wherein R⁵ is hydrogen or alkyl; R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylkyl, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, and guanidinoalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; or R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; R⁸ and R⁹ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, (cycloalkyl)-C(═O)—, (cycloalkenyl)-C(═O)—, heterocyclo-C(═O)—, aryl-C(═O)—, heteroaryl-C(═O)—, (cycloalkyl)alkyl-C(═O)—, (cycloalkenyl)alkyl-C(═O)—, (heterocyclo)alkyl-C(═O)—, arylalkyl-C(═O)—, (heteroaryl)alkyl-C(═O)—, (cycloalkyl)-NR¹⁰—C(═O)—, (cycloalkenyl)-NR¹⁰—C(═O)—, heterocyclo-NR¹⁰—C(═O)—, aryl-NR¹⁰—C(═O)—, heteroaryl-NR¹⁰—C(═O)—, (cycloalkyl)alkyl-NR¹⁰—C(═O)—, (cycloalkenyl)alkyl-NR¹⁰—C(═O)—, (heterocyclo)alkyl-NR¹⁰—C(═O)—, arylalkyl-NR¹⁰—C(═O)—, (heteroaryl)alkyl-NR¹⁰—C(═O)—, (cycloalkyl)-SO₂—, (cycloalkenyl)-SO₂—, heterocyclo-SO₂—, aryl-SO₂—, heteroaryl-SO₂—, (cycloalkyl)alkyl-SO₂—, (cycloalkenyl)alkyl-SO₂—, (heterocyclo)alkyl-SO₂—, arylalkyl-SO₂—, (heteroaryl)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, and guanidinoalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; wherein R¹⁰ is hydrogen or alkyl; and R^(10a) is alkyl, alkenyl, or alkynyl; or R¹⁰ and R^(10a) together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring.
 2. A compound of Formula IA:

or a pharmaceutically acceptable salt or solvate thereof, wherein: R¹ is hydrogen, hydroxy, halo, cyano, carboxy, or aminocarbonyl; or alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; or —O-PG, wherein PG is a hydroxyl protecting group; R² is (a) hydrogen or carboxamido; or (b) alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, alkylcarbonyl, alkoxycarbonyl, (arylalkoxy)carbonyl, or (heteroarylalkoxy)carbonyl, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, alkyl, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; R³ is hydrogen, hydroxy, or halo; or alkoxy, alkylamino, or dialkylamino, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups; each R⁴ is independently selected from the group consisting of hydroxy, halo, alkyl, haloalkyl, cyano, nitro, amino, alkylamino, dialkylamino, carboxy, alkoxy, and alkoxycarbonyl; G is selected from the group consisting of G¹, G², G³, and G⁴, wherein G¹ is —C(═O)OR⁵, G² is —C(═O)NR⁶R⁷, G³ is —NR⁸R⁹, and G⁴ is —CN, wherein R⁵ is hydrogen or alkyl; R⁶ and R⁷ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, guanidinoalkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; or R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; R⁸ and R⁹ are each independently selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclo, aryl, heteroaryl, (cycloalkyl)alkyl, (cycloalkenyl)alkyl, (heterocyclo)alkyl, arylalkyl, heteroarylalkyl, (cycloalkyl)-C(═O)—, (cycloalkenyl)-C(═O)—, heterocyclo-C(═O)—, aryl-C(═O)—, heteroaryl-C(═O)—, (cycloalkyl)alkyl-C(═O)—, (cycloalkenyl)alkyl-C(═O)—, (heterocyclo)alkyl-C(═O)—, arylalkyl-C(═O)—, (heteroaryl)alkyl-C(═O)—, (cycloalkyl)-NR¹⁰—C(═O)—, (cycloalkenyl)-NR¹⁰—C(═O)—, heterocyclo-NR¹⁰—C(═O)—, aryl-NR¹⁰—C(═O)—, heteroaryl-NR¹⁰—C(═O)—, (cycloalkyl)alkyl-NR¹⁰—C(═O)—, (cycloalkenyl)alkyl-NR¹⁰—C(═O)—, (heterocyclo)alkyl-NR¹⁰—C(═O)—, arylalkyl-NR¹⁰—C(═O)—, (heteroaryl)alkyl-NR¹⁰—C(═O)—, (cycloalkyl)-SO₂—, (cycloalkenyl)-SO₂—, heterocyclo-SO₂—, aryl-SO₂—, heteroaryl-SO₂—, (cycloalkyl)alkyl-SO₂—, (cycloalkenyl)alkyl-SO₂—, (heterocyclo)alkyl-SO₂—, arylalkyl-SO₂—, (heteroaryl)alkyl-SO₂—, R^(10a)—C(═O)—, R^(10a)—NR¹⁰—C(═O)—, R^(10a)—SO₂—, aminoalkyl, (alkylamino)alkyl, (dialkylamino)alkyl, (aminocarbonyl)alkyl, (alkylaminocarbonyl)alkyl, (dialkylaminocarbonyl)alkyl, carboxyalkyl, (alkoxycarbonyl)alkyl, guanidinoalkyl, hydroxyalkyl, and alkoxyalkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, aryl, and heteroaryl portions are optionally substituted with one or more R⁴ groups; wherein R¹⁰ is hydrogen or alkyl; and R^(10a) is alkyl, alkenyl, or alkynyl; or R¹⁰ and R^(10a) together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring; and R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted heterocyclic ring.
 3. The compound of claim 2, having Formula II:

or a pharmaceutically acceptable salt or solvate thereof, wherein R¹, R², R³, and G are as defined in claim
 2. 4. The compound of claim 2, having Formula IV or Formula V:

or a pharmaceutically acceptable salt or solvate thereof, wherein R¹, R², R³, and G are as defined in claim
 2. 5. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R¹ is hydrogen, hydroxy, halo, cyano, carboxy, or aminocarbonyl; or alkyl, alkenyl, alkynyl, alkoxy, alkenyloxy, or alkynyloxy, any of which is optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, haloalkyl, amino, alkylamino, dialkylamino, carboxy, alkoxy, alkoxycarbonyl, aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl, wherein said aryl, heteroaryl, heterocyclo, cycloalkyl, and cycloalkenyl are optionally substituted with 1, 2, or 3 independently selected R⁴ groups.
 6. The compound of claim 5, or a pharmaceutically acceptable salt or solvate thereof, wherein R¹ is hydroxy or unsubstituted C₁₋₆ alkoxy.
 7. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R² is cyclopropyl(C₁₋₄)alkyl, cyclobutyl(C₁₋₄)alkyl, cyclopentyl(C₁₋₄)alkyl, or cyclohexyl(C₁₋₄)alkyl, optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl.
 8. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R² is C₁₋₄ alkyl, which is unsubstituted or substituted with 1, 2, or 3 substituents each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl.
 9. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R³ is hydroxy, unsubstituted C₁₋₆ alkoxy, or C₁₋₆ alkoxy substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl.
 10. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein G is G¹, which is —C(═O)OR⁵, wherein R⁵ is hydrogen or C₁₋₄ alkyl.
 11. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein G is G², which is —C(═O)NR⁶R⁷, wherein R⁶ is hydrogen or C₁₋₆ alkyl, and R⁷ is selected from the group consisting of hydrogen, C₁₋₆ alkyl, C₂₋₆ alkenyl, C₂₋₆ alkynyl, C₃₋₇ cycloalkyl, C₃₋₇ cycloalkenyl, 5- or 6-membered heterocyclo, phenyl, 5- or 6-membered heteroaryl, (C₃₋₇ cycloalkyl)(C₁₋₆)alkyl, (C₃₋₇ cycloalkenyl)(C₁₋₆)-alkyl, (5- or 6-membered heterocyclo)(C₁₋₆)-alkyl, phenyl(C₁₋₆)alkyl, (5- or 6-membered heteroaryl)(C₁₋₆)alkyl, amino(C₁₋₆)alkyl, (C₁₋₆ alkylamino)(C₁₋₆)alkyl, (di(C₁₋₆ alkyl)amino)(C₁₋₆)alkyl, (aminocarbonyl)(C₁₋₆)alkyl, (C₁₋₆ alkylaminocarbonyl)(C₁₋₆)alkyl, (di(C₁₋₆)alkylaminocarbonyl)(C₁₋₆)alkyl, carboxy(C₁₋₆)alkyl, (C₁₋₆ alkoxycarbonyl)(C₁₋₆)alkyl, guanidino(C₁₋₆)alkyl, hydroxy(C₁₋₆)alkyl, and (C₁₋₆)alkoxy(C₁₋₆)alkyl, wherein the cycloalkyl, cycloalkenyl, heterocyclo, phenyl, and heteroaryl portions are optionally substituted with one or more R⁴ groups.
 12. The compound of claim 11, or a pharmaceutically acceptable salt or solvate thereof, wherein G² is selected from the group consisting of

wherein m is independently 0, 1, 2, or 3, and R¹¹, R¹², and R¹³ are each independently selected from the group consisting of hydrogen, hydroxy, halogen, and C₁₋₄ alkyl.
 13. The compound of claim 11, or a pharmaceutically acceptable salt or solvate thereof, wherein G² is selected from the group consisting of


14. The compound of compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R⁶ and R⁷ together with the nitrogen atom to which they are attached form an optionally substituted 3-7 membered heterocyclic ring.
 15. The compound of claim 14, or a pharmaceutically acceptable salt or solvate thereof, wherein G is G², wherein R⁶ and R⁷ together with the nitrogen atom to which they are attached form a pyrrolidinyl, a piperidinyl, a piperazinyl, a morpholinyl, a thiomorpholinyl, or a dioxidothiomorpholinyl ring, which is unsubstituted or substituted with 1, 2, or 3 substituents each independently selected from the group consisting of hydroxy, halo, C₁₋₄ alkyl, halo(C₁₋₄)alkyl, cyano, nitro, amino, aminocarbonyl, (C₁₋₄ alkylamino)carbonyl, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, C₁₋₄ alkoxycarbonyl, C₃₋₆ cycloalkyl, C₃₋₆ cycloalkenyl, pyrrolidin-1-yl, piperidin-1-yl, and phenyl, wherein said phenyl is optionally substituted with 1, 2, or 3 independently selected R⁴ groups.
 16. The compound of claim 15, or a pharmaceutically acceptable salt or solvate thereof, wherein G² is selected from the group consisting of


17. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein G is G³, which is —NR⁸R⁹, wherein R⁸ and R⁹ both are hydrogen; or R⁸ is hydrogen, and R⁹ is

or R⁸ and R⁹ are both

wherein each R¹⁵ is independently selected from the group consisting of hydrogen, halogen, and C₁₋₄alkyl.
 18. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein G is G³, wherein R⁸ and R⁹ together with the nitrogen atom to which they are attached form an optionally substituted 3-7 membered heterocyclic ring.
 19. The compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein R¹ is hydroxy or unsubstituted C₁₋₆ alkoxy; R² is unsubstituted C₁₋₄ alkyl; C₁₋₄ alkyl substituted with halo(C₁₋₄)alkyl; or cyclopropyl(C₁₋₄)alkyl, cyclobutyl(C₁₋₄)alkyl, cyclopentyl(C₁₋₄)alkyl, or cyclohexyl(C₁₋₄) alkyl, optionally substituted with 1, 2, or 3 substituents, each independently selected from the group consisting of hydroxy, C₁₋₄ alkyl, halo, halo(C₁₋₄)alkyl, amino, C₁₋₄ alkylamino, di(C₁₋₄)alkylamino, carboxy, C₁₋₄ alkoxy, and C₁₋₄ alkoxycarbonyl; and R³ is hydrogen or hydroxy.
 20. The compound of claim 2, selected from the group consisting of (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2-guanidinoethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(4-guanidinobutyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-methyl 12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylate; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylic acid; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxylic acid; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)-cyclopenta[a]naphthalene-2-carboxylic acid; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-yl)(pyrrolidin-1-yl)methanone; ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-yl)(pyrrolidin-1-yl)methanone; (2R,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a-ol; (2R,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-3a,8-diol; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carbonitrile; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carbonitrile; (2S,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-8-methoxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a-ol; (2S,3aS,4R,9bS)-2-amino-12-(cyclopropylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-3a,8-diol; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-benzyl-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-benzyl-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-isobutyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(4-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(4-chlorobenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(4-fluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,5-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(piperidin-1-yl)methanone; ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(piperidin-1-yl)methanone; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(morpholino)methanone; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N,N-diethyl-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N,N-dimethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2-amino-2-oxoethyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; 2-((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamido)acetic acid; (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-(2,2,2-trifluoroethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-(2,2,2-trifluoroethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; N-((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)benzamide; (2R,3aS,4R,9bS)-2-(benzylamino)-12-(cyclopropylmethyl)-2,3,4,5-tetrahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a,8(1 H)-diol; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-2-(dibenzylamino)-2,3,4,5-tetrahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-3a,8(1 H)-diol; N-((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)benzamide; 1-((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)-3-phenylurea; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(3-morpholinopropyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(4-phenylpiperazin-1-yl)methanone; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(thiomorpholino)methanone; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(4-(pyrrolidin-1-yl)piperidin-1-yl)methanone; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-3-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-4-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(3-morpholinopropyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(pyridin-3-ylmethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(1,1-dioxidothiomorpholino)methanone; N-((2R,3aS,4R,9bR)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)benzenesulfonamide; ((2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(4-phenylpiperazin-1-yl)methanone; (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2,4-dihydroxybenzyl)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-(3,4-dichlorobenzyl)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-3a,8-dihydroxy-N-isobutyl-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-benzyl-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-benzyl-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-(2,4-dichlorobenzyl)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-N-phenethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; N-((2S,3aS,4R,9bR)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)benzamide; N-((2R,3aS,4R,9bR)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)benzamide; ((2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(morpholino)methanone; (2R,3aS,4R,9bS)-3a,8-dihydroxy-12-methyl-N-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2-(dimethylamino)ethyl)-3a,8-dihydroxy-N,12-dimethyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-3a,8-dihydroxy-N-(2-hydroxyethyl)-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-3a,8-dihydroxy-N-(2-hydroxyethyl)-12-methyl-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-N2-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2,8-dicarboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-N2-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2,8-dicarboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-N2-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2,8-dicarboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a-hydroxy-N2-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2,8-dicarboxamide; (2R,3aS,4R,9bS)-8-cyano-12-(cyclopropylmethyl)-3a-hydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-8-cyano-12-(cyclopropylmethyl)-3a-hydroxy-N-(4-methylbenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-8-cyano-12-(cyclopropylmethyl)-3a-hydroxy-N-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-8-cyano-12-(cyclopropylmethyl)-3a-hydroxy-N-(2-morpholinoethyl)-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,3-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(3-chloro-2-methylbenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(3-chloro-4-methylbenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2-chloro-4-hydroxybenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(3-chloro-4-hydroxybenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(4-chlorophenethyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-dichlorophenethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dichlorophenethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; ((2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalen-2-yl)(4-isopropylpiperazin-1-yl)methanone; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(2-(piperidin-1-yl)ethyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2-((2S,6R)-2,6-dimethylmorpholino)ethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2-((2S,6R)-2,6-dimethylmorpholino)ethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(4-chloro-2-fluorobenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-(3-chloro-4-methylbenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-N-(3-chloro-2-methylbenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b -(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,5-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,3-dihydroxybenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(3-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,5-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,4-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2S,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,4-difluorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-N-(2-chlorobenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-3a,8-dihydroxy-N-(2-hydroxybenzyl)-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(3,5-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; (2R,3aS,4R,9bS)-12-(cyclopropylmethyl)-N-(2,5-dichlorobenzyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; and (2R,3aS,4R,9bS)-N-(3-chlorobenzyl)-12-(cyclopropylmethyl)-3a,8-dihydroxy-1,2,3,3a,4,5-hexahydro-4,9b-(epiminoethano)cyclopenta[a]naphthalene-2-carboxamide; or a pharmaceutically acceptable salt or solvate thereof.
 21. A pharmaceutical composition, comprising a therapeutically effective amount of a compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, and one or more pharmaceutically acceptable carriers.
 22. A method of treating pain, in a patient, comprising administering an effective amount of a compound of claim 2, or a pharmaceutically acceptable salt or solvate thereof, to the patient in need thereof.
 23. The method of claim 22, wherein said pain is acute pain, chronic pain or surgical pain, and said chronic pain is selected from the group consisting of neuropathic pain, postoperative pain, and inflammatory pain.
 24. A method of modulating one or more opioid receptors in a patient, comprising administering to the patient an effective amount of a compound as claimed in claim 2, or a pharmaceutically acceptable salt or solvate thereof, wherein a μ- or κ-opioid receptor is modulated, or both the μ- and κ-opioid receptors are modulated. 